Influence of polymer structure and biodegradation on DNA release from silk–elastinlike protein polymer hydrogels

Hwang, David G.; Moolchandani, Vikas; Dandu, Ramesh; Haider, Mohamed; Cappello, Joseph; Ghandehari, Hamidreza

doi:10.1016/j.ijpharm.2008.10.021

Cited by 40 publications

(43 citation statements)

References 19 publications

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“…Different delivery strategies have been investigated using these SELPs for efficient delivery of therapeutic proteins and peptides. [265][266][267][268][269][270] The hydrogel network formed by SELP controls the release of incorporated therapeutic proteins from hours to days depending upon the concentration of SELP, charge on the released agents and ionic strength of the release media. 268,271,272 significance.…”

Section: Poly(lactic-co-glycolic Acid)mentioning

confidence: 99%

“…[265][266][267][268][269][270] The hydrogel network formed by SELP controls the release of incorporated therapeutic proteins from hours to days depending upon the concentration of SELP, charge on the released agents and ionic strength of the release media. 268,271,272 significance. 6,[273][274][275] Many therapeutic proteins incorporated in polymers have also been approved from FDA for the treatment of different diseases and syndromes.…”

Section: Poly(lactic-co-glycolic Acid)mentioning

confidence: 99%

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Natural and Synthetic Polymers as Drug Carriers for Delivery of Therapeutic Proteins

2015

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In order to cure and treat health-related disorders, therapeutic substance must reach its target site with a constant concentration over a long period of time. As oral administration is limited due to enzymatic degradation, most of the commercially available therapeutic proteins are usually being administered parenterally. However, because of their short biological half-life, daily multiple injections are required to maintain effective therapeutic levels of these drug candidates. To limit this drawback, a variety of polymers are being used to increase systemic bioavailability of therapeutic proteins and peptides. Development of protein-based therapeutic substances has tremendously increased the need for suitable polymeric-based carrier systems, guaranteeing safe and sustained delivery of therapeutic proteins to their target site. Here, we have briefly discussed two major types of polymers including natural and synthetic polymers that have been intensively studied for efficient delivery of various proteinous drugs. A wide variety of natural and/or synthetic polymers have been found to be useful and safe drug carrier systems for the delivery of therapeutic proteins which have been discussed over here in detail. To conclude, these polymers have been

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Section: Poly(lactic-co-glycolic Acid)mentioning

confidence: 99%

Section: Poly(lactic-co-glycolic Acid)mentioning

confidence: 99%

Natural and Synthetic Polymers as Drug Carriers for Delivery of Therapeutic Proteins

2015

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“…Tight control over the primary sequence of these polymers afforded by recombinant DNA technology was vitally important to effectively determine the contribution of each length and ratio of silk and elastin to final polymer properties. It was found that altering the composition ratios and sequence had significant effects on soluble fraction, [102, 104, 111], swelling ratio, [101, 102, 111, 112] and mechanical strength[101, 102, 113] of resulting hydrogels. These findings lead to the ability to tune release of bioactive agents based on size and surface characteristics.…”

Section: Silk-elastinlike Protein Polymersmentioning

confidence: 99%

Controlled release from recombinant polymers

Price

Poursaid

Ghandehari

2014

Journal of Controlled Release

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Recombinant polymers provide a high degree of molecular definition for correlating structure with function in controlled release. The wide array of amino acids available as building blocks for these materials lend many advantages including biorecognition, biodegradability, potential biocompatibility, and control over mechanical properties among other attributes. Genetic engineering and DNA manipulation techniques enable the optimization of structure for precise control over spatial and temporal release. Unlike the majority of chemical synthetic strategies used, recombinant DNA technology has allowed for the production of monodisperse polymers with specifically defined sequences. Several classes of recombinant polymers have been used for controlled drug delivery. These include, but are not limited to, elastin-like, silk-like, and silk-elastinlike proteins, as well as emerging cationic polymers for gene delivery. In this article, progress and prospects of recombinant polymers used in controlled release will be reviewed.

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“…The development of cell-mediated release rates is ideal for tissue engineering applications, allowing for both spatial and temporal control over gene transfection based on cellular demand [155]. In addition, alterations to the polymer structure, ionic strength of media during encapsulation, and gelation time, as demonstrated using silk-elastinlike polypeptide (SELPs), influence the loading capacity and total release of naked plasmid DNA from hydrogels [156]. These results show that the development of dynamic and robust delivery platforms depends on being able to control the total loading capacity as well as the release rate of bioactive factors.…”

Section: Delivery Of Soluble Cell Signaling Moleculesmentioning

confidence: 99%

Hydrogels from Protein Engineering

Greenwood‐Goodwin

Heilshorn

2012

Biomimetic Approaches for Biomaterials Development

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Protein-engineered hydrogels build upon the natural structure and function of proteins to create well-defined, multifunctional materials by cross-linking polypeptide chains together. Individual polypeptide chains are composed of independent peptide domains built from amino acid monomers. The engineered amino acid sequence is designed to form specific and reproducible structures of the selected peptide domains. The exact amino acid sequence of the polypeptide chain can be encoded into a DNA sequence, which is transfected into a host microorganism in order to produce a monodisperse batch of polypeptide chains. Interactions between individual polypeptide chains can be engineered to form hydrated macromolecular structures with tunable mechanical strength. These interchain interactions can include both noncovalent cross-linking between neighboring peptide domains as well as covalent cross-linking between amino acid residues to form stable hydrogels.In this chapter, we discuss the development of protein-engineered hydrogels with tunable mechanical and biochemical properties for biomaterial applications. First, we discuss the fundamentals of protein structure and recombinant protein synthesis. Next, we review the diversity of peptide domains that have been utilized in protein-engineered hydrogels to date, including cell-binding domains, structural domains, and molecular recognition domains. We continue to describe the various enzymatic and synthetic chemistry strategies that have been utilized to create covalently cross-linked protein-engineered hydrogels. We conclude with an in-depth discussion of cellular interactions with protein-engineered hydrogels and of the ability to tune the biomechanics and biochemistry of the materials to direct cellular phenotype.Development of novel protein-engineered hydrogels for therapeutic applications requires regulation of specific cellular behavior through tuning of the structural, mechanical, and biochemical properties of the hydrogel. The cellular response and activation of intracellular signaling pathways for cell adhesion and migration is different between traditional two-dimensional (2D) cell culture environments and native three-dimensional (3D) cell environments, thus the use of 3D materials for Biomimetic Approaches for Biomaterials Development, First Edition. Edited by João F. Mano.

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Influence of polymer structure and biodegradation on DNA release from silk–elastinlike protein polymer hydrogels

Cited by 40 publications

References 19 publications

Natural and Synthetic Polymers as Drug Carriers for Delivery of Therapeutic Proteins

Natural and Synthetic Polymers as Drug Carriers for Delivery of Therapeutic Proteins

Controlled release from recombinant polymers

Hydrogels from Protein Engineering

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