Influence of storage conditions on normal plasma amino-acid concentrations

Eijk, Hans M.H. van; Dejong, C.H.C.; Deutz, N.E.P.; Soeters, Peter B.

doi:10.1016/0261-5614(94)90028-0

Cited by 58 publications

(32 citation statements)

References 21 publications

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“…Negligible changes in the 1 H NMR spectra of plasma samples stored for up to 6 days at +4°C (Otvos et al 1991) or up to 9 months at -80°C (Deprez et al 2002) have been reported, but a decrease in triglyceride resonances after storage or with an increased number of freeze-thaw cycles has also been described (Bell et al 1987;Bell et al 1988). Brinc et al (2012) detected no significant timedependent concentration changes for the majority of biochemical markers tested over up to 13 months, while other authors reported changes in amino acid concentrations after up to 6 months (Schaefer et al 1987;van Eijk et al 1994). …”

Section: Introductionmentioning

confidence: 86%

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NMR-based metabonomics of bovine blood: an investigation into the effects of long term storage on plasma samples

2013

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Section: Introductionmentioning

confidence: 86%

“…Brinc et al 2012;Deprez et al 2002;van Eijk et al 1994) Research into the effects of long term storage of plasma or serum samples is sparse (e.g. Hustad et al 2012;Gislefoss et al 2008) or, in the context of metabolomics, non-existent.…”

Section: Discussionmentioning

confidence: 99%

NMR-based metabonomics of bovine blood: an investigation into the effects of long term storage on plasma samples

2013

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“…The levels of arginine, citrulline and ornithine concentrations in plasma were determined using a fully automated HPLC system as described previously (Van Eijk et al, 1994).…”

Section: Measurement Amino Acids In Plasmamentioning

confidence: 99%

Effects of intravenous arginine on the healing of human skin graft donor sites: A randomized controlled trial

Lin

et al. 2015

Bangladesh J Pharmacol

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We expounded the effect of intravenous arginine on the healing of human skin graft donor sites. This randomized study was double blind and controlled by placebos from October 2012 to May 2015. Nine patients were selected into the arginine group and 10 were selected into the control group. There was no significance in age, weight, BMI, CRP, albumin and total plasma protein in arginine and control groups. Angiogenesis, re-epithelialization and neutrophil influx of wound healing were measured. The concentrations of plasma amino acid were measured to evaluate our intervention. In our study, plasma arginine and ornithine concentrations in arginine group were the highest after arginine-treated at 2 days. Supplementation of intravenous arginine could not affect on healing of human skin graft donor sites, improve angiogenesis, reepithelialization or neutrophil influx in rats. Article Info

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“…The circulating concentration of GLN (0.6-0.9 mmol/l) is maintained at a constant level (9) and depends on the rate of GLN uptake and release by the various organs in the body (10). Following uptake, GLN is converted to glutamate and ammonia by glutaminase in the mitochondria.…”

Section: Introductionmentioning

confidence: 99%

Expression of PPARγ is reduced by medium supplementation with L-glutamine in human colorectal Caco-2 cells

Fiatte¹,

Huin²,

Collet³

et al. 1998

Int J Mol Med

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Abstract. Peroxisome proliferator-activated receptor-γ (PPARγ) belongs to the nuclear hormone receptor family. This receptor is implicated in colon cell differentiation and in colon cancer. Receptor activation by specific agonists has been shown to protect against colon cancer progression. PPARγ protein content within cells is modulated by several mechanisms, including proteasome degradation, activation of Wnt signalling pathways and presence of fermentation products such as butyrate. Herein, we investigated the impact of L-glutamine on PPARγ expression during the differentiation of Caco-2 cells grown in medium containing dialyzed fetal calf serum supplemented or not with L-glutamine. Using RT-PCR and Western blotting, we demonstrated that PPARγ expression was decreased when L-glutamine was added to the medium. Using immunohistochemistry, we demonstrated that PPARγ immunostaining was mainly found in cytoplasm when cells were cultured with L-glutamine while it was found in nuclei and cytoplasm when cells were grown without the addition of L-glutamine. Supershift retardation assays demonstrated a decrease of PPARγ binding onto consensus peroxisome proliferator response element. We concluded that L-glutamine modulated PPARγ expression in Caco-2 cells.

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Influence of storage conditions on normal plasma amino-acid concentrations

Cited by 58 publications

References 21 publications

NMR-based metabonomics of bovine blood: an investigation into the effects of long term storage on plasma samples

NMR-based metabonomics of bovine blood: an investigation into the effects of long term storage on plasma samples

Effects of intravenous arginine on the healing of human skin graft donor sites: A randomized controlled trial

Expression of PPARγ is reduced by medium supplementation with L-glutamine in human colorectal Caco-2 cells

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