Influence of temperature on Salmonella survival in hog manure slurry and seasonal temperature profiles in farm manure storage reservoirs

Arrus, Katia M.; Holley, Richard A.; Ominski, Kim; Tenuta, Mario; Blank, G.

doi:10.1016/j.livsci.2006.03.021

Cited by 54 publications

(28 citation statements)

References 35 publications

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“…Temperature has been shown to be an important factor in the survival of pathogenic bacteria in environmental samples (1,22,36). In our study, significant differences in Salmonella serovar Typhimurium survival at different temperatures were found by using three different detection methods.…”

Section: Discussionmentioning

confidence: 64%

Influence of Temperature and Predation on Survival of Salmonella enterica Serovar Typhimurium and Expression of invA in Soil and Manure-Amended Soil

Bælum

Fredslund

et al. 2010

Appl Environ Microbiol

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The effects of three temperatures (5, 15, and 25°C) on the survival of Salmonella enterica serovar Typhimurium in topsoil were investigated in small microcosms by three different techniques: plate counting, invA gene quantification, and invA mRNA quantification. Differences in survival were related to the effect of protozoan predation. Tetracycline-resistant Salmonella serovar Typhimurium was inoculated into soil and manure-amended soil at 1.5 ؋ 10 8 cells g soil ؊1 . Population densities were determined by plate counting and by molecular methods and monitored for 42 days. Simultaneous extraction of RNA and DNA, followed by quantitative PCR, was used to investigate invA gene levels and expression. Analysis by these three techniques showed that Salmonella serovar Typhimurium survived better at 5°C. Comparing DNA and CFU levels, significantly higher values were determined by DNA-based techniques. invA mRNA levels showed a fast decrease in activity, with no detectable mRNA after an incubation period of less than 4 days in any of the soil scenarios. A negative correlation was found between Salmonella serovar Typhimurium CFU levels and protozoan most probable numbers, and we propose the role of the predator-prey interaction as a factor to explain the die-off of the introduced strain by both culture-and DNA quantification-based methods. The results indicate that temperature, manure, and protozoan predation are important factors influencing the survival of Salmonella serovar Typhimurium in soil.

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Section: Discussionmentioning

confidence: 64%

Influence of Temperature and Predation on Survival of Salmonella enterica Serovar Typhimurium and Expression of invA in Soil and Manure-Amended Soil

Bælum

Fredslund

et al. 2010

Appl Environ Microbiol

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“…According to these authors, the observed order of destruction makes it possible to predict storage conditions (temperature and time) that will lead to a predetermined level of reduction of the two pathogens. Arrus et al (2006) observed influence of temperature on S. typhimurium and another three Salmonella serovars in hog slurry from different production phases stored at 4 °C, 25°C or 37 °C. Salmonella survived for > 300 days at 4°C.…”

Section: Resultsmentioning

confidence: 85%

The risk to human health related to disposal of animal wastes to soil — microbiological and parasitical aspects

et al. 2013

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SummaryThe study was conducted to investigate the microbiological and parasitical risk related to the disposal of animal manure to soil by storage of raw pig slurry at temperatures 4°C, 20 °C and 42 °C for 115 days. Plate counts of Salmonella typhimurium and number of devitalized non-embryonated model Ascaris suum eggs were determined on days 0, 7, 12, 22, 32, 40, 55, 90 and 115 of storage. At the same intervals level of selected physico-chemical parameters were determined. Microbiological examination showed that S. typhimurium survived in the slurry for less than 115 days at 4 °C and less than 90 days at 20 °C and 42 °C. Devitalization of A. suum eggs increased with temperature and time of storage, but complete devitalization was not achieved even after 115 days at 42 °C. Physico-chemical parameters showed changes related to decomposition processes, but did not allow us to draw definite conclusion regarding their influence on devitalization of pathogens. The results indicate potential risk to human food chain that can be prevented by strict observation of legislative provisions and appropriate treatment of animal manure.

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“…In general, temperature has an important efect on growth and decay rates of bacteria. Most studies examined the inluence of temperature on survival of enteric bacteria under isothermal conditions, showing a generally reduced survival of Salmonella in soil with increasing temperature and, accordingly a beter persistence in soil at lower temperatures [36,37]. Semenov et al [38] analyzed how temperature luctuations afect Salmonella enterica serovar Typhimurium (S. Typhimurium) in cow manure and demonstrated increased decay rates with increasing amplitudes of daily oscillations.…”

Section: Factors Inluencing the Survival Of Salmonella In Soilmentioning

confidence: 99%

Interaction between Salmonella and Plants: Potential Hosts and Vectors for Human Infection

Fornefeld¹,

Schierstaedt²,

Jechalke³

et al. 2017

Current Topics in Salmonella and Salmonellosis

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Fruits and vegetables are important for a healthy diet. However, when eaten raw and contaminated with human pathogens (HPs) they may cause a disease outbreak. Contamination with HPs can occur along the entire farm-to-fork production chain and Salmonella enterica is one of the most common foodborne pathogens. A range of biotic and abiotic environmental factors can inluence the complex interactions between Salmonella and plants. Moreover, the outcome of experiments largely depends on the experimental design and parameters or methods employed, and on top, on the accompanying plant microbiome and the genetic equipment of the plant and the Salmonella strain. Particularly mobile genetic elements contribute to the diversiication and adaptation of Salmonella to the plant environment. So far, litle is known about the key processes and factors inluencing the atachment and potential internalization of Salmonella in plants and the plant speciic responses. It is therefore important to beter understand the ecology of Salmonella in the soil and plant environment, in order to propose practicable recommendations for prevention of foodborne diseases. This also requires improved sensitivity and speciicity of detection methods. In this chapter, we present the current knowledge, research needs, and methodology regarding the complex interactions between Salmonella and plants.

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Influence of temperature on Salmonella survival in hog manure slurry and seasonal temperature profiles in farm manure storage reservoirs

Cited by 54 publications

References 35 publications

Influence of Temperature and Predation on Survival of Salmonella enterica Serovar Typhimurium and Expression of invA in Soil and Manure-Amended Soil

Influence of Temperature and Predation on Survival of Salmonella enterica Serovar Typhimurium and Expression of invA in Soil and Manure-Amended Soil

The risk to human health related to disposal of animal wastes to soil — microbiological and parasitical aspects

Interaction between Salmonella and Plants: Potential Hosts and Vectors for Human Infection

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