Influence of Partial Denitrification and Mixotrophic Growth of NOB on Microbial Distribution in Aerobic Granular Sludge

Winkler, Mari-K.H.; Le, Quan H.; Volcke, Eveline

doi:10.1021/acs.est.5b01952

Cited by 62 publications

(16 citation statements)

References 55 publications

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“…The predominance of Nitrobacter sp. in the NOB population in aerobic granules may result from the fact that they can both oxidize nitrite produced during partial denitrification (nitrite-loop) and grow mixotrophically in the presence of acetate (ping-pong), reducing the content of carbon in wastewater [39].…”

Section: Molecular Studiesmentioning

confidence: 99%

Treatment of Liquid Phase of Digestate from Agricultural Biogas Plant in a System with Aerobic Granules and Ultrafiltration

Świątczak

Cydzik‐Kwiatkowska

Zielińska

2019

Water

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Management of digestate from manure co-digestion with a very high chemical oxygen demand (COD) to nitrogen ratio and high nitrogen loads are a major bottleneck in the development of agricultural biogas plants. The liquid phase of digestate mixed with municipal wastewater was treated in aerobic granular sludge batch reactors at cycle lengths (t) of 6 h (GSBR6h), 8 h (GSBR8h), and 12 h (GSBR12h), corresponding to nitrogen loads of 1.6, 1.2, and 0.8 g/(L·d). Thauera sp., Lacibacter sp., Thermanaerothrix sp., and Planctomyces sp. predominated in granules favoring effective granule formation and nitrogen removal. Increasing cycle lengths (t) significantly decreased proteins in soluble fraction of extracellular polymeric substances (EPS) in granules and increased polysaccharides in tightly bound EPS that resulted in higher granule diameters and higher COD removal. In GSBR6h, heterotrophic nitrification/denitrification was very efficient, but ammonium was fully oxidized in the last hour of the cycle. So in further studies, the effluent from GSBR8h was subjected to ultrafiltration (UF) at transmembrane pressures (TMPs) of 0.3, 0.4, and 0.5 MPa. A GSBR8h-UF system (TMP of 0.4 MPa) ensured full removal of total Kjeldahl nitrogen (TKN), suspended solids, and substantial reduction of COD and color with good permeate flux. The NOx-rich (about 250 mg/L), clear permeate can be reused in line with assumptions of modern circular economy.

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Section: Molecular Studiesmentioning

confidence: 99%

Treatment of Liquid Phase of Digestate from Agricultural Biogas Plant in a System with Aerobic Granules and Ultrafiltration

Świątczak

Cydzik‐Kwiatkowska

Zielińska

2019

Water

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“…Denitrification is achieved under anaerobic or anoxic conditions1314. However, sporadic reports on aerobic denitrifiers have induced aerobic denitrification using nitrate-oxidizing agents under aerobic conditions9.…”

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confidence: 99%

Responses of bacterial community structure and denitrifying bacteria in biofilm to submerged macrophytes and nitrate

Zhang

Pang

Wang

et al. 2016

Sci Rep

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Submerged macrophytes play important roles in constructed wetlands and natural water bodies, as these organisms remove nutrients and provide large surfaces for biofilms, which are beneficial for nitrogen removal, particularly from submerged macrophyte-dominated water columns. However, information on the responses of biofilms to submerged macrophytes and nitrogen molecules is limited. In the present study, bacterial community structure and denitrifiers were investigated in biofilms on the leaves of four submerged macrophytes and artificial plants exposed to two nitrate concentrations. The biofilm cells were evenly distributed on artificial plants but appeared in microcolonies on the surfaces of submerged macrophytes. Proteobacteria was the most abundant phylum in all samples, accounting for 27.3–64.8% of the high-quality bacterial reads, followed by Chloroflexi (3.7–25.4%), Firmicutes (3.0–20.1%), Acidobacteria (2.7–15.7%), Actinobacteria (2.2–8.7%), Bacteroidetes (0.5–9.7%), and Verrucomicrobia (2.4–5.2%). Cluster analysis showed that bacterial community structure can be significantly different on macrophytes versus from those on artificial plants. Redundancy analysis showed that electrical conductivity and nitrate concentration were positively correlated with Shannon index and operational taxonomic unit (OTU) richness (log10 transformed) but somewhat negatively correlated with microbial density. The relative abundances of five denitrifying genes were positively correlated with nitrate concentration and electrical conductivity but negatively correlated with dissolved oxygen.

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“…The results showed that Proteobacteria (average relative abundance (RA), 63.4%) was the most abundant phylum across all the samples, followed by Bacteroidetes (average RA, 22.4%), which was also found with a high abundance in other marine MBBRs [45][46][47]. The integration of MA with MBBR significantly [48], and many Bacteroidetes species have been reported to have the capacity for organic carbon degradation [49]. The relatively lower abundance of Nitrospirae in MBBR-MA biofilter samples could be explained by the relatively lower nitrite concentration detected in the MBBR-MA system (Figure 2(b)), because macroalgae also contribute to ammonia and nitrite removal.…”

Section: Beta Diversity Analysismentioning

confidence: 91%

Integration of Marine Macroalgae (Chaetomorpha maxima) with a Moving Bed Bioreactor for Nutrient Removal from Maricultural Wastewater

Deng

et al. 2020

Archaea

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Rather than direct nutrient removal from wastewaters, an alternative approach aimed at nutrient recovery from aquacultural wastewaters could enable sustainable management for aquaculture production. This study demonstrated the feasibility of cultivating marine macroalgae (Chaetomorpha maxima) with a moving bed bioreactor (MBBR-MA), to remove nitrogen and phosphorus in aquaculture wastewater as well as to produce macroalgae biomass. MBBR-MA significantly increased the simultaneous removal of nitrate and phosphate in comparison with only MBBR, resulting in an average total nitrogen (TN) and total phosphorus (TP) removal efficiency of 42.8±5.5% and 83.7±7.7%, respectively, in MBBR-MA while MBBR had no capacity for TN and TP removal. No chemical oxygen demand (COD) removal was detected in both reactors. Phosphorus could be a limiting factor for nitrogen uptake when N : P ratio increased. The recovered nitrogen and phosphorus resulted in a specific growth rate of 3.86%–10.35%/day for C. maxima with an uptake N : P ratio of 6. The presence of macroalgae changed the microbial community in both the biofilter and water by decreasing the relative abundance of Proteobacteria and Nitrospirae and increasing the abundance of Bacteroidetes. These findings indicate that the integration of the macroalgae C. maxima with MBBR could represent an effective wastewater treatment option, especially for marine recirculating aquaculture systems.

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Influence of Partial Denitrification and Mixotrophic Growth of NOB on Microbial Distribution in Aerobic Granular Sludge

Cited by 62 publications

References 55 publications

Treatment of Liquid Phase of Digestate from Agricultural Biogas Plant in a System with Aerobic Granules and Ultrafiltration

Treatment of Liquid Phase of Digestate from Agricultural Biogas Plant in a System with Aerobic Granules and Ultrafiltration

Responses of bacterial community structure and denitrifying bacteria in biofilm to submerged macrophytes and nitrate

Integration of Marine Macroalgae (Chaetomorpha maxima) with a Moving Bed Bioreactor for Nutrient Removal from Maricultural Wastewater

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