Influence of structure and properties of colloidal biomaterials on cellular uptake and cell functions

Mao, Zhengwei; Zhou, Xiangyan; Gao, Changyou

doi:10.1039/c3bm00137g

Cited by 73 publications

(63 citation statements)

References 195 publications

(234 reference statements)

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“…Using high magnification confocal microscopy and z axis slicing (Figure 5A), we observed that cortical astrocytes and MG cells displayed extensive HALNP co-localization with lysosomes. This high occurrence of co-localization was expected in cortical astrocytes and MG cells because a majority of non-specific uptake mechanisms except caveolae- culminate their endocytosis pathway by fusion with lysosomes [67]. Alternatively, the A172 cells had significantly less HALNP co-localization with lysosomes following intracellular delivery.…”

Section: Resultsmentioning

confidence: 90%

Hyaluronic acid-conjugated liposome nanoparticles for targeted delivery to CD44 overexpressing glioblastoma cells

2016

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Glioblastoma Multiforme (GBM) is a highly prevalent and deadly brain malignancy characterized by poor prognosis and restricted disease management potential. Despite the success of nanocarrier systems to improve drug/gene therapy for cancer, active targeting specificity remains a major hurdle for GBM. Additionally, since the brain is a multi-cell type organ, there is a critical need to develop an approach to distinguish between GBM cells and healthy brain cells for safe and successful treatment. In this report, we have incorporated hyaluronic acid (HA) as an active targeting ligand for GBM. To do so, we employed HA conjugated liposomes (HALNPs) to study the uptake pathway in key cells in the brain including primary astrocytes, microglia, and human GBM cells. We observed that the HALNPs specifically target GBM cells over other brain cells due to higher expression of CD44 in tumor cells. Furthermore, CD44 driven HALNP uptake into GBM cells resulted in lysosomal evasion and increased efficacy of Doxorubicin, a model anti-neoplastic agent, while the astrocytes and microglia cells exhibited extensive HALNP-lysosome co-localization and decreased antineoplastic potency. In summary, novel CD44 targeted lipid based nanocarriers appear to be proficient in mediating site-specific delivery of drugs via CD44 receptors in GBM cells, with an improved therapeutic margin and safety.

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Section: Resultsmentioning

confidence: 90%

Hyaluronic acid-conjugated liposome nanoparticles for targeted delivery to CD44 overexpressing glioblastoma cells

2016

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“…This compound spontaneously forms micron-sized agglomerates (Johnston et al, 2002), subjected to slight size variations after antigen adsorption and in vivo interactions with phosphate, organic acid and proteinaceous environments. A series of recent reports from our laboratory have shown that translocation of aluminium hydroxide may be specifically related to monocyte lineage cell uptake of this poorly biodegradable compound (Khan et al, 2013;Crépeaux et al, 2015;Eidi et al, 2015), likely resulting from phagocytosis or macropinocytosis (Mao et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

“…We previously studied the fate of aluminium particles following im injections. Aluminium hydroxide is a highly hydrated crystalline compound composed of elementary nano-needles of approximately 2.2 nm x 4.5 nm x 10 nm (Mao et al, 2013) and displays a fibrous morphology at transmission electron microscopy (Shirodkar et al, 1990;Eidi et al, 2015). This compound spontaneously forms micron-sized agglomerates (Johnston et al, 2002), subjected to slight size variations after antigen adsorption and in vivo interactions with phosphate, organic acid and proteinaceous environments.…”

Section: Discussionmentioning

confidence: 99%

Non-linear dose-response of aluminium hydroxide adjuvant particles: Selective low dose neurotoxicity

et al. 2017

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Nonlinear dose-response of aluminium hydroxide adjuvant particles: selective low dose neurotoxicity.Toxicology http://dx.doi.org/10.1016/j.tox. 2016.11.018 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. An unusual neuro-toxicological pattern limited to a low dose of Alhydrogel ® was observed.Neurobehavioural changes, including decreased activity levels and altered anxiety-like behaviour, were observed compared to controls in animals exposed to 200 µg Al/kg but not at 400 and 800 µg Al/kg. Consistently, microglial number appeared increased in the ventral forebrain of the 200 µg Al/kg group. Cerebral Al levels were selectively increased in animals exposed to the lowest dose, while muscle granulomas had almost completely disappeared at 6 months in these animals.We conclude that Alhydrogel® injected at low dose in mouse muscle may selectively induce long-term Al cerebral accumulation and neurotoxic effects. To explain this unexpected result, an avenue that could be explored in the future relates to the adjuvant size since the injected suspensions corresponding to the lowest dose, but not to the highest doses, exclusively contained small agglomerates in the bacteria-size range known to favour capture and, presumably, transportation by monocyte-lineage cells. In any event, the view that Alhydrogel® neurotoxicity obeys "the dose makes the poison" rule of classical chemical toxicity appears overly simplistic.

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“…5 Although we have not fully elucidated how the physicochemical properties of MSNs influence their functions to date, it is clear that these biological nanostructures follow design rules. Since 1990s, lots of reports revealed the relationship of MSN size, 6 surface functionalities, [7][8][9] and pore properties 10 11 with the biosystems. In contrast, the focus on particle shape was still relatively low.…”

Section: Introductionmentioning

confidence: 99%

Shape-Mediated Biological Effects of Mesoporous Silica Nanoparticles

Hao¹,

Li²,

Tang³

2014

j biomed nanotechnol

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For biomedical applications, mesoporous silica nanoparticles (MSNs)-based theranostic agents have shown to be a promising alternative. Rational design of particulate systems should consider, beside the physicochemical properties of particle size and surface chemistry, shape features as aspect ratio (AR) and morphology. Recent advances of fabrication technologies for manufacturing different shaped MSNs and evaluation means of its in vitro and in vivo biological performance provide new aspects and wisdom in nanomedicine development. In this review, we discussed the recent progress in the preparation of different shaped MSNs and the evaluation of shape-mediated biological effects. Firstly, we provide an overview of preparation strategies for fabricating MSNs with different aspect ratios and different morphologies, including hollow/rattle MSNs, multishell MSNs, and mesoporous silica nanocomposites. We then highlight the aspect ratio-and morphology-mediated biological effects of MSNs respectively. For AR-mediated biological effects of MSNs, we put our focus in the particle ARs effect on cellular uptake, biocompatibility, and drug delivery. For morphology-mediated biological effects of MSNs, we emphasize on how particle shapes could affect tumor therapy. Finally, for application considerations, we conclude with our personal perspectives on the directions in which future studies in this field might be placed.

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Influence of structure and properties of colloidal biomaterials on cellular uptake and cell functions

Cited by 73 publications

References 195 publications

Hyaluronic acid-conjugated liposome nanoparticles for targeted delivery to CD44 overexpressing glioblastoma cells

Hyaluronic acid-conjugated liposome nanoparticles for targeted delivery to CD44 overexpressing glioblastoma cells

Non-linear dose-response of aluminium hydroxide adjuvant particles: Selective low dose neurotoxicity

Shape-Mediated Biological Effects of Mesoporous Silica Nanoparticles

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