NMR-based metabonomics of bovine blood: an investigation into the effects of long term storage on plasma samples

Trabi, Manuela; Keller, Marianne; Jonsson, N.N.

doi:10.1007/s11306-013-0520-2

Cited by 13 publications

(12 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, further testing did not indicate that differences in the metabolomes of matched samples appeared after long-term storage (−80 °C) (Figure 3d–f, Table S3). This is corroborated by previous investigations that tested the influence of storage (−80 °C) duration on metabolite concentrations [24,25]. Previous studies on the impact of long-term (e.g., years) storage on the metabolome have involved relatively short time frames or have not been performed on replicate samples, instead relying on different samples from similar populations, but have shown that storage affected the concentrations of only a few metabolites [26,27].…”

Section: Discussionsupporting

confidence: 89%

Rapid, Reproducible, Quantifiable NMR Metabolomics: Methanol and Methanol: Chloroform Precipitation for Removal of Macromolecules in Serum and Whole Blood

et al. 2018

View full text Add to dashboard Cite

Background: Though blood is an excellent biofluid for metabolomics, proteins and lipids present in blood can interfere with 1d-1H NMR spectra and disrupt quantification of metabolites. Here, we present effective macromolecule removal strategies for serum and whole blood (WB) samples. Methods: A variety of macromolecule removal strategies were compared in both WB and serum, along with tests of ultrafiltration alone and in combination with precipitation methods. Results: In healthy human serum, methanol:chloroform:water extraction with ultrafiltration was compared to methanol precipitation with and without ultrafiltration. Methods were tested in healthy pooled human serum, and in serum from patients with sepsis. Effects of long-term storage at −80 °C were tested to explore the impact of macromolecule removal strategy on serum from different conditions. In WB a variety of extraction strategies were tested in two types of WB (from pigs and baboons) to examine the impact of macromolecule removal strategies on different samples. Conclusions: In healthy human serum methanol precipitation of serum with ultrafiltration was superior, but was similar in recovery and variance to methanol:chloroform:water extraction with ultrafiltration in pooled serum from patients with sepsis. In WB, high quality, quantifiable spectra were obtained with the use of a methanol: chloroform precipitation.

show abstract

Section: Discussionsupporting

confidence: 89%

Rapid, Reproducible, Quantifiable NMR Metabolomics: Methanol and Methanol: Chloroform Precipitation for Removal of Macromolecules in Serum and Whole Blood

et al. 2018

View full text Add to dashboard Cite

show abstract

“…The optimal time is considered to be no more than 35 min and preferably over ice, as longer waiting time may increase lactate levels due to continued glucose metabolism in blood cells [45]. However, limited data are available on long-term storage, although a recent pilot study of heparinized bovine plasma samples stored for up to 15 years reported minimal effects [47]. However, limited data are available on long-term storage, although a recent pilot study of heparinized bovine plasma samples stored for up to 15 years reported minimal effects [47].…”

Section: Pre-analytical Stepsmentioning

confidence: 99%

Metabolomics in hypertension

Nikolic¹,

Sharman²,

Adams³

et al. 2014

Journal of Hypertension

View full text Add to dashboard Cite

Hypertension is the most prevalent chronic medical condition and a major risk factor for cardiovascular morbidity and mortality. In the majority of hypertensive cases, the underlying cause of hypertension cannot be easily identified because of the heterogeneous, polygenic and multi-factorial nature of hypertension. Metabolomics is a relatively new field of research that has been used to evaluate metabolic perturbations associated with disease, identify disease biomarkers and to both assess and predict drug safety and efficacy. Metabolomics has been increasingly used to characterize risk factors for cardiovascular disease, including hypertension, and it appears to have significant potential for uncovering mechanisms of this complex disease. This review details the analytical techniques, pre-analytical steps and study designs used in metabolomics studies, as well as the emerging role for metabolomics in gaining mechanistic insights into the development of hypertension. Suggestions as to the future direction for metabolomics research in the field of hypertension are also proposed.

show abstract

“…Lactate is the most abundant low-weight metabolite we were able to observe in the yak serum. In cattle, its presence has been mainly attributed to ruminal microflora [29]. Its presence in yak serum plays a special role, because its high concentration, together with pyruvate, is a direct consequence of the adaptation to low oxygen levels connected to altitude [30,31,32].…”

Section: Discussionmentioning

confidence: 99%

Characterization of Yak Common Biofluids Metabolome by Means of Proton Nuclear Magnetic Resonance Spectroscopy

Zhu

Wang

et al. 2019

Metabolites

View full text Add to dashboard Cite

The aim of this study was to evaluate the metabolic profiles of yak (Bos grunniens) serum, feces, and urine by using proton nuclear magnetic resonance (1H-NMR), to serve as a reference guide for the healthy yak milieu. A total of 108 metabolites, giving information about diet, protein digestion, and energy generation or gut-microbial co-metabolism, were assigned across the three biological matrices. A core metabolome of 15 metabolites was ubiquitous across all biofluids. Lactate, acetate, and creatinine could be regarded as the most abundant metabolites in the metabolome of serum, feces, and urine, respectively. Metabolic pathway analysis showed that the molecules identified could be able to give thorough information about four main metabolic pathways, namely valine, leucine, and isoleucine biosynthesis; phenylalanine, tyrosine, and tryptophan biosynthesis; glutamine and glutamate metabolism; and taurine and hypotaurine metabolism.

show abstract

NMR-based metabonomics of bovine blood: an investigation into the effects of long term storage on plasma samples

Abstract: Trabi, M., Keller, M.D., and Jonsson, N. (2013)

Cited by 13 publications

References 24 publications

Rapid, Reproducible, Quantifiable NMR Metabolomics: Methanol and Methanol: Chloroform Precipitation for Removal of Macromolecules in Serum and Whole Blood

Rapid, Reproducible, Quantifiable NMR Metabolomics: Methanol and Methanol: Chloroform Precipitation for Removal of Macromolecules in Serum and Whole Blood

Metabolomics in hypertension

Characterization of Yak Common Biofluids Metabolome by Means of Proton Nuclear Magnetic Resonance Spectroscopy

Contact Info

Product

Resources

About