Influence of repeat numbers on self-assembly rates of repetitive recombinant spider silk proteins

Humeník, Martin; Magdeburg, Michael; Scheibel, Thomas

doi:10.1016/j.jsb.2014.03.010

Cited by 68 publications

(127 citation statements)

References 51 publications

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“…TEM as well as AFM revealed fibrillar structures (Figure A,B), exhibiting a length of around 200–500 nm, average diameters of 15 nm (Figure A), and heights in the range of 1.0–1.3 nm (Figure B). Length and diameter of eMaSp1s fibrils is consistent with values reported earlier for recombinant spider silk fibrils …”

Section: Resultssupporting

confidence: 90%

Characterization of Hydrogels Made of a Novel Spider Silk Protein eMaSp1s and Evaluation for 3D Printing

Thamm

DeSimone

Scheibel

2017

Macromolecular Bioscience

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Recombinantly produced spider silk proteins have high potential for bioengineering and various biomedical applications because of their biocompatibility, biodegradability, and low immunogenicity. Here, the recently described small spider silk protein eMaSp1s is assembled into hydrogels, which can be 3D printed into scaffolds. Further, blending with a recombinantly produced MaSp2 derivative eADF4(C16) alters the mechanical properties of the resulting hydrogels. Different spider silk hydrogels also show a distinct recovery after a high shear stress deformation, exhibiting the tunability of their features for selected applications.

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Section: Resultssupporting

confidence: 90%

Characterization of Hydrogels Made of a Novel Spider Silk Protein eMaSp1s and Evaluation for 3D Printing

Thamm

DeSimone

Scheibel

2017

Macromolecular Bioscience

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“…19,[22][23][24]49,55,[58][59][60][61][62] Here, the protein was modified with different tags for stimulating internalization of respective protein particles by HeLa cells (Fig. 19,[22][23][24]49,55,[58][59][60][61][62] Here, the protein was modified with different tags for stimulating internalization of respective protein particles by HeLa cells (Fig.…”

Section: Spider Silk Protein Modificationmentioning

confidence: 99%

Enhanced cellular uptake of engineered spider silk particles

et al. 2015

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Drug delivery systems allow tissue/cell specific targeting of drugs in order to reduce total drug amounts administered to an organism and potential side effects upon systemic drug delivery. Most drug delivery systems are polymer-based, but the number of possible materials is limited since many commercially available polymers induce allergic or inflammatory responses or lack either biodegradability or the necessary stability in vivo. Spider silk proteins represent a new class of (bio)polymers that can be used as drug depots or drug delivery systems. The recombinant spider silk protein eADF4(C16), which can be processed into different morphologies such as particles, films, or hydrogels, has been shown to fulfil most criteria necessary for its use as biomaterial. Further, eADF4(C16) particles have been shown to be well-suited for drug delivery. Here, a new method was established for particle production to reduce particle size and size distribution. Importantly, cellular uptake of these particles was shown to be poor in HeLa cells. Therefore, variants of eADF4(C16) with inversed net charge or incorporated cell penetrating peptides and receptor interacting motifs were tested, showing much better cellular uptake. Interestingly, uptake of all silk variant particles was mainly achieved by clathrin-mediated endocytosis.

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“…The nano‐ and micro‐scale self‐assemblies are constructed mainly of nanoparticles, nanofibrils, microspheres, and vesicles, whereas macro‐scale self‐assembly focuses on developing macroscopic materials, such as hydrogels and fibers. Most of these self‐assembly processes were conducted in aqueous solutions . However, some RSSPs, such as spidroin variants from the C‐terminal domain of the major ampullate and cylindriform silk, as well as major ampullate spidroin‐like RSSP with a high ratio of hydrophobic polyalanine regions, have limited water‐solubility.…”

Section: Multiscale Self‐assembly Of the Rsspsmentioning

confidence: 99%

De Novo Design of Recombinant Spider Silk Proteins for Material Applications

Zheng

Ling

2018

Biotechnology Journal

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Spider silks are well known for their superior mechanical properties that are stronger and tougher than steel despite being assembled at close to ambient conditions and using water as the solvent. However, it is a significant challenge to utilize spider silks for practical applications due to their limited sources. Fortunately, genetic engineering techniques offer a promising approach to produce useable amounts of spider silk variants. Starting from these recombinant spider silk proteins, a series of experiments and simulations strategies are developed to improve the recombinant spider silk proteins (RSSP) material design and fabrication with the aim of biomimicking the structure-property-function relationships of spider silks. Accordingly, in this review, the authors first introduce the structure-property-function relationship of spider silks. Then, the recent progress in the genetic synthesis of RSSPs is discussed and their related multiscale self-assembly behaviors is summarized. Finally, the authors outline works utilizing multiscale modeling to assist RSSP material design.

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Influence of repeat numbers on self-assembly rates of repetitive recombinant spider silk proteins

Cited by 68 publications

References 51 publications

Characterization of Hydrogels Made of a Novel Spider Silk Protein eMaSp1s and Evaluation for 3D Printing

Characterization of Hydrogels Made of a Novel Spider Silk Protein eMaSp1s and Evaluation for 3D Printing

Enhanced cellular uptake of engineered spider silk particles

De Novo Design of Recombinant Spider Silk Proteins for Material Applications

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