Influence of polydopamine-mediated surface modification on oxygen-release capacity of haemoglobin-based oxygen carriers

Abstract

Oxidative toxicity has impeded the development of haemoglobin-based oxygen carriers (HBOCs) by causing methaemoglobin (MetHb) formation and inducing oxidative stress. In our previous work, polydopamine-coated haemoglobin (Hb-PDA) nanoparticles have been designed and synthesized with the capacity to reduce oxidative toxicity. In this investigation, the mass ratio of dopamine (DA) to haemoglobin (Hb) and the pH value are found to be the primary factors that influence preparation of Hb-PDA nanoparticles. X-ray photoelectron spectroscopy showed that the catechol groups of DA play a crucial role in the modification of Hb surface. Hb-PDA nanoparticles were found to exhibit oxidative protection from hydrogen peroxide (HO) and the change of mitochondrial membrane potential showed that the Hb-PDA nanoparticles reduced HO-induced apoptosis. It is demonstrated that modification of PDA could maintain the oxygen-release capacity of Hb. These findings confirm that Hb-PDA nanoparticles possess restrained oxidative toxicity and preserve oxygen-release capacity.

Synthesis of Nanoparticles Fully Made of Hemoglobin with Antioxidant Properties: A Step toward the Creation of Successful Oxygen Carriers

“…48 While several groups have reported the ability of PDA-coated HBOCs to scavenge reactive oxygen species (ROS), only our group and the Zhou group have been able to demonstrate that the antioxidant character of PDA can minimize the oxidation of Hb to metHb. 15,18,29,49,50 This is an important aspect since, apart from not being able to deliver oxygen, the oxidation of Hb to metHb results in dysregulated vascular tone and inflammatory reactions. 4 The PDA coating was fabricated by incubating the Hb-NPs in DA solutions for 30 and 60 min.…”

“…Figure 4A shows how increasing DA concentrations results in a decrease in ζ-potential with the lowest value (∼24.5 ± 0.4 mV) obtained for a DA concentration of 1.0 mg mL −1 . This decrease in ζ-potential following PDA coating has been shown in previous reports 49 and could be due to the covering of the surface chemical groups of Hb (e.g., amino groups from the lysine residues) by PDA. Interestingly, both studied reaction times gave rise to the similar ζ-potential values suggesting that 30 min already promoted the highest PDA deposition.…”

Hemoglobin-Based Oxygen Carriers Incorporating Nanozymes for the Depletion of Reactive Oxygen Species

“…Specifically, individual Hb molecules have been PDAcoated, but also larger particles fully made of Hb. [33][34][35][36] Herein, Hb's coating by PDA was conducted employing a weight ratio 5:1 of Hb and DA for 3 h.…”

The P 50 value detected by the oxygenation-dissociation analyser and blood gas analyser

“…A 4-ml sample of blood was placed in a heparin-coated anticoagulation tube (Kangjian Medical Apparatus, Jiangsu, China), while 50 ml of blood was mixed with a solution of citrate phosphate dextrose adenine (CPDA-1; Sigma Aldrich, St Louis, MO, USA). All samples were stored at 4 C. Bovine whole blood was withdrawn via the carotid vein of cattle (Beijing Created Biotechnology, Beijing, China), mixed with CPDA-1, and stored at 4 C. Next, human haemoglobin (hHb) and bovine haemoglobin (bHb) were purified from whole blood mixed with CPDA-1 by anion-exchange chromatography, as previously described [28][29][30]. Haemoglobin solutions were prepared at a concentration of 5 g/dL.…”