Influence of Thidiazuron in Direct Shoot Regeneration From Segments of in Vitro Leaves, and Axillary and Apical Buds of Olive (Olea Europaea)

Bahrami, Mohammad Kazem; Azar, Alireza Motallebi; Dadpour, Mohammad Reza

doi:10.17660/actahortic.2010.884.46

Cited by 3 publications

(2 citation statements)

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“…Although olive is generally difficult to manipulate in vitro , it has been possible to micropropagate selected olive cultivars through nodal segmentation of elongated shoots (Rugini, 1984; Roussos and Pontikis, 2002; Lambardi et al, 2013). In few cases, buds (Bahrami et al, 2010) or plants (Mencuccini and Rugini, 1993) have been obtained through adventitious organogenesis from petiole and leaf sections derived from in vitro grown shoots of adult origin. However, the most widely used method for adventitious regeneration in both cultivated and wild olive is somatic embryogenesis, although, in this case, most investigations have been carried out with juvenile material, i.e., either immature zygotic embryos (Rugini, 1988) or radicle and cotyledon segments from mature embryos (Orinos and Mitrakos, 1991; Mitrakos et al, 1992; Cerezo et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Plant Regeneration via Somatic Embryogenesis in Mature Wild Olive Genotypes Resistant to the Defoliating Pathotype of Verticillium dahliae

et al. 2019

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Regeneration capacity, via somatic embryogenesis, of four wild olive genotypes differing in their response to defoliating Verticillium dahliae (resistant genotypes StopVert, OutVert, Ac-18 and the susceptible one, Ac-15) has been evaluated. To induce somatic embryogenesis, methodologies previously used in wild or cultivated olive were used. Results revealed the importance of genotype, explant type, and hormonal balance in the induction process. Use of apical buds obtained from micropropagated shoots following a methodology used in cultivated olive (4 days induction in liquid 1/2 MS medium supplemented with 30 µM TDZ–0.54 µM NAA, followed by 8 weeks in basal 1/2 MS medium) was adequate to obtain somatic embryos in two genotypes, StopVert and Ac-18, with a 5.0 and 2.5% induction rates, respectively; however, no embryogenic response was observed in the other two genotypes. Embryogenic cultures were transferred to basal ECO medium supplemented with 0.5 µM 2iP, 0.44 µM BA, and 0.25 µM indole-3-butyric acid (IBA) for further proliferation. Somatic embryos from StopVert were maturated and germinated achieving a 35.4% conversion rate. An analysis of genetic stability on StopVert, using Simple Sequence Repeats (SSRs) and Random Amplified Polymorphic DNA (RAPDs) markers, was carried out in embryogenic callus, plants regenerated from this callus and two controls, micropropagated shoots used as explant source, and the original mother plant. Polymorphism was only observed in the banding pattern generated by RAPDs in 1 of the 10 callus samples evaluated, resulting in a variation rate of 0.07%. This is the first time in which plants have been regenerated via somatic embryogenesis in wild olive.

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Section: Introductionmentioning

confidence: 99%

Plant Regeneration via Somatic Embryogenesis in Mature Wild Olive Genotypes Resistant to the Defoliating Pathotype of Verticillium dahliae

et al. 2019

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“…Although several improvements have been achieved in the last years, the in vitro culture of olive is currently not a technique used in commercial laboratories. Such failure is mainly due to the different behavior of the various cultivars, which requires setting up a specific protocol for each variety (Bahrami et al, 2010;Lambardi and Rugini, 2003;Rostami and Shahsavar, 2012), and the use of zeatin, which is very expensive (Mendozade Gyves et al, 2008). To solve or reduce these problems, efforts have been made to identify alternative cytokinins or compounds/ protocols able to improve the regeneration efficiency of the explants to reduce the unit cost of regenerated shoots (Ali et al, 2009;Mendoza-de Gyves et al, 2008).…”

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confidence: 99%

Neem Oil Used as a “Complex Mixture” to Improve In Vitro Shoot Proliferation in Olive

Micheli

Silva

Farinelli

et al. 2018

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Shoots of the olive cultivar Moraiolo were previously cultured in aseptic conditions on Olive Medium (OM), with the addition of 4 mg·L−1 of zeatin, 30 g·L−1 of sucrose, and 7 g·L−1 of agar. Then, 1-cm long uninodal explants with two leaves and two axillary buds were excised from the proliferated masses and placed on the same proliferation medium enriched with four concentrations of neem oil (0—control, 0.1, 0.5, and 1.0 mL·L−1), added before sterilization. The addition of 0.1 mL·L−1 of neem oil to the medium gave an improvement in shoot regeneration. More vigorous shoots (longer proliferated shoots) were obtained along with a higher number of nodes (multiplication rate). Overall, there was a significant increase in the total fresh and dry proliferated weights. To our knowledge, this is the first report showing a strong and beneficial effect of neem oil, used as a “complex mixture,” on in vitro plant regeneration.

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Use of TDZ for Micropropagation of Some Mediterranean Crop Species

Mazri

Belkoura

Meziani

2018

Thidiazuron: From Urea Derivative to Plant Growth Regulator

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Influence of Thidiazuron in Direct Shoot Regeneration From Segments of in Vitro Leaves, and Axillary and Apical Buds of Olive (Olea Europaea)

Cited by 3 publications

References 0 publications

Plant Regeneration via Somatic Embryogenesis in Mature Wild Olive Genotypes Resistant to the Defoliating Pathotype of Verticillium dahliae

Plant Regeneration via Somatic Embryogenesis in Mature Wild Olive Genotypes Resistant to the Defoliating Pathotype of Verticillium dahliae

Neem Oil Used as a “Complex Mixture” to Improve In Vitro Shoot Proliferation in Olive

Use of TDZ for Micropropagation of Some Mediterranean Crop Species

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