Influence of selenium on growth, lipid peroxidation and antioxidative enzyme activity in melon (Cucumis melo L.) seedlings under salt stress

Abstract

The objective of this study was to investigate the effect of exogenous selenium (Se) supply (0, 2, 4, 8, 16 μM) on the growth, lipid peroxidation and antioxidative enzyme activity of 100 mM NaCl-stressed melon (<em>Cucumis melo</em> L.) seedlings. Salt stress significantly reduced the growth attributes including stem length, stem diameter, dry weight and increased antioxidative enzyme activity [superoxide dismutase (SOD), peroxidase (POD), catalase (CAT)]. Moreover, the plant exhibited a significant increase in electrolyte leakage and malondialdehyde (MDA) content under NaCl stress. Se supplementation not only improved the growth parameters but also successfully ameliorated the adverse effect caused by salt stress in melon seedlings. However, the mitigation of NaCl-stressed seedlings was different depending on the Se concentration. At lower concentrations (2–8 μM), Se improved growth and acted as antioxidant by inhibiting lipid peroxidation and increasing in SOD and POD enzymes activity under salt stress. At higher concentrations (16 μM), Se exerted diminished beneficial effects on growth. Whereas CAT activity was enhanced. The result indicated that Se supplementation had a positive physiological effect on the growth and development of salt-stressed melon seedlings.

Selenite is More Efficient than Selenate in Alleviation of Salt Stress in Lettuce Plants

“…One of the causes of lipid peroxidation is accumulation of H 2 O 2 which, like other ROS, attacks lipid membranes (Hawrylak-Nowak, 2013;KeLing et al, 2013). These results, indicating antioxidant activity of Se in the salt-stressed plants, are in agreement with those obtained by Kong et al (2005), Hawrylak-Nowak (2009), Walaa et al (2010), Hasanuzzaman et al (2011), KeLing et al (2013 and Diao et al (2014).…”

“…Over-accumulation of these ions generates many physiological disorders in plants, but Cl -ions are considered to be more toxic than Na + (Hasanuzzaman et al, 2013). The results of previous studies indicated that exogenously applied Se both in the form of selenate (Kong et al, 2005;Hawrylak-Nowak, 2009;Hasanuzzaman et al, 2011) and selenite (Walaa et al, 2010;KeLing et al, 2013;Diao et al, 2014) can be beneficial for plants grown under salt stress. Nevertheless, in the above studies the effectiveness of selenate and selenite in alleviation of salinity stress was not compared.…”

“…One of the causes of lipid peroxidation is accumulation of H 2 O 2 which, like other ROS, attacks lipid membranes (Hawrylak-Nowak, 2013;KeLing et al, 2013). These results, indicating antioxidant activity of Se in the salt-stressed plants, are in agreement with those obtained by Kong et al (2005), Hawrylak-Nowak (2009), Walaa et al (2010), Hasanuzzaman et al (2011), KeLing et al (2013 and Diao et al (2014). However, in this study the highest growth-stimulating effect of exogenous Se noted at 2 μM Se IV was associated with simultaneous reduction of lipid peroxidation and H 2 O 2 accumulation, mainly in the root tissues.…”

“…Lettuce was demonstrated to be a moderately salt sensitive vegetable crop (Shannon and Grieve, 1999). Although some reports have shown that Se presents a promising potential for use in the conditions of salt stress (Hawrylak-Nowak, 2009;Walaa et al, 2010;Hasanuzzaman et al, 2011;KeLing et al, 2013), the influence of the applied chemical form of Se on its activity in salt-stressed plants had not been studied earlier. Therefore, the aim of the present study was to compare the effectiveness of two inorganic chemical forms of Se (selenate and selenite) in mitigation of NaCl-induced salt stress in lettuce plants.…”

Involvement of Selenium in Protective Mechanisms of Plants under Environmental Stress Conditions – Review

“…In Se-supplemented plants growing under waterdeficit conditions the higher (in comparison to plants cultured without Se addition) activities of such antioxidative enzymes as CAT and POX were detected (Xiaoqin et al, 2009;Habibi, 2013;Proietti et al, 2013). However, Ibrahim (2014) and KeLing et al (2013) registered also an increase in SOD activity under these conditions. Moreover, the content of non-enzymatic antioxidants (AsA and GSH) (Ibrahim, 2014).…”