Influence of Pleurotus ostreatus inoculation on wood degradation and fungal colonization

Piškur, Barbara; Bajc, Marko; Robek, Robert; Humar, Miha; Sinjur, Iztok; Kadunc, Aleš; Oven, Primož; Rep, Gregor; Petkovšek, Samar Al Sayegh; Kraigher, Hojka; Jurc, Dušan; Pohleven, Franc

doi:10.1016/j.biortech.2011.09.008

Cited by 15 publications

(12 citation statements)

References 24 publications

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“…For isolation of DNA from plant and fungal tissues, as well as wood samples, DNeasy Plant Mini kit (Qiagen) was reported as extremely efficient in yielding inhibitorfree DNA of good integrity and quantity (Eikenes et al 2005). When it comes to the isolation of total DNA from soil samples, PowerSoil® DNA isolation kit (Mo Bio Laboratories, inc.) had proven itself as superior in terms of DNA quality and isolation reproducibility (Mahmoudi et al, 2011) and was also reported as very efficient in isolation of fungal DNA from decomposing wood samples (Piškur et al, 2011).…”

Section: Resultsmentioning

confidence: 99%

Vpliv glivnih in rastlinskih sekundarnih metabolitov na verižno reakcijo s polimerazo (PCR)

Thaler¹,

Bajc²

2013

Acta Silvae et Ligni

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25 UVOD INTRODUCTIONSekundarni metaboliti so vse tiste organske spojine, ki niso neposredno vpletene v normalno rast, razvoj in razmnoževanje organizma (Fraenkel, 1959). Pogosto imajo izjemne fiziološke aktivnosti (Keller in sod., 2005), nimajo pa splošno prepoznane neposredne vloge v procesih fotosinteze, dihanja, transporta raztopin, premestitev, sinteze beljakovin, asimilacije hranil, diferenciacije ali izgradnje ogljikovih hidratov in lipidov (Teiz in Zeiger, 2002). V rastlinah so veči-noma v vlogi obrambnih (pred rastlinojedi, mikrobi, IZVLEČEKSekundarni metaboliti so organske spojine, ki jih najdemo pri glivah in rastlinah, kjer imajo vlogo obrambnih in signalnih molekul ali zagotavljajo druge selekcijske prednosti, niso pa neposredno vpleteni v rast, razvoj in razmnoževanje organizma. Pri delu s tehnikami DNA so pogosto ravno sekundarni metaboliti tisti, ki posredno ali neposredno vplivajo na uspešnost verižne reakcije s polimerazo (PCR) ali reverezno transkriptazo, in sicer tako, da otežujejo celično lizo, povzročajo razpad nukleinskih kislin ali neposredno ovirajo delovanje encima polimeraze pri pomnoževanju tarčne DNK. Glavna ovira pri aplikaciji tehnike PCR v rutinski diagnostiki je priprava visokokvalitetne DNA brez inhibitorjev. Še posebej to velja pri izolaciji DNA iz lesnatih rastlin (Minafra in sod., 1992) in vzorcev tal (Tsai in Olson, 1991). Večina standardnih postopkov izolacije nukleinskih kislin ne odstrani rastlinskih polisaharidov in polifenolnih komponent, ki imajo lahko neposreden vpliv na pomnoževanje s PCR (Demeke in Adams, 1992). Poskusi za premostitev tovrstnih ovir vključujejo bolj dovršene metode za izolacijo nukleinskih kislin in PCR, ki vključujejo uporabo pospeševalcev PCR za odstranitev ali zmanjšanje vpliva inhibitorjev PCR. Ta pregled je osredotočen na pristope za odstranitev ali zmanjšanje vplivov rastlinskih in glivnih sekundarnih metabolitov iz vzorcev tal, različnih rastlinskih tkiv in razkrojenega lesa zaradi pomena tovrstnih raziskav za gozdarstvo in lesarstvo.Ključne besede: sekundarni metaboliti, izolacija DNA, pomnoževanje DNA, verižna reakcija s polimerazo (PCR), inhibicija PCR, pospeševalci PCR ABSTRACTSecondary metabolites are organic compounds that can be found in both fungi and plants, where they play an important role as defensive and signal molecules, or provide other kinds of advantage in natural selection, but are not directly involved in normal growth, development and reproduction of an organism. When working with DNA techniques, it is the secondary metabolites that most often affect the efficiency of polymerase chain reaction (PCR), either by hindering cell lysis, causing decomposition of nucleic acids or by direct inhibition of DNA-polymerase or reverse transcriptase amplification. The main limiting factor in the application of the PCR technique in routine diagnosis is the preparation of good quality nucleic acids isolates, free of PCR inhibitors. This is especially true in the case of woody plants (Minafra et al., 1992) and soil samples (Tsai and Ols...

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Section: Resultsmentioning

confidence: 99%

Vpliv glivnih in rastlinskih sekundarnih metabolitov na verižno reakcijo s polimerazo (PCR)

Thaler¹,

Bajc²

2013

Acta Silvae et Ligni

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“…Saprotrophic fungi are primary recyclers that break down surface detritus on the forest floor, where it then becomes accessible to other organisms (Arnstadt et al 2016;Marcot 2017a). The process of wood decomposition contributes to soil development and productivity, nutrient cycling, moisture retention, and forest regeneration (Bednarz et al 2013;Lonsdale et al 2008;Marcot 2017b;Pavlík et al 2008;Piškur et al 2011). Turkey tail (Trametes versicolor Pilat) is a saprotrophic fungus with a circumpolar distribution (Lepp 2012; Stamets 2000; Veena and Pandey 2012).…”

Section: Introductionmentioning

confidence: 99%

Turning an invasive hardwood into an asset: Inoculating Ligustrum lucidum logs with a medicinal mushroom, Trametes versicolor, accelerates wood decomposition under field conditions

O’Donnell¹,

Moulton²,

Stack³

2019

Invasive plant sci. manag.

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Glossy privet (Ligustrum lucidum W. T. Aiton) is a highly aggressive tree that has become globally invasive in a wide range of habitats and can quickly form dense thickets, shading and outcompeting native vegetation. Slowly decomposing slash following removal of dense infestations can create additional management challenges, including fire risk concerns, which curtailed planned invasive plant removal projects on certain tracts within the Balcones Canyonlands Preserve near Austin, TX. This prompted a pilot study to explore whether wood-decaying fungi could be used to hasten the recycling of L. lucidum logs back into the forest ecosystem. We inoculated 25 freshly cut L. lucidum logs with Trametes versicolor (Fr.) Pilat at two study sites and monitored the inoculated logs and 5 untreated control logs over a 3-yr period (February 2015 to March 2018). We found that inoculation significantly accelerated wood decay. By the end of our study, 100% of logs with >3 inoculation points were in advanced stages of decay, while only one of the control logs showed noticeable signs of decay. Inoculating logs in the field was easy and suitable for novices, requiring little or no previous experience to achieve successful results. An added benefit of using T. versicolor is its medicinal properties and potential for bioremediation. We have continued to inoculate logs with comparable success and are integrating them into forest restoration projects. Applied on a larger scale, these wood-decaying fungi have the potential to transform nonnative deadwood from a threat to an asset.

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“…However, the development of DNA-based PCR (Polymerase Chain Reaction) and taxon-specific primers has provided a range of new possibilities. For example, Piskur et al (2011) used PCR-DGGE method to analyze the fungal communities in degraded wood chips. Quantitative real-time PCR (qPCR) has proven to be a useful tool for the detection of plant pathogenic fungi and bacteria ( Hietala et al, 2009 ; Salm & Geider, 2004 ; Schaad & Frederick, 2002 ; Schena, Hughes & Cooke, 2006 ; Vandroemme et al, 2008 ), estimation of fungal biomass in forest soil ( Baldrian et al, 2013 ) but also wood deteriorating fungi ( Eikenes et al, 2005 ; Pilgård et al, 2011 ; Pilgård, Alfredsen & Hietala, 2010 ; Song et al, 2014 ).…”

Section: Introductionmentioning

confidence: 99%

DNA quantification of basidiomycetous fungi during storage of logging residues

Børja¹,

Alfredsen²,

Filbakk³

et al. 2015

PeerJ

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The demand for bioenergy caused an increased use of logging residues, branches and treetops that were previously left on the ground after harvesting. Residues are stored outdoors in piles and it is unclear to what extent fungi transform this material. Our objective was to quantify the amount of wood degrading fungi during storage using quantitative real-time PCR (qPCR) to detect basidiomycetous DNA in logging residues, a novel approach in this field. We found that the qPCR method was accurate in quantifying the fungal DNA during storage. As the moisture content of the piled logging residues decreased during the storage period, the fungal DNA content also decreased. Scots pine residues contained more fungal DNA than residues from Norway spruce. Loose piles had generally more fungal DNA than bundled ones.

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Influence of Pleurotus ostreatus inoculation on wood degradation and fungal colonization

Cited by 15 publications

References 24 publications

Vpliv glivnih in rastlinskih sekundarnih metabolitov na verižno reakcijo s polimerazo (PCR)

Vpliv glivnih in rastlinskih sekundarnih metabolitov na verižno reakcijo s polimerazo (PCR)

Turning an invasive hardwood into an asset: Inoculating Ligustrum lucidum logs with a medicinal mushroom, Trametes versicolor, accelerates wood decomposition under field conditions

DNA quantification of basidiomycetous fungi during storage of logging residues

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