Influence of siderophores and iron on Mycobacterium bovis isolation from pathological material

Abstract

The article presents the results of studying the effect of siderophores and iron on the isolation of Mycobacterium bovis from pathological material. It has been established that the simultaneous presence of iron and siderophore from M. phlei in the nutrient medium makes it possible to detect the growth of M. bovis from pathological material 6–8 days earlier; ensures the growth of more colonies and bacterial mass. The presence of heterologous to mycobacteria siderophore (from Nocardia spp.) in the medium reduces the elective (growth) properties of the medium. Siderophores found in the culture filtrate or alcoholic extract of M. phlei can be valuable additives to culture media for the accelerated isolation of M. bovis from pathological material

Mycobacterium avium Subspecies paratuberculosis in Asymptomatic Zoo Herbivores in Poland

“…RT-PCR also achieved positive results in the case of seven other species ( Table 3 ). All seven species have previously been confirmed to harbour MAP: pudu [ 28 ], guanaco [ 29 , 30 ], European bison [ 7 ], giraffe [ 8 ], Bactrian camel [ 31 ], alpaca [ 29 , 32 ], and domestic goat [ 33 ].…”