High nitrogen input is important to guarantee higher production and essential oil yield. Ecologically, it is necessary to determine whether reduced nitrogen levels have an impact on the medicinal herbs’ productions. In the recent research, the influences of reduced nitrogen supply on the agronomic traits and essential oil productivity of chamomile have been evaluated. The experiment pots were set, and five levels of nitrogen fertilizer Urea (0, 25, 50, 75, and 100 kg N/ha) have been applied to the soil during the growing season. As a result, Chamomile growth, flower, essential oil content and yield were significantly increased when urea was applied as 75 kg N/ha. As a result, this research illustrates that reducing nitrogen supply might be a promising environmentally friendly way and an alternate strategy for improving the essential oil of chamomile and perhaps other medicinal plants, while also helping the environment.