Influence of particle size of hydroxyapatite as a capping agent on cell proliferation of cultured fibroblasts

In vitro and in vivo evaluation of the biocompatibility of a calcium phosphate/poly(lactic-co-glycolic acid) composite

“…Biphasic bioceramics (TCP and hydroxyapatite) are used to induce the formation of mineralized tissue and for bone reconstruction [19,20]. Our results agree with studies of the viability of human pulp fibroblasts cultured in the presence of hydroxyapatite powder [21] and hydroxyapatite particles [22]. Cytotoxicity was not observed on pulp fibroblasts cultured in contact with biphasic calcium phosphate/PLGA bioceramic [23], nor was it observed in dental cells cultured on biodegradable polymer scaffolds [24].…”

“…The mechanism of action after material placement is initially phagocytosis of the calcium phosphate particles, which causes them to biodegrade. In vitro, calcium phosphate materials were shown to promote collagen production and phenotypic expression of proteoglycans and matrix proteins associated with mineralization [22,39,40]. Studies in similar models with overnight incubations of cultured fibroblasts showed that the cells were able to adhere, spread, and remain viable on BCP/ PLGA composite discs, as evidenced by SEM [23].…”

The performance of human dental pulp stem cells on different three-dimensional scaffold materials

“…In dentistry, calcium phosphate-related materials are widely applied as pulpcapping agents for dentin-pulp complex regeneration, too [18,19]. Moreover, calcium phosphate ceramics have been used to manufacture dental implants [20,21].…”

Alveolar bone regeneration in rats after grafting of anorganic bovine bone and thick synthetic bioceramic

“…20,21,22 In the 15-day period, the inflammatory infiltrate appeared Groups Inflammatory infiltrate Fibroblast density Angioblastic density Bone neoformation T07 T15 T30 T07 T15 T30 T07 T15 T30 T07 T15 discreet such as in GI. This reduction is based on an initial aggressive reaction to a foreign body, which is later on attenuated due to the biocompatibility of the graft, 11,15,22,23 which may be attributed to the fact that both bone substitutes are composed of hydroxyapatite similar to the crystalline phase of natural bone. 24 It was also found that the presence of biomaterial inside the socket did not delay the healing process, thereby agreeing with the results of previous studies.…”