Influence of Protein Size on Surface-Enhanced Raman Scattering (SERS) Spectra in Binary Protein Mixtures

Avci, Ertuğ; Çulha, Mustafa

doi:10.1366/13-07445

Cited by 8 publications

(11 citation statements)

References 50 publications

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“…The silver nanoparticles (AgNPs) were synthesized using the method reported by Leopold and Lendl and also used in our previous study [24,37]. Briefly, 10 ml of hydroxlyamine hydrochloride (1.5×10 −2 M) and sodium hydroxide (3×10 −2 M) mixture was added to 90 ml of AgNO 3 solution (1.11× 10 −3 M) and stirred for 15 min.…”

Section: Synthesis Of Agnp Colloidal Suspensionmentioning

confidence: 99%

“…The fluorescence quenching is another advantage of the technique to mention. Due to its remarkable and versatile features, this technique has been used heavily for detection and identification of bacteria [15][16][17][18][19][20][21], cells [22], tissues [23], and biomolecules [24] within the last decade. Therefore, SERS has a potential to be used for diagnostic purposes in clinical settings and several studies have been published on the way to realize this goal [25].…”

Section: Introductionmentioning

confidence: 99%

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Discrimination of urinary tract infection pathogens by means of their growth profiles using surface enhanced Raman scattering

et al. 2015

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Urinary tract infection (UTI) is a widespread infection and affects millions of people around the globe. The gold standard for identification of microorganisms causing infection is urine culture. However, current methods require at least 24 h for the results. In clinical settings, identification and discrimination of bacteria with less time-consuming and cheaper methods are highly desired. In recent years, the power of surface-enhanced Raman scattering (SERS) for fast identification of bacteria and biomolecules has been demonstrated. In this study, we show discrimination of urinary tract infection causative pathogens within 1 h of incubation using principal component analysis (PCA) of SERS spectra of seven different UTI causative bacterial species. In addition, we showed differentiation of them at their different growth phases. We also analyzed origins of bacterial SERS spectra and demonstrated the highly dynamic structure of the bacteria cell wall during their growth. Graphical Abstract Collection of bacteria from urine sample, and their discrimination using their SERS spectra and multivariate analysis.

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Section: Synthesis Of Agnp Colloidal Suspensionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Discrimination of urinary tract infection pathogens by means of their growth profiles using surface enhanced Raman scattering

et al. 2015

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“…SERS can be also used for the detection of proteins in a mixture [220,221]. An approach combining the separation of proteins using polyacrylamide gel electrophoresis (PAGE) and transferring the protein spots onto a cellulose membrane (western blotting) and detecting with SERS after staining with colloidal AgNPs was reported [208].…”

Section: Sers Of Proteinsmentioning

confidence: 99%

Fundamentals and applications of SERS-based bioanalytical sensing

et al. 2017

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Abstract:Plasmonics is an emerging field that examines the interaction between light and metallic nanostructures at the metal-dielectric interface. Surface-enhanced Raman scattering (SERS) is a powerful analytical technique that uses plasmonics to obtain detailed chemical information of molecules or molecular assemblies adsorbed or attached to nanostructured metallic surfaces. For bioanalytical applications, these surfaces are engineered to optimize for high enhancement factors and molecular specificity. In this review we focus on the fabrication of SERS substrates and their use for bioanalytical applications. We review the fundamental mechanisms of SERS and parameters governing SERS enhancement. We also discuss developments in the field of novel SERS substrates. This includes the use of different materials, sizes, shapes, and architectures to achieve high sensitivity and specificity as well as tunability or flexibility. Different fundamental approaches are discussed, such as label-free and functional assays. In addition, we highlight recent relevant advances for bioanalytical SERS applied to small molecules, proteins, DNA, and biologically relevant nanoparticles. Subsequently, we discuss the importance of data analysis and signal detection schemes to achieve smaller instruments with low cost for SERS-based point-of-care technology developments. Finally, we review the main advantages and challenges of SERS-based biosensing and provide a brief outlook.

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“…88 Binary mixtures of cytochrome c, lysozyme, Hb, HSA, apotransferrin, IgG, fibrinogen, and IgA were discriminated using principal component analysis on the observed SERS spectra. 89 The binary mixtures were based on the size of the proteins: Lys-apoTrf, Cyt c-Hb, HSA-Fb, and IgG-IgA. They reported that the affinity between the protein and NP was significantly dependent on the charge of the protein.…”

Section: Amino Acids Peptides and Proteinsmentioning

confidence: 99%

“…A larger protein may not be able to adhere to the NP through weak ionic interactions unless a protein has covalent interactions to the NP. 89 …”

Section: Amino Acids Peptides and Proteinsmentioning

confidence: 99%

Bioanalytical applications of surface-enhanced Raman spectroscopy: de novo molecular identification

Nguyen

Peters

Schultz

2017

Reviews in Analytical Chemistry

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Surface enhanced Raman scattering (SERS) has become a powerful technique for trace analysis of biomolecules. The use of SERS-tags has evolved into clinical diagnostics, the enhancement of the intrinsic signal of biomolecules on SERS active materials shows tremendous promise for the analysis of biomolecules and potential biomedical assays. The detection of the de novo signal from a wide range of biomolecules has been reported to date. In this review, we examine different classes of biomolecules for the signals observed and experimental details that enable their detection. In particular, we survey nucleic acids, amino acids, peptides, proteins, metabolites, and pathogens. The signals observed show that the interaction of the biomolecule with the enhancing nanostructure has a significant influence on the observed spectrum. Additional experiments demonstrate that internal standards can correct for intensity fluctuations and provide quantitative analysis. Experimental methods that control the interaction at the surface are providing for reproducible SERS signals. Results suggest that combining advances in methodology with the development of libraries for SERS spectra may enable the characterization of biomolecules complementary to other existing methods.

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Influence of Protein Size on Surface-Enhanced Raman Scattering (SERS) Spectra in Binary Protein Mixtures

Cited by 8 publications

References 50 publications

Discrimination of urinary tract infection pathogens by means of their growth profiles using surface enhanced Raman scattering

Discrimination of urinary tract infection pathogens by means of their growth profiles using surface enhanced Raman scattering

Fundamentals and applications of SERS-based bioanalytical sensing

Bioanalytical applications of surface-enhanced Raman spectroscopy: de novo molecular identification

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