Influence of the particle size distribution of the packing material in reversed-phase high-performance liquid chromatography

Dewaele, C.; Verzele, M.

doi:10.1016/0021-9673(83)80002-8

Cited by 54 publications

(21 citation statements)

References 15 publications

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“…Zirconia is stable in a very wide pH range (pH [1][2][3][4][5][6][7][8][9][10][11][12][13][14], but zirconia surface has relatively low reactivity (more difficult to bond different functional groups to the surface), which significantly limits a selection of available stationary phases.…”

Section: Base Materials (Silica Zirconia Alumina Polymers)mentioning

confidence: 99%

“…The narrower the particle size distribution, the better and the more reproducibly the columns could be packed. Generally accepted criteria is that 95% of all particles should be within 25% region around the mean particle diameter [11,12].…”

Section: Particle Size Distributionmentioning

confidence: 99%

“…This type of reaction allows for a very high bonding density, but hydrolytic cleavage of Si-O-R bonds is relatively high and this material is not practically applicable for HPLC support. Chemical modification of the silica surface with hydridosilane-type modifiers as shown in schematic (3-11) offers some advantages: (3)(4)(5)(6)(7)(8)(9)(10)(11) A byproduct of this reaction is H 2 , which easily leaves the reaction zone and leads to higher bonding density [18]. …”

Section: Types Modification Reactionsmentioning

confidence: 99%

“…Vapor-phase silanization reactions with rigorous control of the absence of water show that Cl n SiMe 4 -n-type chlorosilanes react with aerosil (nonporous microparticulate silica) as (3)(4)(5)(6)(7)(8)(9)(10)(11)(12) that is, only one surface silanol participates in this reaction [54]. Unger [6], on the other hand, advocates the possibility of the formation of bridging bonds:…”

Section: Surface Modification With Chlorosilanesmentioning

confidence: 99%

“…Bonding density is calculated using the value of carbon content (from CHN analysis) in carbon %w/w and the specific surface area, obtained from the BET analysis of base silica. Calculation is performed using the equation originally introduced by Berendsen and de Galan [68]: (3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14) where C% is the carbon percent by weight, n c is the number of carbons in bonded ligand, MW is the molecular weight of bonded ligand, and S is the silica surface area. This expression is only applicable for monomeric bonding.…”

Section: Density Of Bonded Ligandsmentioning

confidence: 99%

See 4 more Smart Citations

Stationary Phases

Kazakevich¹,

LoBrutto²

2006

HPLC for Pharmaceutical Scientists

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Section: Base Materials (Silica Zirconia Alumina Polymers)mentioning

confidence: 99%

Section: Particle Size Distributionmentioning

confidence: 99%

Section: Types Modification Reactionsmentioning

confidence: 99%

Section: Surface Modification With Chlorosilanesmentioning

confidence: 99%

Section: Density Of Bonded Ligandsmentioning

confidence: 99%

See 3 more Smart Citations

Stationary Phases

Kazakevich¹,

LoBrutto²

2006

HPLC for Pharmaceutical Scientists

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Die Chromatographie, eine zentrale Methode in der biotechnischen Aufarbeitung

Müller

Brümmer

1990

Chemie Ingenieur Technik

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Hydrophilic interaction electrokinetic chromatography using bio‐based nanofillers

et al. 2014

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Hydrophilic interaction (HI)-based separation like HILIC is effective for analyzing hydrophilic biological samples such as carbohydrates, peptides, and metabolites. To overcome the drawbacks of conventional HILIC such as large consumption of organic solvents and easy deterioration of the separation column, we developed HI electrokinetic chromatography (EKC) by employing bio-based nanomaterials as the hydrophilic pseudostationary phase. By mechanical/chemical treatments, cellulose, chitin, and chitosan were processed to 10-nm wide nanofibers/nanowhiskers (NFs/NWs), which are longer/shorter than 1000/200 nm, respectively. In HI-EKC of oligosaccharides using 0.001% uncharged cellulose NFs, strong interaction was observed for the large-size oligosaccharides with the retention factors (k) of up to 1.56, indicating a HILIC-mode interaction. In HI-EKC with 0.1% positively charged chitosan NFs, benzenedisulfonic acid, benzenesulfonic acid (BS), and p-hydroxy BS (HBS) had k values of 0.036, 0.018, and 0.018, respectively, suggesting that the ion-exchange interaction mainly occurred via sulfonate groups. Finally, HI-EKC was demonstrated using 0.05% chitin or chitosan NWs. In both cases using chitin and chitosan NWs, HBS showed much stronger interaction with k > 0.192 compared with BS with k < 0.070. It indicated structural difference between NFs and NWs affected the HI behavior in terms of both the ion-exchange and HILIC modes.

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Influence of the particle size distribution of the packing material in reversed-phase high-performance liquid chromatography

Cited by 54 publications

References 15 publications

Stationary Phases

Stationary Phases

Die Chromatographie, eine zentrale Methode in der biotechnischen Aufarbeitung

Hydrophilic interaction electrokinetic chromatography using bio‐based nanofillers

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