Influence of PEG generated osmotic stress on shoot regeneration and some biochemical parameters in Lathyrus culture

Piwowarczyk, Barbara; Kamińska, Iwona; Rybiński, W.

doi:10.17221/110/2013-cjgpb

Cited by 39 publications

(37 citation statements)

References 23 publications

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“…This larger reduction with PEG solution could be attributed to high viscosity, where solubility and diffusion of oxygen were reduced compared to control. At water potential -10.0 bars, the rate of germination of the rice genotypes was below 50% which is similar to the results obtained by [1] and [11]. This may also be the consequence of hampered water and nutrient absorption due to a decreasing water potential of the medium or greatly suppressed cell elongation as a result of the low turgor pressure.…”

Section: Effect Of Water Stress On Germination Percentagesupporting

confidence: 87%

“…Three genotypes namely, AURC 12 (G27), Koduveliyan (G45) and Onamuttan (G46) recorded moderate drought tolerance (-7.5 bars). Long roots was reported as a component trait for drought tolerance by [1] and [11] as they play a direct role with high penetration ability and have large xylem vessel radii and lower axial resistance to water flux aiding in greater water acquition.…”

Section: Effect Of Water Stress On Root Lengthmentioning

confidence: 99%

“…Early and rapid root elongation is an important indicator of drought tolerance and root length is more affected than shoot length under drought. Screenings for drought tolerance at seedling stage using osmotic solutions is reported as one of the robust methods to study resistance during germinal phase by [8] and [11]. With this background, the present study was undertaken to evaluate the drought tolerance of the 50 rice genotypes at seedling stages using polyethylene glycol 6000 (PEG 6000).…”

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In Vitro Screening of Indica Rice Genotypes for Drought Tolerance Using Polyethylene Glycol

2016

IJAAEE

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Abstract-Rice (Oryza sativa L 2n=24), one of the three major food crops of the world, covers 11 per cent of the arable land in the world. One of the main constraints of rice cultivation and production is drought, which affects the vegetative growth rate and grain yield. The present investigation was carried out to study the drought tolerance of 50 indica rice genotypes including 24 modern varieties, 16 rice cultures and ten traditional varieties along with the drought tolerant check varieties PMK 3 and MDU 5. The 50 rice genotypes were screened in in vitro bioassay method by using Polyethylene glycol (PEG 6000 MW) to assess their seedling response under drought. Drought stress was simulated at four different concentrations namely, -3.0, -5.0, -7.5 and -10.0 bars of water potential by dissolving 115, 196, 235 and 289 grams of PEG 6000 respectively in 1000 ml of distilled water. A control (0.0 bar) was maintained using distilled water The experiment was laid in completely randomized design with two replications. Data were recorded on germination percentage, root length, shoot length and root / shoot ratio at four different levels of treatment. Screening of the 50 rice genotypes for tolerance to drought at seedling stage using various concentrations of PEG 6000 resulted in the identification of nine rice genotypes namely, G3, G19, G21, G24, G25, G31, G35, G49 and G50 with tolerance to drought. The in vitro screening of rice seedlings using PEG 6000 can be adjudged as a simple, rapid and preliminary bioassay that can be used in mass screening for evaluating seedling of rice genotypes under drought.Index Terms-Drought, in vitro screening, PEG, Rice I. INTRODUCTION Drought is one of the major limiting factor that affects rice productivity in Asia [9]. It is estimated that by 2025, 15 million hectares of irrigated land and 22 million hectares in Asia will be under physical and economic water scarcity respectively [12].The percentage of drought affected land in the world has more than doubled since 1970s [4]. Early and rapid root elongation is an important indicator of drought tolerance and root length is more affected than shoot length under drought. Screenings for drought tolerance at seedling stage using osmotic solutions is reported as one of the robust methods to study resistance during germinal phase by [8] and [11]. With this background, the present study was undertaken to evaluate the drought tolerance of the 50 rice genotypes at seedling stages using polyethylene glycol 6000 (PEG 6000). II. MATERIALS AND METHODS A. Genetic materialsExperimental material comprised of fifty genotypes with broad genetic base involving 24 modern varieties, 16 rice cultures and ten traditional varieties along with the drought tolerant check varieties PMK 3 and MDU 5. B. Laboratory experimentThe experiment was conducted at the Abiotic Stress Laboratory of Department of Genetics and Plant Breeding, Faculty of Agriculture during August, 2014. The in vitro screening for drought tolerance was carried out using Polyethylene Glycol (PEG ...

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Section: Effect Of Water Stress On Germination Percentagesupporting

confidence: 87%

Section: Effect Of Water Stress On Root Lengthmentioning

confidence: 99%

mentioning

confidence: 99%

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In Vitro Screening of Indica Rice Genotypes for Drought Tolerance Using Polyethylene Glycol

2016

IJAAEE

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“…Grass pea (Lathyrus sativus L.) is a legume species resistant to harsh growing conditions, such as drought, salinity or periodic flooding (Piwowarczyk et al 2014(Piwowarczyk et al , 2016(Piwowarczyk et al , 2017. Due to the advantageous composition of seeds, high level (approx.…”

Section: Introductionmentioning

confidence: 99%

Response of grass pea (Lathyrus sativus L.) photosynthetic apparatus to short-term intensive UV-A:red radiation

et al. 2019

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Plants growing under natural conditions are constantly exposed to ultraviolet (UV), primarily UV-A, radiation. Grass pea (Lathyrus sativus L.) is a legume species resistant to harsh growing conditions, such as drought, salinity or periodic flooding. Due to the advantageous composition of seeds, it is used for consumption in such regions as South Asia or East Africa where high intensity of UV radiation occurs. Absorption of this spectral range causes changes in the photosynthetic apparatus of plants, including damage to the photosystem II (PSII) reaction centres. The aim of the work was to examine whether the use of the combination UV-A: red light as a source of radiation would enable quick acclimatization of the photosynthetic apparatus of grass pea to the negative effect of UV-A radiation. 14-day-old plants were exposed to UV-A:red radiation for 48 h. The plants exposed to UV-A:red radiation showed enhanced effective efficiency of PSII and increased total electron carriers, which enabled more effective photosynthesis at higher values of radiation intensity in comparison with control plants, kept under white LED light. At the same time, there were no statistically significant differences in both the photosynthetic pigment contents and the level of lipid peroxidation. The obtained results indicate that the observed increase in the efficiency of CO 2 carboxylation after short-term UV-A:red radiation has resulted from the efficient linear electron transport due to maintaining the effective oxygen evolving complex (OEC) and increased total electron carriers.

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“…Grass pea (Lathyrus sativus, Fabaceae) is a crop highly tolerant to the stress caused by various abiotic factors (Vaz Patto et al 2006). Even though the exact mechanism of this tolerance is still not known, there are reports indicating that it can rely on the ability to adjust the plants osmotic potential (Jiang et al 2013;Piwowarczyk et al 2014), elevated antioxidant enzyme activity (Jiang et al 2013), accumulation of polyamines and b-N-oxalyl-L-a,b-diaminopropionic acid (Xing et al 2001;Xiong et al 2006).…”

Section: Introductionmentioning

confidence: 99%

Different acclimatization mechanisms of two grass pea cultivars to osmotic stress in in vitro culture

et al. 2017

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Grass pea (Lathyrus sativus L.) is a legume crop known from its tolerance to various abiotic stresses, especially drought. In this study, we investigated: (1) the response of grass pea seedlings to osmotic stress generated in vitro by polyethylene glycol (PEG); (2) potential drought acclimatization mechanisms of two polish grass pea cultivars. Grass pea seeds of two cultivars were sown on media containing different PEG concentrations (0, 5.5, 11.0 mM) and cultivated for 14 days in controlled conditions. Plants' dry matter increased under osmotic stress (regardless of PEG concentration). In turn, the highest dose of PEG caused a reduction in seedling growth in both cultivars. Furthermore, PEG caused the peroxidase activity increase in whole seedlings and catalase (CAT) activity in roots. However, differences between cultivars were noted in: CAT activity in shoots; while phenols and anthocyanin content as well as electrolyte leakage in shoots and roots.In turn, in both tested genotypes, accumulation of proline increased in shoots under osmotic stress. Obtained results indicate that the examined plants, although belonging to the same species, differ in acclimatization processes leading to elevated tolerance to osmotic stress.

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Influence of PEG generated osmotic stress on shoot regeneration and some biochemical parameters in Lathyrus culture

Cited by 39 publications

References 23 publications

In Vitro Screening of Indica Rice Genotypes for Drought Tolerance Using Polyethylene Glycol

In Vitro Screening of Indica Rice Genotypes for Drought Tolerance Using Polyethylene Glycol

Response of grass pea (Lathyrus sativus L.) photosynthetic apparatus to short-term intensive UV-A:red radiation

Different acclimatization mechanisms of two grass pea cultivars to osmotic stress in in vitro culture

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