Influence of platelet-rich plasma (PRP) on osteogenic differentiation of rat bone marrow stromal cells. An in vitro study

Arpornmaeklong, Premjit; Kochel, Michael; Depprich, Rita; Kübler, Norbert R.; Würzler, K. K.

doi:10.1054/ijom.2003.0492

Cited by 185 publications

(159 citation statements)

References 47 publications

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“…Under these circumstances, our aim in this animal model was to influence the osseointegration process with the use of PRP. Previous studies have reported controversial results regarding the effectiveness of PRP in bone healing [15,[31][32][33], but none was supported by level I clinical evidence. Consistently, in the two time periods in our study, there were no histological differences in osseointegration that were shown to be responsible for failures [8].…”

Section: Discussionmentioning

confidence: 96%

The effect of platelet-rich plasma on osteochondral defects treated with mosaicplasty

Altan

Aydın

Erkoçak

et al. 2014

International Orthopaedics (SICOT)

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Purpose This study investigated the efficacy of platelet-rich plasma (PRP) on articular surfaces on which the mosaicplasty technique was performed. Our hypothesis was that PRP can accelerate the osseointegration process and enhance the quality of articular integrity after the mosaicplasty procedure. Methods Standard defects were created in the femoral groove of both patellofemoral joints of 12 New Zealand rabbits. PRP solution was placed inside the defect before fixation of the osteochondral autografts and injected inside the involved joint after capsular closure of the tested knees. The contralateral knees served as the control sides. The animals were euthanized three or six weeks after mosaicplasty, and both limbs were assessed according to Pineda's histological grading scale. Significance level was set at p≤0.05 a priori, and the Mann-Whitney U test was used for statistical analysis. Results Histologic findings at the interface between the transferred autograft and the original cartilage revealed better integration of the adjacent surfaces in the mosaicplasty with PRP group three weeks after the procedure; the difference was significant (p<0.05). However, no significant difference in the transition zone was observed between the groups six weeks after the experiment (p=0.59). Conclusions Our animal model showed that adjunctive use of PRP produced a better healing response and resulted in superior histological scores after three weeks compared with the mosaicplasty-only procedure. Interpretation of our results is important in terms of rapid return to previous activity levels. Thus, application of PRP can represent a valid therapeutic option for improving the efficacy of mosaicplasty by stimulating the local healing response.

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Section: Discussionmentioning

confidence: 96%

The effect of platelet-rich plasma on osteochondral defects treated with mosaicplasty

Altan

Aydın

Erkoçak

et al. 2014

International Orthopaedics (SICOT)

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“…Arpormaeklong et al 13 (rat bone marrow stromal cells, rhBMP2, PPP = platelet-poor plasma) demonstrated the PRP as estimulator of the cellular proliferation (dose-dependent), and inhibitor of the activity of alkaline phosphatase and calcium deposition. Choi et al 37 (alveolar bone cells, PPP, PC = platelets concentrate without plasma) observed that PRP in high concentrations suppress the viability and the cellular proliferation, however there is a stimulation effect in low concentrations (1-5%).…”

Section: Discussionmentioning

confidence: 99%

“…Although there is still no consensus about the ideal platelet concentration that could optimize the tissue repair process, some in vivo and in vitro studies suggest that a PRP highly concentrated could even be harmful to the repair [13][14][15] .…”

Section: Introductionmentioning

confidence: 99%

Effects of a highly concentrated platelet-rich plasma on the bone repair using non-critical defects in the calvaria of rabbits

et al. 2010

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PURPOSE: To verify the effect of highly concentrated platelet-rich plasma (hPRP) in the pathways of bone repair using non-critical defects in the calvaria of rabbits. METHODS: The hPRP was produced from collected venous blood of 21 rabbits. Four non-critical defects of 8 mm in diameter were created on the calvaria of each animal. The defects were all treated differently: autogenous particled bone (APB, group 1), autogenous particled bone associated with hPRP (APB + hPRP, group 2), isolated hPRP (group 3), and blood clot (control, group 4). Animals were submitted to euthanasia on the 2nd, 4th and 6th week postoperatively. Histological and histomorphometric analysis were carried through. RESULTS: After two weeks, groups 1 and 2 were in more advanced stage of repair than 3 and 4. At this period, comparing the groups 1 and 2, no significant differences were found between both, which also happened between the groups 3 and 4. However, after four and six weeks, the group 1 showed a more advanced stage of repair among all the other studied groups, while group 2 was in more advanced signs of bone repair than groups 3 and 4. Comparing groups 3 and 4, after four and six weeks, the least advanced stage of bone repair was found to be within group 3. CONCLUSION: The use of a highly concentrated PRP was considered prejudicial to the repair of non-critical defects in the calvaria of rabbits, either in the association of autogenous particled bone, when compared to autogenous particled bone alone, or in its isolated form, when compared to blood clot (control).

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“…The influential time of the growth factors of the PRP is up to 6-7 months. 13 In our study, the consolidation of the graft was earlier in Group B patients in whom the ABS was mixed with PRP which was based on the premise that the large number of platelets in PRP release significant quantities of growth factors that aid bone graft maturation in accordance with Consolo et al, 13 Marx, 14 Green David and Klink 15 and Messora et al, 16 but contradictory to Shanaman et al, 17 Arpornmaeklong et al, 18 de Vasconelos et al 19 and enhances bone regeneration. Advantages of using an autologous PRP include no risk of cross-reactivity, immune reaction or disease transmission.…”

Section: Discussionmentioning

confidence: 62%

Role of platelet-rich plasma in combination with alloplastic bone substitute in regeneration of osseous defects

Singh¹,

Gupta²,

Pradhan³

et al. 2011

Journal of Oral Biology and Craniofacial Research

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Introduction: Bone grafts are frequently used for the treatment of bone defects, but can cause postoperative complications, and sometimes a sufficient quantity of bone is not available. Hence, synthetic biomaterials have been used as an alternative to autogenous bone grafts. Recent clinical reports suggest that application of autologous blood plasma enriched with platelets can enhance the formation of new bone. There are very few in vitro or in vivo studies published on the efficiency of platelet-rich plasma (PRP). The objective of this study was to evaluate the alloplastic bone substitute for its osteogenic potential with or without PRP.

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Influence of platelet-rich plasma (PRP) on osteogenic differentiation of rat bone marrow stromal cells. An in vitro study

Cited by 185 publications

References 47 publications

The effect of platelet-rich plasma on osteochondral defects treated with mosaicplasty

The effect of platelet-rich plasma on osteochondral defects treated with mosaicplasty

Effects of a highly concentrated platelet-rich plasma on the bone repair using non-critical defects in the calvaria of rabbits

Role of platelet-rich plasma in combination with alloplastic bone substitute in regeneration of osseous defects

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