The low diversification of rootstock genotypes in orchards limits the expansion of the citrus industry, restricting increases in productivity and cost-saving via phytosanitary treatments and other horticultural practices. Therefore, the aim of this study was to assess the impact of rootstock genotypes on tree development and industrial properties of ‘Valencia’ sweet orange juice (Citrus sinensis). Twenty rootstock genotypes were evaluated by measuring tree growth and industrial properties of orange juices, including ‘Trifoliata’ hybrids with tangerine (citrandarins) and grapefruit (citrumelos), as well ‘Rangpur’ lime and other potential rootstocks. The experimental orchard was planted in Rancho Alegre, PR, Brazil, under clay soil and subtropical rainfed conditions. A randomized block design with four replicates was used. Trees grown on IPEACS–239 and IPEACS–256 citrandarins, and on ‘US–802’ pummelo hybrid had low vigor, high production efficiency and high industrial properties of orange juice, and are therefore potential alternatives for high-density plantings. The F.80–3 and F.80–5 citrumelos also had good dwarfing potential and high production efficiency, but lower industrial properties of juice compared to the other ‘Trifoliata’ hybrid rootstocks. Trees grown on ‘US–812’ citrandarin rootstock had low vigor, good productive performance, accumulated production and production efficiency similar to ‘Rangpur’ lime, and high industrial properties of juices. Although the ‘Rangpur’ lime and the ‘Florida’ rough lemon allowed high yields, the trees are very vigorous, with low-quality fruits. A Quick Reference Chart was created to provide practical and objective identification of the best rootstock alternatives for ‘Valencia’ orange trees in terms of tree development and industrial properties of juices.