Influence of presumptive reagents on DNA typing

Efficiency of DNA extraction from urine test strips

“…Extraction was performed using EZ1 DNA Investigator Kit with EZ1 Advanced XL (QIAGEN GmbH, Hilden, Germany), and elution was carried out with 50 ml of TE. DNA quantification was performed with Applied Biosystems 7500 RealTime PCR System (Life Technologies/Thermo Fisher Scientific, South San Francisco, CA, USA) [2], PCR amplification with AmpFlSTR Identifiler Plus PCR Amplification Kit, and electrophoresis with the Applied Biosystems 3130xl genetic analyzer. Signal intensities exceeding 150 RFU were regarded as valid.…”

DNA extraction with silica membrane column from teeth left in soil for more than 60 years

“…We added 600 ml of Buffer AL to each tube, then continued with the protocol for the QIAamp DNA Investigator kit (QIAGEN GmbH, Hilden, Germany). DNA quantification was performed using Applied Biosystems 7500 RealTime PCR System (Life Technologies/Thermo Fisher Scientific, South San Francisco, CA, USA) [1]; PCR amplification was performed using AmpFlSTR Identifiler Plus PCR Amplification Kit. Electrophoresis was performed using the Applied Biosystems 3130xl Genetic Analyzer.…”

Multi-Informative and Specific Detection of Blood in Fingermarks via MALDI-MS Based Strategies

“…Several studies have also evaluated the effect of haem-reactive tests and protein-dyes on the success and sensitivity of DNA recovery and analysis, for example demonstrating a reduction in yield by a factor between 2 and 12 [129], especially if the process requires a de-staining step. No short-term effect on DNA amplification has been observed unless considering the discontinued benzidine [108] or LMG [142], which merely requires neutralisation before DNA analysis [143]. Long-term exposure, on the other hand, has been shown to affect DNA integrity and increase degradation [108,129,144], resulting in the recommendation to perform DNA analysis within a maximum of 30 days after enhancement, at which point e.g.…”