Influence of the Spleen on the Blood Distribution of the Colony-Forming Cells (CFU-C) in Man

Morra, L; Ponassi, A; Bruzzi, Paolo; Parodi, Gíovanní; Colella, Giuseppe; Sacchetti, C.

doi:10.1159/000207102

Cited by 4 publications

(3 citation statements)

References 6 publications

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“…In cirrhotic patients the con centration of blood CFU-GM was slightly reduced, but the difference to normal sub jects was not statistically significant. A slight decrease of blood CFU-GM, due to increased margination within the spleen, had been previously reported; CFU-GM could be removed from the spleen to blood with epinephrine [6]. The nonsigni ficant decrease of blood CFU-GM alone cannot explain the greatly decreased gran ulocytic growth we found in liquid cul tures of cells from cirrhotic patients.…”

Section: Discussionsupporting

confidence: 63%

“…It may be the expression of a more generally increased turnover of the monocyte-mac rophagic system in these patients that re quires further investigation. Our data seem to indicate that both granulocyte trapping within the spleen, associated or not with distruction [1,6], and reduced granulocyte production may be present in cirrhotic patients. The latter does not ap pear to be due to decreased concentration of CFU-GM, but possibly to increased committment of CFU-GM towards the monocyte-macrophagic line or to mecha nisms operating directly on the proliferat ing granulocytic pool.…”

Section: Discussionmentioning

confidence: 76%

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Blood Granulocyte-Macrophage Progenitor Cell Concentrations and Differentiation in vitro in Patients with Hepatic Cirrhosis

Ponassi¹,

Morra²,

Colella³

et al. 1984

Acta Haematol

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Blood granulocyte-macrophage progenitor cells (CFU-GM) and myeloid differentiation were studied in 16 patients with hepatic cirrhosis by culturing blood mononuclear cells in semisolid and in liquid medium. The numbers of CFU-GM from normal and cirrhotic subjects were not significantly different, even when increased numbers of monocyte-macrophagic colonies and decreased numbers of granulocytic colonies were observed in cirrhotic patients. Significantly reduced granulocytic growth and increased monocyte-macrophagic cell growth were found in liquid culture of cirrhotic patients. These data seem to indicate that in hepatic cirrhosis, besides granulocyte sequestration within the spleen, there is a disorder of granulocytic versus monocyte-macrophagic differentiation.

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Section: Discussionsupporting

confidence: 63%

Section: Discussionmentioning

confidence: 76%

Blood Granulocyte-Macrophage Progenitor Cell Concentrations and Differentiation in vitro in Patients with Hepatic Cirrhosis

Ponassi¹,

Morra²,

Colella³

et al. 1984

Acta Haematol

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“…Golde reported inhibition of murine in vitro colony-forming cells (CFU-C)' by dexamethasone (3) and Zalman et al (4) confirmed this finding, demonstrating an 18% decrease in the number of CFU-C when 6 X 1o-8 M dexamethasone was incorporated into the culture. Finally, in a study of the effect of hydrocortisone on human CFU-C, Morra et al (5) found that 1 mg of hydrocortisone added to in vitro cultures decreased the growth of CFU-C by -24% (5). Using the Luxol Fast Blue stain, it has been shown that 50% of the circulating CFU-C (6) and 10-40% of the bone marrow CFU-C are eosinophilic, with the remainder being neutrophil/macrophage precursors (7,8).…”

Section: Introductionmentioning

confidence: 99%

Differential effect of hydrocortisone on eosinophil and neutrophil proliferation.

Bjornson¹,

Harvey²,

Rose³

1985

J. Clin. Invest.

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Glucocorticosteroid therapy results in an increase in the number of circulating neutrophils and a decrease in the number of eosinophils. Utilizing the double layer soft agar technique, we examined the effect of physiologic to pharmacologic concentrations of hydrocortisone on the proliferation of human neutrophil progenitors and eosinophil progenitors from peripheral blood and bone marrow. When peripheral blood cultures were studied, eosinophil proliferation was inhibited in a dose-responsive fashion with 10"-40-M hydrocortisone succinate, and comprised 49±4% of the colonies in control cultures and only 4±1% (P < 0.01) at pharmacologic levels of hydrocortisone (10-5 M). The number of neutrophil colonies, on the other hand, increased by 31% when 10-5 M hydrocortisone was added to cultures. In order for corticosteroids to exert this effect, it was necessary to add them within 24 h of the initiation of culture. The effect of hydrocortisone on granulocyte proliferation could not be blocked by progesterone, a structurally analogous steroid. To determine whether hydrocortisone was acting directly on the progenitor cell or via an effector cell, its effect on modulating cell populations and stimulating-factor production was studied. Removal of Erosetting cells and/or adherent cells did not affect the inhibition of eosinophil colony growth or the enhancement of neutrophil colony growth. Furthermore, addition of the potent inhibitor of T cell function, cyclosporin A, failed to affect eosinophil colony frequency, suggesting that inhibition of T cell function was an unlikely explanation for the observed hydrocortisone effect. Leukocyte conditioned media (LCM), derived from peripheral blood mononuclear cells incubated with hydrocortisone, was devoid of both neutrophil and eosinophil colony-stimulating activity, whereas a control LCM stimulated both neutrophil and eosinophil proliferation. The data suggest that the observed hydrocortisone effect on granulocyte colony formation is unlikely to be mediated by an intermediary, and that hydrocortisone acts directly on progenitor cells.

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Chronobiology of Circulating Blood Cells and Platelets

Haus¹

1992

Biologic Rhythms in Clinical and Laboratory Medicine

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Influence of the Spleen on the Blood Distribution of the Colony-Forming Cells (CFU-C) in Man

Cited by 4 publications

References 6 publications

Blood Granulocyte-Macrophage Progenitor Cell Concentrations and Differentiation in vitro in Patients with Hepatic Cirrhosis

Blood Granulocyte-Macrophage Progenitor Cell Concentrations and Differentiation in vitro in Patients with Hepatic Cirrhosis

Differential effect of hydrocortisone on eosinophil and neutrophil proliferation.

Chronobiology of Circulating Blood Cells and Platelets

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