Influence of the operating conditions and the external mass transfer limitations on the synthesis of fatty acid esters using a Candida antarctica lipase

Trubiano, G.; Borio, Daniel Oscar; Errazu, A.F.

doi:10.1016/j.enzmictec.2006.06.003

Cited by 48 publications

(47 citation statements)

References 22 publications

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“…Denaturation of lipase caused by polar substrate (alcohol) was also observed elsewhere [8,17], suggesting a stepwise addition of ethanol to avoid lipase deactivation due to the high initial concentration of alcohol. As shown in Fig.…”

Section: Fatty Acid Alcohol Ester Watermentioning

confidence: 85%

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Biodiesel Synthesis via Esterification of Feedstock with High Content of Free Fatty Acids

Souza

Aguieiras

Silva

et al. 2008

Appl Biochem Biotechnol

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The objective of this work was to study the synthesis of ethyl esters via esterification of soybean oil deodorizer distillate with ethanol, using solid acid catalysts and commercial immobilized lipases, in a solvent-free system. Three commercially immobilized lipases were used, namely, Lipozyme RM-IM, Lipozyme TL-IM, and Novozym 435, all from Novozymes. We aimed for optimum reaction parameters: temperature, enzyme concentration, initial amount of ethanol, and its feeding technique to the reactor (stepwise ethanolysis). Reaction was faster with Novozym 435. The highest conversion (83.5%) was obtained after 90 min using 3 wt.% of Novozym 435 and two-stage stepwise addition of ethanol at 50 degrees C. Four catalysts were also tested: zeolite CBV-780, SAPO-34, niobia, and niobic acid. The highest conversion (30%) was obtained at 100 degrees C, with 3 wt.% of CBV-780 after 2.5 h. The effects of zeolite CBV 780 concentration were studied, resulting in a conversion of 49% using 9 wt.% of catalyst.

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Section: Fatty Acid Alcohol Ester Watermentioning

confidence: 85%

“…Esterification reactions between alcohols and free fatty acids can also be catalyzed by lipases in non-aqueous or microaqueous media. The production of esters can be achieved either by synthesis with free fatty acids and alcohols or by transesterification [6][7][8][9].…”

Section: Introductionmentioning

confidence: 99%

Biodiesel Synthesis via Esterification of Feedstock with High Content of Free Fatty Acids

Souza

Aguieiras

Silva

et al. 2008

Appl Biochem Biotechnol

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“…The reaction was performed at various agitation speeds and for different time periods generated by RSM, as indicated in Table 1. The initial rates were calculated from the time profiles corresponding to the first minutes of reaction (for which the profiles were approximately linear) and expressed as the amount of acid converted per unit of time per unit of weight of enzyme [20].…”

Section: Esterification Reactionmentioning

confidence: 99%

Optimized lipase-catalyzed synthesis of adipate ester in a solvent-free system

Chaibakhsh

Rahman

Abd‐Aziz³

et al. 2009

J Ind Microbiol Biotechnol

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Immobilized Candida antarctica lipase-catalyzed esterification of adipic acid and oleyl alcohol was investigated in a solvent-free system (SFS). Optimum conditions for adipate ester synthesis in a stirred-tank reactor were determined by the response surface methodology (RSM) approach with respect to important reaction parameters including time, temperature, agitation speed, and amount of enzyme. A high conversion yield was achieved using low enzyme amounts of 2.5% w/w at 60 degrees C, reaction time of 438 min, and agitation speed of 500 rpm. The good correlation between predicted value (96.0%) and actual value (95.5%) implies that the model derived from RSM allows better understanding of the effect of important reaction parameters on the lipase-catalyzed synthesis of adipate ester in an organic solvent-free system. Higher volumetric productivity compared to a solvent-based system was also offered by SFS. The results demonstrate that the solvent-free system is efficient for enzymatic synthesis of adipate ester.

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“…It was observed that yield of ethyl ester enhanced with increase in reaction temperature from 25 41 ethyl ester to 35 80.5 ethyl ester . Further increase in the temperature from 45 62 ethyl ester to 65 18 ethyl ester resulted in sharp decrease in the yield of ethyl ester Fig.…”

Section: Effect Of Temperaturementioning

confidence: 99%