Influence of Nonthermal Atmospheric Plasma-Activated Water on the Structural, Optical, and Biological Properties of Aspergillus brasiliensis Spores

Ki, Se Hoon; Noh, Hyeongjin; Ahn, Geum Ran; Kim, Seong Hwan; Kaushik, Nagendra Kumar; Choi, Eun Ha; Lee, Geon Joon

doi:10.3390/app10186378

Cited by 17 publications

(9 citation statements)

References 62 publications

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“…To evaluate the extent of species involvement in the microbial inactivation of PAW, Su et al (2018) demonstrated that • OH, • NO, and H 2 O 2 were the main species that changed morphology and destroyed and degraded the RNA and protein structure, causing virus inactivation. Moreover, Guo et al (2018) suggested that 1 O 2 was the main functional agent in PAW for bacteriophage inactivation, and Ki et al (2020) reported that the long‐lived reactive species in PAW had a good inactivation effect on melanized fungal spores. In our study, the results demonstrated that long‐lived species such as

{NO}_{2}^{-}

{NO}_{3}^{-}

were key players in bacterial inactivation, and the presented data gave evidence that

O_{2}^{\cdot -}

was a significant contributor to antimicrobial activity of PAW.…”

Section: Resultsmentioning

confidence: 99%

The optimization of plasma activated water ( PAW ) generation and the inactivation mechanism of PAW on Escherichia coli

Han

Chen

et al. 2022

Food Processing Preservation

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Plasma activated water (PAW) generated by nonthermal plasma, an acidified solution containing a variety of reactive species, has become a new decontamination approach because of its bactericidal ability. This study investigated the effects of various generation conditions of PAW on bactericidal activities against Escherichia coli and its physicochemical properties. PAW was prepared by ultrapure water with dielectric barrier discharge (DBD) plasma. The optimal generation condition of PAW was 30 min plasma activation time, 20 ml solution volume, 15.2 kV input voltage, and 5.5 kHz frequency operation. Under various mimicked liquid analytics, nitrate, nitrite, superoxide anion radicals, and strong acidification play significant roles in the antimicrobial properties of PAW. Therefore, these findings provide a basis for the PAW inactivation mechanism, and PAW can be a promising alternative to traditional sanitizers for food safety and preservation. Practical applications Plasma‐activated water (PAW) is an emerging non‐thermal technology that could be applied in the food industry. The liquid creates an acidic environment which results in changes of the redox potential, conductivity, and the formation of reactive oxygen and nitrogen species. As a result, PAW has different chemical composition than water and can serve as an alternative method for microbial disinfection. This study presents physicochemical properties and bactericidal effects of PAW under different generation conditions. Moreover, the inactivation mechanism of Escherichia coli by PAW was analyzed using various mimicked solutions. This investigation will help to have a better understanding of PAW technology and extend the potential applications in the agri‐food industry in the future.

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{NO}_{2}^{-}

{NO}_{3}^{-}

were key players in bacterial inactivation, and the presented data gave evidence that

O_{2}^{\cdot -}

was a significant contributor to antimicrobial activity of PAW.…”

Section: Resultsmentioning

confidence: 99%

The optimization of plasma activated water ( PAW ) generation and the inactivation mechanism of PAW on Escherichia coli

Han

Chen

et al. 2022

Food Processing Preservation

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“…As shown in Figure 5 d, the absorption spectra of the PAW and PA-Mg-W exhibited an absorption peak at 201 nm. As the reference nitrite and nitrate solutions have absorption peaks at 209 and 201 nm, respectively [ 12 , 46 ], the observed 201-nm absorption band indicates that nitrate ions greatly outnumber nitrite ions. Next, the nitrate absorption peak intensity at 300 nm was higher in the PA-Mg-W than in the PAW, as shown in Figure 5 a,b.…”

Section: Resultsmentioning

confidence: 99%

“…That is, Ascomycota fungi are characterized by gel-like α-1,3 glucans and galactomannoproteins, whereas Basidiomycota fungi are characterized by α-1,3 glucans along with xylomannoproteins [ 54 ]. In a previous work, we demonstrated that PAW influences the spore viability and cell wall integrity of the Ascomycota fungus, Aspergillus brasiliensis , which has melanized fungal spores [ 12 ]. We wondered whether PAW would be applicable to Basidiomycota fungi.…”

Section: Resultsmentioning

confidence: 99%

“…Therefore, PAW can also destroy the cells of Basidiomycota fungi encapsulated by a relatively thick cell wall. These results demonstrated that the PAW produced by the atmospheric-pressure Ar plasma jet is detrimental not only to Ascomycota fungal spores which were produced by conidiophores [ 12 ] but also to Basidiomycota yeast cells which were produced by budding. From the viewpoint of applications such as sanitary means, it is meaningful that the two taxonomically different major groups of fungi are susceptible enough to the PAW produced by the plasma jet.…”

Section: Resultsmentioning

confidence: 99%

“…Since the emergence of plasma medicine and plasma chemistry, PAW has attracted considerable interest for various applications in biological and medical science as well as in the water, agriculture, and food industries [ 1 , 2 ]. The mechanism of fungal spore inactivation by PAW is of particular interest because PAW can kill a larger volume of fungi than a direct plasma jet can [ 11 , 12 ]. To make PAW a practical tool, we need not only a fundamental understanding of its antifungal mechanism but also methods for handling the RONS in PAW in field applications.…”

Section: Introductionmentioning

confidence: 99%

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Nitrate Capture Investigation in Plasma-Activated Water and Its Antifungal Effect on Cryptococcus pseudolongus Cells

Lee

Lamichhane

Ahn

et al. 2021

IJMS

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This research investigated the capture of nitrate by magnesium ions in plasma-activated water (PAW) and its antifungal effect on the cell viability of the newly emerged mushroom pathogen Cryptococcus pseudolongus. Optical emission spectra of the plasma jet exhibited several emission bands attributable to plasma-generated reactive oxygen and nitrogen species. The plasma was injected directly into deionized water (DW) with and without an immersed magnesium block. Plasma treatment of DW produced acidic PAW. However, plasma-activated magnesium water (PA-Mg-W) tended to be neutralized due to the reduction in plasma-generated hydrogen ions by electrons released from the zero-valent magnesium. Optical absorption and Raman spectra confirmed that nitrate ions were the dominant reactive species in the PAW and PA-Mg-W. Nitrate had a concentration-dependent antifungal effect on the tested fungal cells. We observed that the free nitrate content could be controlled to be lower in the PA-Mg-W than in the PAW due to the formation of nitrate salts by the magnesium ions. Although both the PAW and PA-Mg-W had antifungal effects on C. pseudolongus, their effectiveness differed, with cell viability higher in the PA-Mg-W than in the PAW. This study demonstrates that the antifungal effect of PAW could be manipulated using nitrate capture. The wide use of plasma therapy for problematic fungus control is challenging because fungi have rigid cell wall structures in different fungal groups.

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Essentials and Pertinence of Cold Plasma in Essential Oils, Metal–Organic Frameworks and Agriculture

Khan,

Akram,

Naeem

et al. 2024

Food Science & Nutrition

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Cold atmospheric pressure plasma (CAPP) comprises an ensemble of ionized gas, neutral particles, and/or reactive species. Electricity is frequently used to produce CAPP via a variety of techniques, including plasma jets, corona discharges, dielectric barrier discharges, and glow discharges. The type and flow rates of the carrier gas(es), temperature, pressure, and vacuum can all be altered to control the desired properties of the CAPP. Since a few decades ago, CAPP has become a widely used technology with applications in every walk of life. The plasma activated liquid mediums like water, ethanol, and methanol have been merged as novel sterilizers. With recent advancements in material science, particularly work on metal–organic frameworks (MOFs), essential oils, and agricultural technologies, CAPP has become a vital component of these advancements. Likewise, CAPP has been found as a green and benign technology to induce early seed germination and plant development. This review covers the critical components of CAPP, the production of reactive oxygen and nitrogen species, and mechanisms by which CAPP‐based technologies are applied to agricultural products, MOFs, and essential oils.

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Influence of Nonthermal Atmospheric Plasma-Activated Water on the Structural, Optical, and Biological Properties of Aspergillus brasiliensis Spores

Cited by 17 publications

References 62 publications

The optimization of plasma activated water ( PAW ) generation and the inactivation mechanism of PAW on Escherichia coli

The optimization of plasma activated water ( PAW ) generation and the inactivation mechanism of PAW on Escherichia coli

Nitrate Capture Investigation in Plasma-Activated Water and Its Antifungal Effect on Cryptococcus pseudolongus Cells

Essentials and Pertinence of Cold Plasma in Essential Oils, Metal–Organic Frameworks and Agriculture

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