Influence of the plant growing conditions on the translocation routes and systemic infection of carnation mottle virus in Chenopodium quinoa plants

García-Castillo, Silvia; Marcos, José F.; Pallás, Vicente; Sánchez-Pina, M. Amelia

doi:10.1006/pmpp.2001.0332

Cited by 19 publications

(11 citation statements)

References 43 publications

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“…) and incubated at 37°C for 30–60 min, followed by a water rinse to stop the reaction (Garcia‐Castillo et al. ). The stained tissues were dehydrated through an ethanol series, each for 5 min (30, 50, 70, 95 and two times 100%), then immersed in Hemo‐De two times for 5 min each and mounted on slides using Permount.…”

Section: Methodsmentioning

confidence: 99%

Recovery from Cucumber Mosaic Virus Infection for ‘Calwonder’ Bell Pepper Plants Does not Counter Negative Impacts on Plant Growth

Murphy

Masiri

Hadi

et al. 2015

Journal of Phytopathology

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Cucumber mosaic virus (CMV) accumulation in leaves and stems of infected bell pepper plants at specific symptom stages was evaluated with an emphasis on the transition from full infection to recovery from Cucumber mosaic disease. Four symptom phases occurred in successive order, designated chlorosis (leaves 6-8), mosaic (leaves 9-11/12), leaf distortion (first series of leaves on secondary and tertiary branches) and recovery (progressive recovery with newly emerging leaves in tertiary and younger branches). In situ detection of CMV in leaf tissues revealed widespread occurrence in leaves expressing chlorosis and mosaic symptoms but reduced, localized occurrence in leaves in the recovery phase. Similarly, CMV accumulated to high levels throughout stems expressing chlorosis and mosaic symptoms but with dramatically reduced levels for plants in the recovery symptom phase. Stunting of internodes occurred at all locations above the inoculated leaves by the first expression of systemic symptoms, suggesting an impact on stem growth in response to initial virus invasion of young developing tissues of the stem. Despite the recovery from CMV infection, plant growth was negatively impacted early in the infection process and remained so through the course of the experiment.

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Section: Methodsmentioning

confidence: 99%

Recovery from Cucumber Mosaic Virus Infection for ‘Calwonder’ Bell Pepper Plants Does not Counter Negative Impacts on Plant Growth

Murphy

Masiri

Hadi

et al. 2015

Journal of Phytopathology

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“…Ten flower buds, ovaries and seeds were fixed by immersion in a freshly made mixture of 4 % paraformaldehyde and 2.5 % glutaraldehyde in 0.1 M phosphate buffer (pH 7.2), at 4 uC for 4 h and embedded in paraffin (Paraplast Plus) as described previously (García-Castillo et al, 2001;Amari et al, 2007). The same probe used for dot-blot hybridization was used for in situ hybridization.…”

Section: Methods Plant Material Apricot Trees (Prunus Armeniaca L) mentioning

confidence: 99%

“…In situ hybridization was performed at 55 uC overnight. Slide pre-treatments, hybridization and colourigenic detection were carried out as described previously (García-Castillo et al, 2001;Amari et al, 2007). Sections were examined and photographed with a Leica DC 500 digital camera using a DMRB Leitz microscope.…”

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confidence: 99%

Vertical transmission of Prunus necrotic ringspot virus: hitch-hiking from gametes to seedling

Amari

Burgos

Pallás

et al. 2009

Journal of General Virology

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The aim of this work was to follow Prunus necrotic ringspot virus (PNRSV) infection in apricot reproductive tissues and transmission of the virus to the next generation. For this, an analysis of viral distribution in apricot reproductive organs was carried out at different developmental stages. PNRSV was detected in reproductive tissues during gametogenesis. The virus was always present in the nucellus and, in some cases, in the embryo sac. Studies within infected seeds at the embryo globular stage revealed that PNRSV infects all parts of the seed, including embryo, endosperm and testa. In the torpedo and bent cotyledon developmental stages, high concentrations of the virus were detected in the testa and endosperm. At seed maturity, PNRSV accumulated slightly more in the embryo than in the cotyledons. In situ hybridization showed the presence of PNRSV RNA in embryos obtained following hand-pollination of virus-free pistils with infected pollen. Interestingly, tissue-printing from fruits obtained from these pistils showed viral RNA in the periphery of the fruits, whereas crosses between infected pistils and infected pollen resulted in a total invasion of the fruits. Taken together, these results shed light on the vertical transmission of PNRSV from gametes to seedlings.

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“…Different organs of both healthy and MNSV‐infected melon plants, including cotyledons, young and developed leaves and their corresponding petioles, roots and stems, as well as shoot tips were sampled (from 4 to 20 dpi) and then fixed. Briefly, samples were vacuum‐infiltrated for 2 min in a freshly made mixture of 4% p ‐formaldehyde and 2.5% glutaraldehyde in 0.2 m phosphate buffer (pH 7.2) at 4 °C for 4 h, essentially as described (García‐Castillo et al ., 2001, 2003). After fixation, they were washed in 0.2 m phosphate buffer (pH 7.2), dehydrated in a tertiary butyl alcohol series, infiltrated and embedded in paraffin (Paraplast Plus, Sherwood Medical Co., St Louis, MO).…”

Section: Methodsmentioning

confidence: 99%

“…In situ hybridization (ISH) was performed as previously described (García‐Castillo et al ., 2001, 2003, Más et al ., 2000). A total of 17 ISH experiments were performed, which included, in each experiment, slides containing sections from all the organs analysed (cotyledons, leaves, petioles, stems, roots and shoot tips), and from the different time‐points studied.…”

Section: Methodsmentioning

confidence: 99%

Distribution and pathway for phloem‐dependent movement of Melon necrotic spot virus in melon plants

Gosalvez‐Bernal

Genovés

Navarro

et al. 2008

Molecular Plant Pathology

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The translocation of Melon necrotic spot virus (MNSV) within tissues of inoculated and systemically infected Cucumis melo L. 'Galia' was studied by tissue-printing and in situ hybridization techniques. The results were compatible with the phloem vascular components being used to spread MNSV systemically by the same assimilate transport route that runs from source to sink organs. Virus RNAs were shown to move from the inoculated cotyledon toward the hypocotyl and root system via the external phloem, whereas the upward spread through the stem to the young tissues took place via the internal phloem. Virus infection was absent from non-inoculated source tissues as well as from both shoot and root apical meristems, but active sink tissues such as the young leaves and root system were highly infected. Finally, our results suggest that the MNSV invasion of roots is due to virus replication although a destination-selective process is probably necessary to explain the high levels of virus accumulation in roots. This efficient invasion of the root system is discussed in terms of natural transmission of MNSV by the soil-borne fungal vector.

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Influence of the plant growing conditions on the translocation routes and systemic infection of carnation mottle virus in Chenopodium quinoa plants

Cited by 19 publications

References 43 publications

Recovery from Cucumber Mosaic Virus Infection for ‘Calwonder’ Bell Pepper Plants Does not Counter Negative Impacts on Plant Growth

Recovery from Cucumber Mosaic Virus Infection for ‘Calwonder’ Bell Pepper Plants Does not Counter Negative Impacts on Plant Growth

Vertical transmission of Prunus necrotic ringspot virus: hitch-hiking from gametes to seedling

Distribution and pathway for phloem‐dependent movement of Melon necrotic spot virus in melon plants

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