Influence of oxygen level on oxidative stress response of Bacillus sp. F26 to menadione

Oxidative Stress Induction Is a Rational Strategy to Enhance the Productivity of Antrodia cinnamomea Fermentations for the Antioxidant Secondary Metabolite Antrodin C

“…Various reagents that induce oxidative stress during submerged fermentation affect the growth rate and biomass accumulation of microorganisms and modulate their production of SMs. ,,,, Considering the antioxidant and free radical scavenging activities of antrodin C, we hypothesized that supplementation of A. cinnamomea fermentations with oxidative stress inducers may provoke this fungus to upregulate the biosynthesis of antrodin C. To evaluate this hypothesis, we applied diethyl phthalate (a toxic compound that elevates intracellular ROS levels), menadione (a producer of semiquinone and superoxide radicals), and H 2 O 2 (a microbicidal oxidizing agent) − during A.…”

“…Thus, SM production may be elicited by environmental stimuli, such as biotic and abiotic stressors. One of the most common physiological stress conditions in the habitat of filamentous fungi is oxidative stress that leads to the formation of harmful reactive oxygen species (ROS) in the cells. , ROS, including hydrogen peroxide (H 2 O 2 ), hydroxyl radical (HO · ), and superoxide anions (O 2– ), cause dose-dependent damage to most biomolecules such as DNA, proteins, and lipids. , Microbial cells respond to ROS by initiating scavenging mechanisms, including enzymatic or nonenzymatic systems. , Growing evidence also suggests that ROS play vital physiological roles in the regulation of morphogenesis, differentiation, and secondary metabolism in filamentous fungi. , Thus, modulation of the oxidative stress response of Aspergillus flavus by cinnamaldehyde effectively inhibits radial growth, spore production, and mycelium formation . Methyl jasmonate induced the biosynthesis of ganoderic acids by increasing the intracellular ROS concentration of Ganoderma lucidum .…”

“…One of the most common physiological stress conditions in the habitat of filamentous fungi is oxidative stress that leads to the formation of harmful reactive oxygen species (ROS) in the cells. 3,4 ROS, including hydrogen peroxide (H 2 O 2 ), hydroxyl radical (HO • ), and superoxide anions (O 2− ), cause dose-dependent damage to most biomolecules such as DNA, proteins, and lipids. 3,5 Microbial cells respond to ROS by initiating scavenging mechanisms, including enzymatic or nonenzymatic systems.…”

“…3,4 ROS, including hydrogen peroxide (H 2 O 2 ), hydroxyl radical (HO • ), and superoxide anions (O 2− ), cause dose-dependent damage to most biomolecules such as DNA, proteins, and lipids. 3,5 Microbial cells respond to ROS by initiating scavenging mechanisms, including enzymatic or nonenzymatic systems. 6,7 Growing evidence also suggests that ROS play vital physiological roles in the regulation of morphogenesis, differentiation, and secondary metabolism in filamentous fungi.…”

Breakthrough Model of Recombinant Human-Like Collagen in Immobilized Metal Affinity Chromatography

“…Therefore, histidine-tagged affinity chromatography is most frequently used for the purification of recombinant proteins. Immobilized metal affinity chromatography (IMAC) is mostly used as an effective method for the capture of histidine-tagged proteins from complex protein mixtures [9][10].…”