Influence of operational conditions on the performance of biogas bioconversion into ectoines in pilot bubble column bioreactors

Rodero, María del Rosario; Herrero-Lobo, Raquel; Pérez, Víctor Alfonsín; Muñoz, Raúl

doi:10.1016/j.biortech.2022.127398

Cited by 12 publications

(2 citation statements)

References 42 publications

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“…R-PETCs have been used as electronic biosensors for detecting soil herbicide concentrations 201 , as well as powering a microprocessor commonly used for Internet-of-Things devices 40 , demonstrating that R-PETCs can be a sustainable replacement for batteries. In industry, pilot-scale studies are underway utilising photosynthetic organisms for large-scale production of vaccines 202 , therapeutics 203 , biomass 204 and chemicals 205 . Whilst not utilising R-PETCs, these demonstrate the commercial efficacy of photosynthetic biotechnologies.…”

Section: Discussionmentioning

confidence: 99%

Rewiring photosynthetic electron transport chains for solar energy conversion

Lawrence,

Egan,

Hoefer

et al. 2023

Nat Rev Bioeng

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Photosynthetic organisms have evolved versatile electron transport chains capable of the efficient conversion of solar energy into chemical energy. Researchers can engineer these electron transport pathways to drive new-to-nature processes in a class of systems we term "rewired photosynthetic electron transport chains" (R-PETCs). R-PETCs can be used for the light-driven production of electricity or chemicals and exhibit numerous advantages over the synthetic systems widely used for these applications, including enhanced stability, versatility, and sustainability. In this review we summarise the current state of R-PETC research, highlighting major advances alongside outstanding research problems. We also provide descriptions of R-PETC development, outlining the different classes of R-PETCs, the research tools used in their construction, and the key design considerations important for achieving high-performances. Finally, we identify future avenues for R-PETC research which we expect will enhance performances sufficiently to make these systems suitable for a variety of real-world applications.

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Section: Discussionmentioning

confidence: 99%

Rewiring photosynthetic electron transport chains for solar energy conversion

Lawrence,

Egan,

Hoefer

et al. 2023

Nat Rev Bioeng

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“…The extensive work performed by Cantera and coworkers demonstrated the feasibility of pure and mixed methanotrophic bacterial cultures for bioconverting CH4 into ectoines such as ectoine and hydroxyectoine(Cantera et al, 2020). This work was followed by Rodero and coworkers, which demonstrated the robustness of this biogas bioconversion process under long term and continuous operation in bubble column bioreactors equipped with internal gas recirculation(Rodero et al, 2022). In addition, the most recent investigations revealed that the biomilking process in methanotrophic bacteria was extremely effective with > 70 % of the intracellular ectoine released under very low empty bed residence times (EBRT) (<10 min)(Rodero and Muñoz, 2021).Hence, exploring the scalability of biogas-to-fine chemicals schemes like the one herein presented would definitely impact positively the implementation of biogas bioconversion processes, showing that processes based on methanotrophic bacteria are not only restricted to the production of low added-value products.…”

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confidence: 99%

A techno-economic assessment methodology for the production of polyhydroxyalkanoates and ectoine from biogas in waste treatment plants

Martínez¹

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Production of hydroxyectoine from biogas by an engineered strain of Methylomicrobium alcaliphilum using a novel Taylor‐flow bioreactor

Herrero‐Lobo,

Fernández‐González,

Marcos

et al. 2024

J of Chemical Tech & Biotech

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BACKGROUNDThe production of compatible solutes, such as ectoine and hydroxyectoine, is of great interest due to their industrial and biotechnological applications. Methylomicrobium alcaliphilum was genetically engineered to replace a native gene with a heterologous one, aiming to enhance ectoine production. This study focuses on the optimization of bioreactor conditions to maximize the microbial production of these metabolites from methane.RESULTSThe engineered strain (M. alcaliphilum PstEctD) was cultured in a Taylor flow bioreactor under varying gas recirculation flow rates. Increased flow rates enhanced methane consumption, biomass concentration, and ectoine production. The highest production of ectoine (32 mg/g‐VSS) and hydroxyectoine (272 mg/g‐VSS) was observed at a flow rate of 0.7 L min−1, while methane removal efficiency improved from 30% to over 60% as flow rates increased.CONCLUSIONSOptimizing bioreactor conditions, particularly gas recirculation flow rates, significantly improved both the efficiency of methane consumption and the production of ectoine derivatives. This work provides a scalable approach for the sustainable production of compatible solutes from methane, offering potential applications in biotechnological processes utilizing renewable carbon sources. © 2024 The Author(s). Journal of Chemical Technology and Biotechnology published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry (SCI).

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Influence of operational conditions on the performance of biogas bioconversion into ectoines in pilot bubble column bioreactors

Cited by 12 publications

References 42 publications

Rewiring photosynthetic electron transport chains for solar energy conversion

Rewiring photosynthetic electron transport chains for solar energy conversion

A techno-economic assessment methodology for the production of polyhydroxyalkanoates and ectoine from biogas in waste treatment plants

Production of hydroxyectoine from biogas by an engineered strain of Methylomicrobium alcaliphilum using a novel Taylor‐flow bioreactor

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