Influence of the solvation process on solute adsorption in reversed phase liquid chromatography

Comparison of the Retention of Aliphatic Hydrocarbons with Polar Groups in RP-HPLC Systems with Different Modifiers of the Binary Eluent

“…It should be noticed that alcohols, donors and acceptors of protons, can strongly interact with the methanol contained in the stationary phase. Also formations of solvated species in the stationary phase region with self-associated methanol molecules and/or methanol/water complexes could lead to enhancement of their retention [26]. A similar effect was observed in our earlier studies of aromatic alcohols [6, 10].…”

Preparation and evaluation of hydrophilic C18 monolithic sorbents for enhanced polar compound retention in liquid chromatography and solid phase extraction

“…ACN), hydrogen bonding was, therefore, not the dominant interaction under the elution conditions. Replacing methanol with a stronger eluent as organic modifier results in a fundamental change in the shape of the adsorption isotherms of phenol [33]. Satisfactory results were achieved using ACN as eluting solvent, with recoveries ranging from 68.0% to 105.0%.…”