Influence of photoperiod on the localization of Δ5-3β-hydroxysteroid dehydrogenase in the ovaries of maturing Japanese quail

Activity of ovarian 5-3 -hydroxysteroid dehydrogenase in the domestic fowl (Gallus domesticus) with respect to age

“…Ovarian steroidogenesis in birds starts during early embryogenesis (Gilbert, 1971;Lofts & Murtón, 1973;Murton & Westwood, 1977) and persists into adult life; constant positive formazan reaction of stromal and thecal interstitial ceUs of ovaries of aU ages examined by the histochemical reaction can be considered to reflect this activity. However, in contrast to observations in the quail (Sayler, Dowd & Wolfson, 1970) and duck (Deray & Gomot, 1978), no change was found histochemically in the activity of the interstitial cells with age. This inability to detect histochemical changes in 3 ß-HSD in these cells concomitant with physical changes, e.g.…”

“…The lack of a positive reaction in the granulosa cells of smaU folUcles is at variance with observations in the quail (Sayler et al, 1970). The occurrence of positive granulosa cells in maturing follicles of hens is not unexpected, but previous work has not emphasized the change in the activity of the granulosa cells with time as described here, a factor which may have a significant implication in the reproductive activity of the hen.…”

Immunohistochemical Study of 3 β‐Hydroxysteroid Dehydrogenase in Dog’s Perianal Sinus

“…The present study shows for the first time immunopositivity for 3β‐HSD in canine perianal sinuses. The observed positive reaction in cells, located in the propria of the sinus is similar to that of the interstitial ovarian cells, observed by a number of authors (Davenport and Mallete, 1966; Davies et al., 1966; Boucek and Savar, 1970; Sayler et al., 1970). These investigators believe that the increased enzyme activity was proportional to the growth of the ovary and the increasing age, we assume that possibly, 3β‐HSD plays a role in the development of PS.…”

“…In men and rhesus monkeys, 3β‐HSD activity was established in the adrenal cortex (Sholl, 1983; Goto et al., 2006), the testis (Sholl, 1983), the ovary (Davies et al., 1966) the placenta (Sholl, 1983), the skin (Zhao et al., 1991; Gingras and Simard, 1999; Simard et al, 1993), the mammary gland (Zhao et al., 1991) etc. The localization of enzymes have been evidenced in the stroma and the functional tissue in the ovary (Davenport and Mallete, 1966; Boucek and Savar,1970), the testis (Davies et al., 1966), the placenta (Davies et al., 1966) and the brain (Soma et al., 2004) in a number of animal and avian species: rat (Davies et al., 1966), rabbit (Davenport and Mallete, 1966), cow (Davies et al., 1966), zebra (Soma et al., 2004), guinea pig (Davies et al., 1966), Japanese quail (Sayler et al., 1970), domestic chicken (Boucek and Savar, 1970).…”

Seasonal fluctuation of cholesterol in ovarian compartments of birds

“…; Lofts and Murton, 1973 ;Guraya, 1976a). To fill this gap, the present study was initiated to determine the seasonal morphological, histochemical (Guraya and Chalana, 1976 ;Chalana and Guraya, 1977 (Taber, 1951 ;Marshall and Coombs, 1957 ;Woods and Domm, 1966 ;Saylor et al,1970 ;Kern, 1972 ;Lofts and Murton, 1973 ;Guraya, 1976a, b). Therefore, it is reasonable to conclude that the cholesterol depletion in the interstitial cells during the breeding periods of the crow, myna and house sparrow must be closely related to the increasing secretion of androgens required for heightened breeding activities (Lofts, 1975 …”