Influence of spermine on intestinal maturation of the glycoprotein glycosylation process in neonatal rats

Greco, Sandrine; Hugueny, Irène; George, Pascal; Perrin, Pascale; Louisot, Pierre; Biol, Marie-Claire

doi:10.1042/bj3450069

Cited by 10 publications

(7 citation statements)

References 43 publications

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“…The most profound changes are observed in the epithelium of the small intestine. Developmental adaption of small intestinal enterocytes is considered to be preprogrammed, but several studies have suggested that external stimuli, such as microbial colonization and diet, may modulate the timing of age-related changes during early postnatal life (1)(2)(3)(4)(5). The joint action of environmental and intrinsic stimuli may therefore be responsible for initiating the adaptive changes and influence the precise timing and the level of expression of specific intestinal markers.…”

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confidence: 99%

Time-resolved Quantitative Proteome Analysis of In Vivo Intestinal Development

Hansson

Panchaud

Favre

et al. 2011

Molecular & Cellular Proteomics

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Postnatal intestinal development is a very dynamic process characterized by substantial morphological changes that coincide with functional adaption to the nutritional change from a diet rich in fat (milk) to a diet rich in carbohydrates on from weaning. Time-resolved studies of intestinal development have so far been limited to investigation at the transcription level or to single or few proteins at a time. In the present study, we elucidate proteomic changes of primary intestinal epithelial cells from jejunum during early suckling (1-7 days of age), middle suckling (7-14 days), and weaning period (14 -35 days) in mice, using a label-free proteomics approach. We show differential expression of 520 proteins during intestinal development and a pronounced change of the proteome during the middle suckling period and weaning. Proteins involved in several metabolic processes were found differentially expressed along the development. The temporal expression profiles of enzymes of the glycolysis were found to correlate with the increase in carbohydrate uptake at weaning, whereas the abundance changes of proteins involved in fatty acid metabolism as well as lactose metabolism indicated a nondiet driven preparation for the nutritional change at weaning. Further, we report the developmental abundance changes of proteins playing a vital role in the neonatal acquisition of passive immunity. In addition, different isoforms of several proteins were quantified, which may contribute to a better understanding of the roles of the specific isoforms in the small intestine. In summary, we provide a first, time-resolved proteome

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mentioning

confidence: 99%

Time-resolved Quantitative Proteome Analysis of In Vivo Intestinal Development

Hansson

Panchaud

Favre

et al. 2011

Molecular & Cellular Proteomics

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“…The dose and concentration of SPM selected for the present experiments approximated those used by previous investigators to induce the maturation of the intestine in animals. [10][11][12][13][14][15] The dose of SPM used in the present study is well within normal limits.…”

Section: Discussionmentioning

confidence: 70%

“…8,9) Oral administration of SPM induces precocious maturation of the intestine. [10][11][12][13][14][15] Oral polyamines have also been shown to have beneficial effects on healing 16,17) and prevention [18][19][20] of gastrointestinal damage.The gastrointestinal tract acts as a barrier against the invasion of exogenous proteins into the body. This barrier function is due to enzymes that degrade the proteins and also due to impermeability of the proteins to the membrane due to their large molecular weight.…”

mentioning

confidence: 99%

Enhancement of Gastrointestinal Absorption of Ovalbumin Caused by Spermine Induces an Increase in Plasma Histamine Levels in Mice Sensitized to Ovalbumin

Sugita

Takao

Sugino

et al. 2011

Biological & Pharmaceutical Bulletin

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The polyamines spermidine and spermine (SPM) and their precursor putrescine are ubiquitous polycationic compounds found in all mammalian cells. [1][2][3][4][5] These compounds are essential for the maintenance of cell growth in many tissues, and intracellular levels of polyamines are maintained within narrow limits.The polyamines are present in variable amounts in almost all food or foodstuffs. In humans, a typical diet might contribute hundreds of micromoles of polyamines per day to the gut lumen, 6,7) and ingested food may be the major source of polyamines in the lumen of the upper small bowel. Exogenous polyamines play a role in the maintenance of normal growth and function of the intestinal tract. 8,9) Oral administration of SPM induces precocious maturation of the intestine. [10][11][12][13][14][15] Oral polyamines have also been shown to have beneficial effects on healing 16,17) and prevention [18][19][20] of gastrointestinal damage.The gastrointestinal tract acts as a barrier against the invasion of exogenous proteins into the body. This barrier function is due to enzymes that degrade the proteins and also due to impermeability of the proteins to the membrane due to their large molecular weight. Because most food-originated proteins are digested in the gastrointestinal tract before absorption, naturally occurring protein uptake from the intestinal epithelium should be low. In reality, however, the barrier function of the small intestinal epithelium in mammals is incomplete and, to a limited extent, macromolecules can pass from the lumen into the circulation. 21,22) In an in situ loop study and in an in vivo oral absorption study, we showed that polyamines, especially SPM, can enhance the intestinal absorption of fluorescein isothiocyanatelabeled dextran (molecular weight (MW) 4400, FD-4), a hydrophilic model macromolecule, without causing any significant damage in the intestinal tract in rats.23) We also suggested that the absorption-enhancing mechanism of SPM partly includes opening the tight junctions of the epithelium via the paracellular route by measuring FD-4 permeation and transepithelial electrical resistance in the human intestinal Caco-2 cell line. We have speculated that excess absorption of macromolecules by the action of SPM, one of the compounds in food, has widespread health effects. Thus, modulation of the gastrointestinal epithelial barrier by ingested luminal polyamines may influence the induction of food allergies. Gastrointestinal permeability enhanced by daily ingestion of polyamines plays a part in the regulation of the induction of tolerance because oral tolerance induction is related to the gradual and continuous absorption of a food allergen. 24,25) In contrast, after sensitization to an antigen, an increase in gastrointestinal permeability by polyamines may facilitate the absorption of the allergen into the circulation and lead to an anaphylactic response as an immediate hypersensitivity reaction to the antigen.The aim of this study was to determine whether an SPMinduced increase...

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“…The polyamine determinations were performed as previously described (33). Briefly, the small intestines (from the gastroduodenal junction to the ileocecal junction) were quickly removed and washed with 9 g/L saline solution at 4°C.…”

Section: Methodsmentioning

confidence: 99%

“…An internal standard was added, and the proteins were discarded after precipitation. After derivatization with 1 mM 9-fluorenylmethyl chloroformate for 45 s at room temperature, the polyamines were separated by HPLC, as previously described (33), identified by their retention times, and quantified by comparison with standard concentration curves, using 1,6-hexanediamine as an internal standard.…”

Section: Methodsmentioning

confidence: 99%

Polyamine Participation in the Maturation of Glycoprotein Fucosylation, but not Sialylation, in Rat Small Intestine

Biol-N’garagba

2002

Pediatric Research

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The aim of this study was to determine the role of polyamines in the diet-related maturation of the intestinal glycoprotein glycosylation during postnatal development in the rat. The activity of ␣-2,6-sialyltransferase and the sialylated forms of glycoproteins in the intestinal brush-border membranes were found to decrease considerably after weaning, in parallel with the intestinal level of putrescine. By contrast, the activity of ␣-1,2-fucosyltransferases, the mRNA levels for two ␣-1,2-fucosyltransferase genes, FTA and FTB, and the fucosylated forms of glycoproteins all increased after weaning, in parallel with the levels of spermidine and spermine. These results suggest a possible role of polyamines in the evolution of glycosylation. The treatment of suckling rats with spermidine or spermine reproduced the high intestinal levels of these polyamines corresponding to those normally found after weaning. After these treatments, a rise in the activity of the ␣-1,2-fucosyltransferase was observed, associated with a fall in ␣-L-fucosidase activity. The ␣-1,2-fucosyltransferase FTB gene was found to be regulated at the transcriptional level, but not by its inhibitor, fuctinin. The result of these variations was the precocious appearance of several ␣-1,2-fucoproteins, which are normally found in brushborder membranes after weaning. The treatment of suckling rats with putrescine, which induced only a transitory rise in intestinal putrescine, had a similar but weaker effect on the fucosylation process than spermidine or spermine, and treatment with ornithine was ineffective. ␣-2,6-Sialylation was not affected by any of the treatments. Spermidine and spermine turned out to be more effective than putrescine for intestinal glycoprotein fucosylation, but did not affect their sialylation. Spermidine and spermine, whose intestinal levels where found to increase at weaning time, may have been partly responsible for the natural evolution of the intestinal glycoprotein fucosylation that occurred during this period. The rat small intestine is immature until the end of the third week of life, when the weaning period begins. Intestinal maturation is associated with morphologic changes, an increase in mucus production, immunologic adaptation to new microbial and nutritional antigenic contents (1), and digestive adaptation to new nutriments (2).Two types of glycoproteins play important functional roles in the small intestine. Mucins (secreted by the goblet cells) are different in composition between neonatal and mature rats, especially as regards their glycan chains (3). In the neonate, these changes may affect the permeability (4) and the barrier function of the intestine, and consequently may favor infections (5) and food allergies (6). At the apical enterocyte level, in the brush-border membranes, most of the digestive enzymes (lactase, sucrase, maltase, aminopeptidase, alkaline phosphatase) are glycoproteins whose activity changes a great deal at weaning time to enable the animal to cope with the adult solid diet (2). A shift from...

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Influence of spermine on intestinal maturation of the glycoprotein glycosylation process in neonatal rats

Cited by 10 publications

References 43 publications

Time-resolved Quantitative Proteome Analysis of In Vivo Intestinal Development

Time-resolved Quantitative Proteome Analysis of In Vivo Intestinal Development

Enhancement of Gastrointestinal Absorption of Ovalbumin Caused by Spermine Induces an Increase in Plasma Histamine Levels in Mice Sensitized to Ovalbumin

Polyamine Participation in the Maturation of Glycoprotein Fucosylation, but not Sialylation, in Rat Small Intestine

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