The conventional approach to qualitative and quantitative analyses of hemoglobin (Hb) molecules for the diagnoses of hemoglobinopathies requires a combination of tests. We used an automated HPLC (VARIANT™) system to study α-thalassemia and β-thalassemia syndromes in Thailand. The beta-thalassemia short program is applicable to the diagnosis of α-thalassemia and β-thalassemia disorders, including Hb H, EA Bart’s disease, and EF Bart’s disease, in adults, newborns, and fetuses. The system cannot quantify accurately certain Hb molecules, such as Hb H and Hb Bart’s. The alpha-thalassemia short program was therefore developed and used to quantify Hb Bart’s to detect α-thalassemia genotypes in cord blood. This automated HPLC system is an alternative approach to the diagnosis of complicated thalassemia syndromes in Thailand and Southeast Asia.

No abstract

No abstract

No abstract

No abstract