Influence of Polysaccharides From Polygonatum kingianum on Short-Chain Fatty Acid Production and Quorum Sensing in Lactobacillus faecis

Abstract

Polysaccharide is one of the main active ingredients of Polygonatum kingianum, which has been proven to regulate the balance of gut microbiota. For the first time, this study focused on the regulation of polysaccharides from Polygonatum kingianum (PS) on Lactobacillus faecis, a specific probiotic in the intestinal tract. PS effectively promoted the biomass, biofilm and acetic acid production in L. faecis 2-84, and enhanced quorum sensing (QS) signaling. The characteristics of gene sequence were analyzed using genomics approaches, and L. faecis 2-84 was found to encode 18 genes that are closely related to QS and 10 genes related to short-chain fatty acids (SCFAs). Additionally, transcriptome and proteome analysis demonstrated that PS could promote the QS system of L. faecis by enhancing the transcription of oppA gene and expression of oppD protein. PS also regulated the production and metabolism of SCFAs of L. faecis by upregulating the expression of ldh and metE gene and adh2 protein, and downregulating the expression of mvK gene. In conclusion, it was speculated that PS could affect intestinal SCFAs production by affecting the QS system and SCFAs production in L. faecis. The present study implied that PS might have a role in promoting the growth of intestinal probiotics, where the QS system and SCFAs might be two of the important mechanisms for the probiotic activity of PS.

Jiedu-Yizhi Formula Alleviates Neuroinflammation in AD Rats by Modulating the Gut Microbiota

“…s_Lactobacillus_faecis is a lactic acid producer, and studies on this bacterium are limited. However, s_Lactobacillus_faecis is closely related to s_Lactobacillus murine based on gene sequence phylogenetic analysis [ 79 , 80 ], and s_Lactobacillus murine mediates the release of IL-10 by TLR2 receptors to restrict the inflammatory response [ 81 ]. Thus, s_Lactobacillus_faecis may represent a potential AD-modifying probiotic.…”

Health-Promoting Activities and Associated Mechanisms of Polygonati Rhizoma Polysaccharides

“…PSP could effectively promote the biomass, biofilm, and acetic acid production in Lactobacillus faecis , a specific probiotic in the intestinal tract [ 119 ]. Particularly, PSP promoted the quorum sensing system of L. faecis through enhancing the transcription of oppA and expression of oppD protein [ 119 ]. The proliferation of beneficial microbiotas, containing Parabacteroides and Bifidobacterium, was positively associated with PSP treatment [ 120 ].…”

Making Sense of Quorum Sensing at the Intestinal Mucosal Interface

“…Gastric acid reduces the pH in the stomach to about 2–5, which constitutes a significant inhibitory barrier for most bacteria. Distal of the stomach, Lactobacillus and Bifidobacterium species can produce lactic acid and short chain fatty acids, respectively, which contribute to pH regulation of the intestine [ 67 , 68 , 69 ]. Acidic pH activates QS in Lactobacillus rhamnosus for example, and this is associated with increased bacteriocin production [ 70 , 71 , 72 , 73 , 74 ].…”