Influence of temperature on the progamic phase in Citrus

Montalt, Rafael; Cuenca, José; Vives, Marı́a C.; Navarro, Luís; Ollitrault, Patrick; Aleza, Pablo

doi:10.1016/j.envexpbot.2019.103806

Cited by 14 publications

(6 citation statements)

References 71 publications

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“…For that purpose, pistils were submerged 3 times in water for 1 h. Afterward, pistils were sliced into 14 cross-sections using a sharp blade. Stigmas were sliced into two sections, styles into eight sections, and ovaries into four sections following the methodology described by Montalt et al [44]. Sections were stained with 0.1% aniline blue in 0.1 N K 3 PO 4 [45].…”

Section: Histological Observationsmentioning

confidence: 99%

“…Male and female gametophytes (from meiosis to zygote formation) are exposed plant structures, which potentially makes them especially susceptible to environmental conditions [68,69]. Temperature is one of the main environmental conditions that influences the success of plant sexual reproduction [70,71] and several studies report the impact of temperature on gametophytic generation and the progamic phase [44,69,[72][73][74][75]. To the best of our knowledge, influence of environmental conditions in PA is unnoticed in citrus.…”

Section: Parthenocarpic Classificationmentioning

confidence: 99%

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Parthenocarpy and Self-Incompatibility in Mandarins

et al. 2021

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Citrus reproductive biology is complex. One of its characteristic features is parthenocarpy that enables seedless fruit production. Citrus parthenocarpy and self-incompatibility knowledge is only partial and sometimes discrepant. Increasing such knowledge is relevant for better managing cultivated varieties and improving the selection of parents in breeding strategies to recover seedless varieties such as mandarins. This work develops an efficient protocol to characterize self-incompatibility and different parthenocarpy types based on emasculation, hand self-pollination, and hand cross-pollination. It analyzes fruit setting and seed production coupled with histological pollen performance observations. We analyzed the reproductive behavior of nine mandarin varieties with relevant characteristics as parents for seedless mandarin breeding. ‘Clemenules’ clementine and ‘Moncada’ mandarins were strictly self-incompatible with facultative and vegetative parthenocarpy; ‘Imperial’ mandarin and ‘Ellendale’ tangor displayed no strict self-incompatibility associated with facultative and vegetative parthenocarpy; ‘Fortune’ mandarin was self-incompatible with facultative and stimulative parthenocarpy; ‘Campeona’ and ‘Salteñita’ mandarins were self-compatible with vegetative parthenocarpy; ‘Serafines’ satsuma was associated with male sterility together with facultative and vegetative parthenocarpy; and ‘Monreal’ clementine was self-compatible and nonparthenocarpic. Our protocol can be applied for screening of mandarin germplasm and to characterize new parents. Reproductive behavior knowledge is important for optimizing seedless mandarin breeding programs based on diploidy, triploidy, or induced mutagenesis.

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Section: Histological Observationsmentioning

confidence: 99%

Section: Parthenocarpic Classificationmentioning

confidence: 99%

Parthenocarpy and Self-Incompatibility in Mandarins

et al. 2021

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“…In the immediate following days (1 to 2 days at 30 • C and 1 to 5 days at 10 • C), 25 flowers at anthesis for each temperature regime were used to perform hand-pollination; ten flowers were used for histological observations (Supplementary Table S1), and 15 flowers were left for fruit setting. According to our previous results about the influence of temperature on the citrus progamic phase [64], the time between pollination and FAA fixation differed depending on the temperature regime under study. At 10 • C, flowers were fixed 20 days after pollination, whereas at 30 • C, flowers were fixed 4 days after pollination.…”

Section: Methodsmentioning

confidence: 80%

“…The pistils fixed in FAA were submerged 3 times in water for 1 h. Then, pistils were sliced into 14 cross-sections using a sharp blade. Stigmas were sliced into two sections, styles into eight sections and ovaries into four sections, following the methodology described by Montalt et al [64]. Sections were stained with 0.1% aniline blue in 0.1 N K 3 PO 4 [71].…”

Section: Histological Observationsmentioning

confidence: 99%

“…Previous studies in citrus have demonstrated that temperature affects not only pollen grain germination but also pollen tube growth dynamics and kinetics [62][63][64]. In selfincompatible genotypes like clementines, pollen tube growth is arrested in the first third of the style at high temperatures (25-30 • C), whereas very few pollen tubes reach ovaries at low temperatures (15-20 • C) [63].…”

Section: Introductionmentioning

confidence: 99%

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Breakdown of Self-Incompatibility in Citrus by Temperature Stress, Bud Pollination and Polyploidization

et al. 2022

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Self-incompatibility (SI) is present in around half of all species of flowering plants. SI limits endogamy and contributes to increased genetic diversity. SI is a very important trait in citrus because, when coupled with parthenocarpy, it allows seedless fruit production. Otherwise, SI is an impediment to genetic studies and breeding programs. Temperature stress, bud pollination and polyploidization can induce the breakdown of the SI mechanism in several species. In this work, we investigated how the SI mechanism can be broken down in two self-incompatible diploid citrus genotypes: ‘Fortune’ mandarin and ‘Clemenules’ clementine. The influence of temperature stress on the SI mechanism was assessed in self-pollinated flowers of ‘Fortune’ mandarins subjected to 2 temperature regimes (10 °C and 30 °C), whereas the bud pollination effect was investigated in the same genotype and in ‘Clemenules’ clementines cultivated under field conditions. The tetraploid ‘Clemenules’ clementine cultivated under field conditions was used to study if tetraploidization can bypass the SI reaction. Histological observations of pollen tube growth and seed production in self-pollinated flowers were used to evaluate the breakdown of SI, while the genetic analysis with SSR and SNP markers confirmed that all recovered plants were zygotic and had been originated by selfing. Our results confirm that the SI reaction can be surpassed by temperature stress, bud pollination and tetraploidy. To our knowledge, this is the first report in citrus in which the SI reaction breakdown by these three different strategies is demonstrated by molecular markers.

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Putrescine Affects Fruit Yield and Quality by Promoting Effective Pollination Period in Citrus limon

Karabıyık

2024

Applied Fruit Science

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Influence of temperature on the progamic phase in Citrus

Cited by 14 publications

References 71 publications

Parthenocarpy and Self-Incompatibility in Mandarins

Parthenocarpy and Self-Incompatibility in Mandarins

Breakdown of Self-Incompatibility in Citrus by Temperature Stress, Bud Pollination and Polyploidization

Putrescine Affects Fruit Yield and Quality by Promoting Effective Pollination Period in Citrus limon

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