Influence of oestradiol on induction of EROD activity in roach (Rutilus rutilus L.)

O’Hare, Dermot; Siddall, Roy; Robotham, P. W. J.; Gill, R. A.

doi:10.1016/0045-6535(95)00035-7

Cited by 13 publications

(10 citation statements)

References 13 publications

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“…A significant difference between single and binary exposures was found following the qPCR assay in which the binary treatment resulted in a significantly higher expression of cyp1A. This is in contrast to studies that have shown that in roach Rutilus rutilus (L.), E2 exposure attenuated EROD induction following exposure to a PAH, suggesting an inhibitory effect (O’Hare et al , 1995). The relative concentrations are likely to be major determinants of the response.…”

Section: Discussioncontrasting

confidence: 74%

A cDNA microarray for the three‐spined stickleback, Gasterosteus aculeatus L., and analysis of the interactive effects of oestradiol and dibenzanthracene exposures

Geoghegan

Katsiadaki

Williams

et al. 2008

Journal of Fish Biology

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The stickleback is a useful model species for aquatic toxicology and endocrinology and with the recent sequencing of its genome, ecotoxicogenomics. The effects of treatment on male and female sticklebacks with the polycyclic aromatic hydrocarbon (PAH), dibenzanthracene (DbA), the female hormone, 17b-oestradiol (E2) and a binary mixture of the two were investigated. Quantitative RT-PCR assays were carried out for cytochrome P450 1A (cyp1A), vitellogenin (VTG) and oestrogen receptor alpha, and cyp1A enzyme activity was assessed by measurement of ethoxyresorufin-o-deethylase (EROD) activity , VTG and spiggin protein by enzyme-linked immunosorbent assay. A stickleback cDNA microarray consisting of 9692 clones was developed and used to assess gene expression responses to the treatments. Induction of cyp1A mRNA and EROD activity was seen in both sexes in response to DbA and a further induction of cyp1A found with the binary treatment. VTG mRNA was induced in male fish exposed to E2 and the binary mixture, while for females, statistically significant induction was seen only with the binary mixture. Similar behaviour was found with zona pellucida and chorion protein mRNA using the microarray. ERa mRNA was induced with E2 and the binary mixture in both sexes. The microarray showed additional transcripts that were differentially regulated by these treatments, which provide novel candidates for biomarker development and mechanistic studies.

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Section: Discussioncontrasting

confidence: 74%

A cDNA microarray for the three‐spined stickleback, Gasterosteus aculeatus L., and analysis of the interactive effects of oestradiol and dibenzanthracene exposures

Geoghegan

Katsiadaki

Williams

et al. 2008

Journal of Fish Biology

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“…The role of estrogens in regulating the hepatic cytochrome P450 system has been studied and a possible inhibitory effect of estrogens has been suggested. Administration of estradiol to juvenile Salvelinus fontinalis and Oncorhynchus mykiss resulted in significant decreases in monooxygenase activities (Stegeman et al, 1982); coinjection of both estradiol-17 and -naphthoflavone to cyprinid, Rutilus rutilus, resulted in almost 50% decreases in both basal and induced EROD levels (O'Hare et al, 1995). However, the possible spawning migration of the fish from or to the studied area must be kept in mind and may also explain the greater variability that was observed in spring.…”

Section: Discussionmentioning

confidence: 93%

Statistical Analysis of Cyprinid Ethoxyresorufin-O-deethylase Data in a Large French Watershed

Flammarion

Migeon

Garric

1998

Ecotoxicology and Environmental Safety

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“…Using the above selection criteria, 94 papers were retained for the analysis of EROD control/reference data [12–105], and 27 of these papers were selected for further analysis of EROD dose‐response relationships [12–38]. The papers chosen for analysis of the EROD control/reference data set had a total of 78 unique author groups (which we equated to individual laboratories), 42 different genera of fish, and 21 different families of fish.…”

Section: Resultsmentioning

confidence: 99%

Statistical analysis of cytochrome P4501A biomarker measurements in fish

Oris

Roberts

2007

Enviro Toxic and Chemistry

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Abstract-Induction of the cytochrome P4501A (CYP1A) enzyme system in fish is a common biomarker of exposure to aromatic hydrocarbons. Induction of CYP1A can be measured at a number of steps in the transcription-translation-functional protein pathway using a variety of techniques. The present study examined the range of these measurements from 94 published papers in an attempt to examine the statistical characteristics of each method. Cytochrome P4501A induction, as measured by catalytic ethoxyresorufin-O-deethylase (EROD) activity, protein levels (enzyme-linked immunosorbent assay, Western blot analysis, and immunohistochemistry), and mRNA levels (Northern blot analysis and reverse transcription-polymerase chain reaction), was analyzed. When possible, the variance structure, effect size determination, and dose-response modeling of each method of measurement in the laboratory and field were examined. Conclusions from this analysis include: 1) Because of interlaboratory and interspecies variability, general end-point determinations will need to be defined in terms of the statistically detectable fold-change of measurements relative to control or reference values, and 2) fold-change in EROD activity provides the most robust measure of the dose responsiveness of aromatic hydrocarbons within specific chemical classes (e.g., polycyclic aromatic hydrocarbons). The relationship between the ability to measure statistical differences in induction level and the biological significance of those measurements has yet to be defined. To utilize these biomarkers in a risk assessment context, this relationship must be addressed at the scientific and management levels.

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Influence of oestradiol on induction of EROD activity in roach (Rutilus rutilus L.)

Cited by 13 publications

References 13 publications

A cDNA microarray for the three‐spined stickleback, Gasterosteus aculeatus L., and analysis of the interactive effects of oestradiol and dibenzanthracene exposures

A cDNA microarray for the three‐spined stickleback, Gasterosteus aculeatus L., and analysis of the interactive effects of oestradiol and dibenzanthracene exposures

Statistical Analysis of Cyprinid Ethoxyresorufin-O-deethylase Data in a Large French Watershed

Statistical analysis of cytochrome P4501A biomarker measurements in fish

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