Influence of sunlight on DNA damage in mussels exposed to polycyclic aromatic hydrocarbons

Steinert, Scott; Montee, R.S.; Sastre, Miguel P.

doi:10.1016/s0141-1136(97)00088-3

Cited by 32 publications

(10 citation statements)

References 7 publications

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“…Additionally, a nonspecific response associated with disruption in the lipid component of cellular membranes that reflects exposure and toxicity to pollut--ants was assessed through lipid peroxidation (LPO) determined in the gills (Cajaraville et al, 2000;Viarengo et al, 2007). Genotoxic potential of contaminants was also evaluated due to their ability to interfere with DNA integrity (Steinert et al, 1998) analysing DNA damage in clam's haemolymph with the alkaline single--cell gel electrophoresis (Comet) assay. Finally, estrogenic contamination was assessed through the effect of xenoestrogens, vitellogenin induction mainly in males and immature females, applying the indirect alkali--labile phosphate (ALP) method to clam's gonads (Matozzo et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

A multibiomarker approach in the clam Ruditapes decussatus to assess the impact of pollution in the Ria Formosa lagoon, South Coast of Portugal

Cravo

Pereira

Gomes

et al. 2012

Marine Environmental Research

107

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Section: Introductionmentioning

confidence: 99%

A multibiomarker approach in the clam Ruditapes decussatus to assess the impact of pollution in the Ria Formosa lagoon, South Coast of Portugal

Cravo

Pereira

Gomes

et al. 2012

Marine Environmental Research

107

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“…Though, most of the nuclear buds have different origin than micronuclei, studies in human tumor cells have indicated, that in the S-phase of the cell cycle amplified chromosomal or double minute DNA may form buds, later disintegrate from the nucleus and become a micronucleus (Pedeutour et al, 1994, Shimizu et al, 1998, 2000, Yankiwski et al, 2000. Fragmentation of cells and apoptosis can be provoked by a variety of factors, including those with genotoxic potential (Steinert, 1996;Steinert et al, 1998;Mičić et al, 2001;Baršienė et al, 2005;, 2006d, or directly associated to cytotoxic events (Bolognesi et al, 2006b). Data on other nuclear abnormalities presents a wider view on cellular processes following the exposure and permits the measurement of important complementary genotoxicity and cytotoxicity events.…”

Section: Introductionmentioning

confidence: 99%

Environmental genotoxicity and cytotoxicity in the offshore zones of the Baltic and the North Seas

Rybakovas

Baršienė

Lang

2009

Marine Environmental Research

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Please cite this article as: Rybakovas, A., Baršienė, J., Lang, T., Environmental genotoxicity and cytotoxicity in the offshore zones of the Baltic and North Seas, Marine Environmental Research (2009Research ( ), doi: 10.1016Research ( /j.marenvres. 2009 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. ACCEPTED MANUSCRIPT AbstractMicronuclei (MN), nuclear buds (NB) and fragmented-apoptotic cells (FA) were analyzed in mature peripheral blood and immature cephalic kidney erythrocytes of flounder (Platichthys flesus), dab (Limanda limanda) and cod (Gadus morhua) from 12 offshore sites in the Baltic Sea (479 specimens) and 11 sites in the North Sea (291 specimens), which were collected during three research vessel cruises in December 2002 and in September 2004. The highest levels of environmental genotoxicity (frequencies of MN up to 0.5 ‰, NB -up to 0.75 ‰) and cytotoxicity (FA -up to 0.53 ‰) were observed in flatfishes from areas close to oil and gas platforms in the North Sea and in zones related to the extensive shipping and potentially influenced by contamination from large European Rivers (Elbe, Vistula, Oder). In dab from the offshore zones of the North Sea, the levels of nuclear abnormalities were higher as compared to those in dab from the Baltic Sea. Responses in immature kidney erythrocytes were higher than in mature erythrocytes from peripheral blood. MN frequency lower than 0.05 ‰ (the Baltic Sea) and lower than 0.1 ‰ (the North Sea) could be suspected as a reference level in the peripheral blood erythrocytes of flatfish.Keywords: Genotoxicity; Cytotoxicity; Micronuclei; Nuclear abnormalities; Erythrocytes; Baltic Sea; North Sea. ACCEPTED MANUSCRIPT 2 IntroductionSurrounded by European countries, the Baltic and the North Seas are constantly exposed to anthropogenic pressure. With pollution from land-based discharges, coastal industries, municipal outflows, agricultural activities, oil and gas industry (drilling and transportation) and transport activities, thousands of chemicals are entering the marine environment. A large proportion of these compounds are potentially toxic, genotoxic and carcinogenic to aquatic organisms (Jha, 2004(Jha, , 2008. The interaction of genotoxic contaminants with DNA causes various genetic disturbances, which often are irreversible and can be transmitted to the next generations (Depledge and Fossi, 1994;Dixon et al., 1999;Jha, 2004). The analysis of environmental genotoxicity provides early warning signs of adverse long-term effects of contamination. A number of biomarkers have been applied for assessment of environmental genotoxicity and the micronuclei (MN) test,...

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“…Genotoxic substances may react directly with DNA, or following biotransformation to reactive oxygen species or other reactive intermediates. Several factors may influence the interaction, such as ambient sunlight, inducing phototoxicity (Mitchelmore, Birmelin, Chipman, & Livingstone, 1998;Steinert, Streib Montee, & Sastre, 1998). For instance, a reduction in the DNA strand breaks in oysters (Crassostrea virginica) following field deployment and then allowed to recover in the laboratory for 7-10 days has been reported, indicating the reversibility and non persistence of such damage (Nacci, S. Nelson, W. Nelson, & Jackim, 1992).…”

Section: Discussionmentioning

confidence: 99%

Monitoring of Organic Compounds and Trace Metals During a Dredging Episode in the Göta Älv Estuary (SW Sweden) Using Caged Mussels

Bellas

Ekelund²,

Halldórsson

et al. 2006

Water Air Soil Pollut

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The concentrations of selected trace metals, organotins, polychlorinated biphenyls (PCBs), and polycyclic aromatic hydrocarbons (PAHs) were determined in caged blue mussels (Mytilus edulis) during dredging operations in the Göta Älv Estuary (SW Sweden). Maximum values of pollutants in mussel tissues were found after the dredging started. Our results showed that the dredging caused mobilization of organotins from the sediments to the water column during the experimental period. Multidimensional scaling (MDS) and cluster analysis were applied to compare and establish relationships between levels of pollutants in mussels and sampling sites during the experimental period. In order to evaluate the biological effects of contaminants, genotoxic damage was measured using the Comet assay, and its potential application for environmental monitoring is discussed.

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Influence of sunlight on DNA damage in mussels exposed to polycyclic aromatic hydrocarbons

Cited by 32 publications

References 7 publications

A multibiomarker approach in the clam Ruditapes decussatus to assess the impact of pollution in the Ria Formosa lagoon, South Coast of Portugal

A multibiomarker approach in the clam Ruditapes decussatus to assess the impact of pollution in the Ria Formosa lagoon, South Coast of Portugal

Environmental genotoxicity and cytotoxicity in the offshore zones of the Baltic and the North Seas

Monitoring of Organic Compounds and Trace Metals During a Dredging Episode in the Göta Älv Estuary (SW Sweden) Using Caged Mussels

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