Influence of storage conditions on the release of growth factors in platelet-rich blood derivatives

Düregger, Katharina; Peng, Anqi; Eblenkamp, Markus

doi:10.1515/cdbme-2016-0069

Cited by 3 publications

(4 citation statements)

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“…2,10 Among many growth factors, PDGF-BB is one of the most important in the regeneration and healing of wounds or damaged tissue. 9,10 PDGF-BB levels in the three groups showed no significant difference. The interesting fact from this study is that the mean of platelet counts found in such platelet rich plasma products was not directly proportional to the levels of PDGF-BB after platelet activation.…”

Section: Discussionmentioning

confidence: 78%

“…8 The benefit of PRP lies in the number of platelets it contains, but more than that can be seen from the level of growth factors released when activated. 9 These growth factors have the highest concentration in platelets and play a very important role in the process of wound healing. 2,10 Among many growth factors, PDGF-BB is one of the most important in the regeneration and healing of wounds or damaged tissue.…”

Section: Discussionmentioning

confidence: 99%

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Analysis of Platelet Counts and Platelet-Derived Growth Factor-Bb Levels in Platelet Rich Plasma Produced with Edta as Anticoagulant in Three Different Centrifugation Methods

2020

IJPHRD

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Background: Platelet rich plasma (PRP) is an autologous product made of whole blood through centrifugation process producing high platelet concentrate in a small volume of plasma. This high platelet concentrate can lead to high growth factor levels that play important roles in the nature of thrombosis, hemostasis and wound healing. PRP has been widely used in clinical setting, however standardized procedure of PRP production has been lacking, mainly the procedure related to duration and speed of centrifugation, and also anticoagulant used. Aim:To analyze the difference between platelet counts and PDGF-BB levels in PRP produced from various preparation methods (centrifugation speed and duration), using Ethylenediaminetetraacetic Acid /EDTA as anticoagulant.Method: This study used experimental laboratory design, involving 34 healthy volunteers that met inclusion criteria. These subjects were divided into three groups with different centrifugation methods that adopted from previous study. Results:The platelet counts was found in group 1, 2, and 3 with an increase of, 2,69, 3,69, and 2,53 times from the initial platelet counts respectively and there was significant difference between platelet counts before and after treatment on three groups that used EDTA (p=0,000, p=0,003, and p=0,002 for group 1, 2 and 3 respectively). PDGF-BB levels in this group was also higher than the remaining groups. Conclusion:The highest platelet counts and PDGF-BB levels was found in PRP with EDTA produced from first spin at 2800 RPM in five minutes, and second spin at 3800 RPM in seven minutes. This study suggests further research on qualitative assessments of platelets and PDGF-BB resulted from various preparation methods (centrifugation and anticoagulant usage).

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Section: Discussionmentioning

confidence: 78%

Section: Discussionmentioning

confidence: 99%

Analysis of Platelet Counts and Platelet-Derived Growth Factor-Bb Levels in Platelet Rich Plasma Produced with Edta as Anticoagulant in Three Different Centrifugation Methods

2020

IJPHRD

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“…Generation of PRP was carried out with a fully automated centrifugation system from 11 ml of whole blood drawn from healthy donors as previously described. 31…”

Section: Methodsmentioning

confidence: 99%

“…Generation of PRP was carried out with a fully automated centrifugation system from 11 ml of whole blood drawn from healthy donors as previously described. 31 Adhesion of thrombocytes. To determine the integrity of the platelets and the ability to release growth factors, the adhesion of thrombocytes onto the printed membrane was tested.…”

Section: Application-oriented Validationmentioning

confidence: 99%

Additive-manufactured microporous polymer membranes for biomedical in vitro applications

et al. 2018

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Microscale porous membranes are used in a wide range of technical and medical applications such as water treatment, dialysis and in vitro test systems. A promising approach to control membrane properties and overcome limitations of conventional fabrication techniques is given by additive manufacturing (AM). In this study, we designed and printed a microporous membrane via digital light processing and validated its use for biomedical in vitro applications based on the example of a cell culture insert. A multi-layer technique was developed, resulting in an eight-layer membrane with an average pore diameter of 25 µm. Image analyses proved the printing accuracy to be high with small deviations for an increasing number of layers. Permeability tests with brilliant blue FCF (E133, triarylmethane dye) and growth factors comparing the printed to track-etched membranes showed similar transfer dynamics and confirmed sufficient separation properties. Overall, the results showed that printing microporous polymer membranes is possible and highlight the potential of AM for biomedical in vitro applications such as cell culture inserts, scaffolds for tissue engineering or bioreactors.

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Revitalizing female fertility: platelet-rich plasma – hype or hope?

Serdarogullari,

Raad,

Makieva

et al. 2024

Reproductive BioMedicine Online

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Influence of storage conditions on the release of growth factors in platelet-rich blood derivatives

Cited by 3 publications

References 4 publications

Analysis of Platelet Counts and Platelet-Derived Growth Factor-Bb Levels in Platelet Rich Plasma Produced with Edta as Anticoagulant in Three Different Centrifugation Methods

Analysis of Platelet Counts and Platelet-Derived Growth Factor-Bb Levels in Platelet Rich Plasma Produced with Edta as Anticoagulant in Three Different Centrifugation Methods

Additive-manufactured microporous polymer membranes for biomedical in vitro applications

Revitalizing female fertility: platelet-rich plasma – hype or hope?

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