Vertical transmission of Prunus necrotic ringspot virus: hitch-hiking from gametes to seedling

Amari, Khalid; Burgos, L.; Pallás, Vicente; Sánchez-Pina, M. Amelia

doi:10.1099/vir.0.009647-0

Cited by 51 publications

(24 citation statements)

References 44 publications

(47 reference statements)

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“…ALSV in pollen and embryo sacs might therefore be inactivated during fertilization and/or seed maturation. In apricot infected with PNRSV, not all pollen grains were infected, but the virus was always present in the nucellus and sometimes present in the embryo sac of female reproductive organs (Amari et al 2007(Amari et al , 2009). In contrast, in apple, ALSV was detected in most pollen grains and in all embryo sacs, but barely detectable in the nucellus of infected apple flower buds.…”

Section: Discussionmentioning

confidence: 99%

Seed and pollen transmission of Apple latent spherical virus in apple

et al. 2010

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To examine whether Apple latent spherical virus (ALSV) has spread among apple trees in an orchard, we surveyed 21 apple trees surrounding two ALSV-infected trees for virus infection using a double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA). None of the 21 trees were infected, indicating that ALSV has not spread from the infected trees to the neighboring apple trees since it was first detected in 1984. We analyzed seed embryos and seedlings derived from infected trees and detected ALSV in 10 of 223 seed embryos (4.5%) and 10 of 227 seedlings (4.4%). From these results, we conclude that ALSV is seed-transmitted at a rate of ca. 4.5% in apple. We also analyzed seed embryos and seedlings from uninfected apple trees that were hand-pollinated with pollen from infected trees. We detected ALSV in only 1 of 260 seed embryos and in none of the 227 apple seedlings. This result indicated that the seed transmission rate via infected pollen is only 0-0.38%. In situ hybridization analysis of ALSVinfected apple flower buds showed that ALSV was present inside almost all pollen grains and in all ovary and ovule tissues, including the embryo sac and inner integument.

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Section: Discussionmentioning

confidence: 99%

Seed and pollen transmission of Apple latent spherical virus in apple

et al. 2010

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“…It has been demonstrated also that seed transmission is related to the ability of the virus to infect flower organs, especially the meristematic reproductive tissue, during early developmental stages [34]. Similarly, Amari et al [35] have demonstrated that Prunus necrotic ringspot virus (PNRSV) invades apricot reproductive organs, including the pollen mother cell stage, early in development, and after that PNRSV infects all parts of the seed, including embryo, endosperm and testa, shedding light on the vertical transmission of PNRSV from gametes to seedlings [1]. Also the wide natural incidence of the nepovirus Cherry leaf roll virus (CLRV) is believed to have occurred mainly by seed transmission, in fact vertical transmission of CLRV by seed and pollen has been reported to occur naturally by embryo invasion in different works [36][37][38].…”

Section: Resultsmentioning

confidence: 99%

“…In conclusion, much research has focused on the routes by which the seeds become infected and on the progress of viral infection in reproductive organs during their development up until the seedling stage [1,7,24]. Few researchers have addressed the problem of the correlation between breeding techniques and seed transmission of viruses.…”

Section: Introductionmentioning

confidence: 99%

“…The direct and indirect embryonal transmission affects viruses that extensively invade parenchymal tissues and are transmitted by mechanical inoculation. However, little is known about how viruses can move from sporophytic to gametophytic tissues and back again into sporophytic tissues [1].…”

Section: Introductionmentioning

confidence: 99%

“…Seed transmission plays an important role in the dissemination of some viruses, especially as a result of breeding programs [1]. In fact, seed and, to a lesser extent, pollen are the natural pathway through which some plant viruses are transmitted to the progeny of hosts (vertical transmission) and spread to the environment [2].…”

Section: Introductionmentioning

confidence: 99%

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Study of main grapevine viruses transmission in breeding programs

Gasparro¹,

Caputo²,

Forleo³

et al. 2016

BIO Web Conf.

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Abstract.A number of studies have established that vertical transmission of viruses is an important phenomenon affecting a wide range of viruses, especially as a result of breeding programs. Nevertheless, the presence of virus in a seed, does not always lead to seedling infection. In grapevine, seed transmission has been reported for many nepoviruses, but little is known about the other virus associated diseases such as Leafroll, Rugose wood complex and Fleck diseases, that are considered by Italian legislation on certification of grapevine propagation material, together with infectious degeneration. Thus, the aim of this study is monitoring the virological condition of seedlings obtained by crosses between infected parentals, analyzing the sanitary status of seedlings after the transfer in experimental fields. In this study, 50 progeny plants from three different traditionally crossbreed seeded and seedless table grape cultivars were investigated for the detection of nine grapevine viruses. It was observed that the viral state of parentals was quite compromised, while viruses were not detected in any of the 150 progeny plants, demonstrating that the main grapevine viruses are at low risk for seed transmission. These results could make it possible to reduce the precautions to obtain propagation material free from infectious diseases and also suggest to consider reproduction as a new procedure of sanitation in breeding programs.

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Bromoviridae and Allies

Scott

2011

Encyclopedia of Life Sciences

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The family Bromoviridae consists of six genera: Alfamovirus , Anulavirus , Bromovirus , Cucumovirus , Ilarvirus and Oleavirus . All viruses in these genera possess tripartite genomes of single‐stranded, linear, positive‐sense ribonucleic acid (RNA), in which expression of the downstream codon of the RNA3 is via a subgenomic messenger RNA (mRNA) (RNA4). Raspberry bushy dwarf virus (RBDV) was the sole member of the genus Idaeovirus for many years and shares features in common with members of the genus Ilarvirus and Alfamovirus , but possesses a bipartite genome and is not currently assigned to a family. Members of the genera Alfamovirus , Bromovirus and Cucumovirus have been used extensively as model systems for research on molecular aspects of plant virology. Key Concepts: Although all members of the Bromoviridae have similar particle morphologies (quasi‐spherical and bacilliform) and possess tripartite genomes, a range of different strategies is used to express the products of the different genomes. The phenomenon of genome activation by the coat protein was unique to the ilarviruses and Alfalfa mosaic virus but has recently been demonstrated for RBDV. The natural host ranges of Cucumber mosaic virus and Alfalfa mosaic virus are extensive whereas many other viruses described in this article have an extremely limited natural host range. Complete genomic nucleotide sequences are available from GenBank for the majority of viruses referred to in this article.

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Vertical transmission of Prunus necrotic ringspot virus: hitch-hiking from gametes to seedling

Cited by 51 publications

References 44 publications

Seed and pollen transmission of Apple latent spherical virus in apple

Seed and pollen transmission of Apple latent spherical virus in apple

Study of main grapevine viruses transmission in breeding programs

Bromoviridae and Allies

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