2011
DOI: 10.1016/j.chroma.2011.04.062
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Influence of protein adsorption kinetics on breakthrough broadening in membrane affinity chromatography

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Cited by 28 publications
(23 citation statements)
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“…proportional to the reciprocal of the phase ratio. The assumption of fast adsorption in convective chromatographic media is valid for membrane and monolithic chromatography and it is reasonable to expect a similar behavior for C‐CP fibers .…”
Section: Resultsmentioning
confidence: 98%
“…proportional to the reciprocal of the phase ratio. The assumption of fast adsorption in convective chromatographic media is valid for membrane and monolithic chromatography and it is reasonable to expect a similar behavior for C‐CP fibers .…”
Section: Resultsmentioning
confidence: 98%
“…The shear rates realized in this centrifugation process (approximately 10 000–50 000/s) are far greater than are present in packed bed or monolithic columns. Herein, lies the advantages of the basic C‐CP column/tip structure; short diffusion distances to nonporous surfaces where convective diffusion (as found in monolithic materials ) likely controls mass transport . While greater surface order has been documented under conditions of high shear, it is also clear that adsorption at low linear velocities (and thus longer exposure times) would increase the tendency for proteins to denature on the hydrophobic surfaces, limiting molar surface coverage and likely perturbing capture efficiencies.…”
Section: Resultsmentioning
confidence: 99%
“…It is interesting to note that elution is partly triggered at a pH value between 4.50 and 4.75, but it is not until a pH of 4.25-3.75 that most of the adsorbed IgG is actually eluted. This observation suggests that the affinity interaction is not homogeneous, possibly due to the different IgG binding sites on the Protein A molecule [39,40], as well as differences in the orientation and immobilization of the Protein A ligands [41][42][43], but mostly due to the different variants of IgG present in the polyclonal feed solution. Indeed, as reported by Duhanel et al, the use of a pH elution gradient from Protein A resins has an effect on the separation of IgG variants and in particular, between IgG 1 and IgG 2 , with IgG 2 that elutes at higher pH and IgG 1 that elutes at lower pH values, while IgG 4 distributes equally in the whole pH range [44].…”
Section: Elution Studymentioning
confidence: 86%