Influence of rice development on the function of bacterial blight resistance genes

Webb, Kimberly M.; Garcia, Epifania; Cruz, C. M. Vera; Leach, Jan E.

doi:10.1007/s10658-010-9668-z

Cited by 13 publications

(10 citation statements)

References 31 publications

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“…Dominant but un-cloned R gene Xa7 was first identified in DV85 rice cultivar and was reported to be predominate in Aus cultivars of Bangladesh [18,75,76]. Though it is not cloned yet, its durable resistance and effectiveness in high temperature makes it a desirable target gene in modern breeding programs especially in tropical country like Bangladesh [49,77]. In the present study, we identified maximum 83.78% germplasm (62 out of 74) which carries Xa7 gene.…”

Section: Discussionmentioning

confidence: 99%

“…The STS markers utilized in the study were found highly efficient to identify the resistant genes. Dominant resistant gene Xa4, first reported by [48], functioned via cell wall-associated kinase synthesis and confers a race-specific but plant growth stage nonspecific resistance towards Xoo strains [49][50][51]. Moreover, [51] deciphered the role of Xa4 gene in improving certain quantitative traits such as, plant height.…”

Section: Discussionmentioning

confidence: 99%

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Exploring of bacterial blight resistance in landraces, mining of resistant gene(s) and genetic divergence of resistant germplasm through molecular markers and quantitative traits

Anik¹,

Nihad²,

Hasan³

et al. 2021

Preprint

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Bacterial blight, one of the oldest and severe diseases of rice poses a major threat towards global rice production and food security. Thereafter, sustainable management of this disease has given paramount importance globally. In the current study, we explored 792 landraces to evaluate their disease reaction status against three highly virulent strains ( BXo69 , BXo87 and BXo93 ) of Xanthomonas oryzae pv . oryzae ( Xoo ). Subsequently, we intended to identify the possible candidate resistant ( R ) genes responsible for the resistant reaction using six STS markers correspond to Xa4 , xa5 , Xa7 , xa13 , Xa21 and Xa23 genes and finally, we evaluated morphological and molecular diversity of the potential bacterial blight resistant germplasm using quantitative traits and ISSR markers. Based on pathogenicity test, a single germplasm was found as highly resistant while, 33 germplasm were resistant and 40 were moderately resistant. Further molecular study on these 74 germplasm divulged that 41 germplasm carried Xa4 gene, 15 carried xa5 gene, 62 carried Xa7 gene, 33 carried xa13 gene, and 19 carried Xa23 gene. Only a single germplasm consisted of Xa21 gene. Interestingly, we found a wide range of gene combinations ranged from 2 to 4 genes among the germplasm resistant to bacterial blight and G3 genotype (Acc. No. 4216; highly resistant) having Xa4 , Xa7 , xa13 , Xa21 and G43 genotype (Acc. No. 1523; resistant) having Xa4 , xa5 , xa13 and Xa23 gene combination being the most effective against all the Xoo strains. Nonetheless, UPGMA dendrogram and heatmap analysis based on quantitative traits identified two important clusters viz. cluster-III and cluster-VIII with multiple desired traits However, genetic similarity based on ISSR marker data pointed out 3 germplasm, namely, G20 (Acc. No. 4004; contained Xa4, Xa7, xa13 genes), G17 (Acc. No. 3981; contained Xa7, xa13 genes) and G6 (Acc. No. 991; contained Xa4, Xa7, xa13, Xa23 genes) having comparatively lower genetic similarity (0.30, 0.37 and 0.38, respectively) with elite variety BRRI dhan28. Notably, Mantle test of molecular and morphological data indicated that there was a positive correlation (r = 0.113) between them. The outcome of this study would enrich and diversify the rice gene pool and would be useful for the development of durable bacterial blight resistant varieties.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Exploring of bacterial blight resistance in landraces, mining of resistant gene(s) and genetic divergence of resistant germplasm through molecular markers and quantitative traits

Anik¹,

Nihad²,

Hasan³

et al. 2021

Preprint

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“…Xa7 gene is well known for conferring durable resistance against bacterial leaf blight, which has been verified by a 10-year cropping period (Webb et al 2010). Furthermore, increased effectiveness has been reported for Xa7-mediated resistance under high temperature and drought stresses (Dossa et al 2020).…”

Section: Introductionmentioning

confidence: 90%

“…oryzae (Xoo) have become important models for understanding the fundamental aspects of plant disease resistance and bacterial pathogenesis, as well as other aspects of plant and microbial biology, with implications for innate animal immunity too (Niño-Liu et al 2006). Xa4 gene mediates a race-specific and durable resistance to Xoo at all stages of rice growth in many countries (Webb et al 2010). Xa4 gene was first incorporated into commercial rice varieties in the early 1979s and has been one of the most widely exploited resistance genes in breeding programs in at least 54 Asian, African, South American, and Mesoamerican countries (Khush and Virk 2005).…”

Section: Introductionmentioning

confidence: 99%

Phenotype and genotype variability of interspecific rice lines related to bacterial leaf blight resistance (Xanthomonas oryzae pv. oryzae) character

et al. 2021

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Abstract. Yuriyah S, Darnaedi D, Setia TM, Windarsih G, Utami DW. 2021. Phenotype and genotype variability of interspecific rice lines related to bacterial leaf blight resistance (Xanthomonas oryzae pv. oryzae) character. Biodiversitas 22: 4123-4130. Wild rice species are the source of the gene pool for rice genetic diversity. The cross-species crosses (interspecific crosses) play an important role in breeding, namely in terms of expanding the diversity of desirable characters, such as disease resistance and improvement in yield potential. Currently, the genes from wild rice species have been successfully introgressed into cultivated rice so that they can overcome the rice production constraints, including the introgression of genes for the bacterial leaf blight (BLB) resistance. The purpose of this study was to analyze the variability of phenotype and genotype performance of lines derived from wild rice species for the character of resistance to BLB disease. A total of 33 selected backcross lines derived from wild rice species of O. rufipogon and O. glaberrima as the donor parents and 2 control varieties (resistant and susceptible to BLB disease), were used in this study. The evaluation of phenotype and genotype of resistance characters to BLB disease was carried out during a vegetative phase of the plant. The resistance evaluation was carried out in a greenhouse, while the genotype performance was analyzed using 4 STS markers linked to Xa4, Xa7, and Xa13 genes. The result of the phenotype evaluation showed that there were variations among the resistance of the tested lines. Three lines derived from a Situ Bagendit/Oryza rufipogon cross were resistant to all BLB races used in the testing. Those three lines indicated to have the allele of the Xa7 resistance gene based on the genotype performance which grouped with the Code variety that had the Xa7 resistance gene.

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“…Galur isogenik bermanfaat dalam monitoring keparahan serangan dan distribusi ras Xoo (Ogawa et al, 1988). Di samping itu, galur-galur isogenik juga dapat digunakan sebagai standar diferensial untuk mengetahui perbedaan respons patogenisitas atau virulensi suatu ras (Hayashi dan Fukuta, 2007;Khoshkdaman et al, 2012;Loan et al, 2006;Noh et al, 2016;Tsunematsu et al, 2000) dan untuk evaluasi ketahanan galur hasil persilangan (silang balik) dalam rangka perakitan varietas tahan HDB (Webb et al, 2010).…”

Section: Pendahuluanunclassified

Identifikasi Gen Ketahanan terhadap (Xanthomonasoryzae pv. Oryzae), penyebab Penyakit Hawar Daun Bakteri padaPlasma Nutfah Padi Lokal Sulawesi Selatan

Yuriyah¹,

Nurani

Utami³

et al. 2018

J. AgroBiogen

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One of the limiting factors on rice production, especially in South Sulawesi, is the bacterial leaf blight (BLB) disease infection caused by Xanthomonas oryzae pv. oryzae (Xoo). The use of resistant variety is considered as the most effective method to control Xoo. Local cultivar could serve as resistance source for Xoo resistance. The objectives of this research were to evaluate and identify markers for Xoo resistance in rice local cultivars from South Sulawesi based on comparison with the resistance response of the differential varieties and association analysis between resistance phenotype and genotype of molecular markers. Three Xoo races (race III, IV, and VIII) were tested on IRBB differential lines and local cultivars. The genotype analysis was done by using the molecular marker linked to Xoo resistance. Meanwhile, the association analysis was done by combination analysis (U-joint) using generalized linear model (GLM). The resistance test result showed that single gene isogenic lines (IRBB 5, IRBB 7, and IRBB 21), multiple genes isogenic lines (IRBB 50, IRBB 52, IRBB 53, IRBB 54, IRBB 56, IRBB 58, IRBB 64, and IRBB 66), and Ase Andele accession were resistant to the three Xoo races. Association test obtained one significant marker that associated with the resistance to race III (marker Xa26-STS2), four markers significantly associated with the resistance to race IV (Xa1-STS15, Xa4-STS44, xa13-STS51, and Xa21-STS6), and two markers significantly associated with the resistance to race VIII (Xa7-STS57 and RM 20589). Keywords:Resistance gene, bacterial leaf blight, germplasm. ABSTRAKSalah satu faktor pembatas produksi padi, di Sulawesi Selatan khususnya, adalah serangan penyakit hawar daun bakteri (HDB) yang disebabkan oleh Xanthomonas oryzae pv. oryzae (Xoo). Upaya yang dipandang efektif untuk mengendalikan penyakit HDB adalah penanaman varietas tahan. Perakitan varietas padi dengan menggunakan gen-gen tahan dari berbagai kultivar berpeluang menghasilkan varietas tahan HDB. Kultivar lokal berperan sebagai salah satu sumber keragaman genetik untuk beberapa sifat ketahanan penyakit. Penelitian ini bertujuan mengevaluasi dan mengidentifikasi marka penanda gen ketahanan penyakit hawar daun bakteri pada padi lokal Sulawesi Selatan berdasarkan sistem standar diferensial dan analisis molekuler. Analisis uji ketahanan fenotipe dilakukan menggunakan tiga ras HDB (ras III, IV, dan VIII) dan galur diferensial (galur IRBB). Analisis genotipe dilakukan menggunakan marka molekuler terkait ketahanan terhadap HDB yang ditampilkan sebagai dendrogram keragaman. Analisis asosiasi dilakukan dengan analisis gabungan (U-joint) menggunakan generalized linear model (GLM). Hasil pengujian ketahanan menunjukkan galur isogenik dengan gen tunggal (IRBB 5, IRBB 7, dan IRBB 21), galur isogenik dengan multipel gen (IRBB 50, IRBB 52, IRBB 53, IRBB 54, IRBB 56, IRBB 58, IRBB 64, dan IRBB 66), dan aksesi Ase Andele bersifat tahan terhadap ketiga ras uji. Dari uji asosiasi, diperoleh satu marka signifikan yang berasosiasi dengan k...

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Influence of rice development on the function of bacterial blight resistance genes

Abstract: Disease resistance genes most commonly used in breeding programs are single, dominant genes with relative effectiveness that is sometimes influ-

Cited by 13 publications

References 31 publications

Exploring of bacterial blight resistance in landraces, mining of resistant gene(s) and genetic divergence of resistant germplasm through molecular markers and quantitative traits

Exploring of bacterial blight resistance in landraces, mining of resistant gene(s) and genetic divergence of resistant germplasm through molecular markers and quantitative traits

Phenotype and genotype variability of interspecific rice lines related to bacterial leaf blight resistance (Xanthomonas oryzae pv. oryzae) character

Identifikasi Gen Ketahanan terhadap (Xanthomonasoryzae pv. Oryzae), penyebab Penyakit Hawar Daun Bakteri padaPlasma Nutfah Padi Lokal Sulawesi Selatan

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