Influence of thidiazuron (TDZ) pretreatment of shoot tips on shoot multiplication and ex vitro acclimatization of Harpagophytum procumbens

Abstract

The effects of thidiazuron (TDZ) pretreatment of shoot tips on Harpagophytum procumbens shoot proliferation and successive stages of micropropagation, i.e. rooting of regenerated shoots and acclimatization of plantlets to ex vitro conditions, were described in the present study. The best response in terms of shoot proliferation (about seven shoots/explant) and shoot length (3.2 ± 0.4 cm) was obtained when explants pretreated with 25 lmol L -1 TDZ for 6 h were cultured on Schenk and Hildebrandt medium containing indole-3-acetic acid (IAA) (0.57 lmol L -1 ) and 6-benzylaminopurine (BAP) (8 lmol L -1 ). Under these conditions, a 330 % increase in shoot multiplication over TDZ non-pretreatment culture was achieved and TDZ pretreatment shoots were longer compared to those in control culture (2.6 ± 0.3 cm). The TDZ pretreatment did not affect the percentage of rooted shoots, length of roots and number of roots formed per shoot. The rooted plantlets were transplanted from in vitro to pots with soil and grown during 1 year in the greenhouse. The hardening process was difficult and time-consuming. We found that the plants developed from the TDZ pretreated culture were superior to plants from non-pretreated culture in terms of survival rate and morphological features, such as shoot length, leaf size, flowering and earlier root tuberisation. Random amplified polymorphic DNA and inter-simple sequence repeat analyses of pretreatment with TDZ plants showed genetic similarity to non-pretreatment plants. We conclude that applying the strategy of initial explant pretreatment with TDZ may be valuable for the improvement in H. procumbens in vitro propagation.

Influence of Plant Growth Regulators on In Vitro Shoot Multiplication and Plantlet Formation in Cassia angustifolia Vahl

“…At the same concentration, BA induced 5.3 ± 0.26 shoots in 80% cultures, while Kin produced 3.6 ± 0.35 shoots with 71% regeneration frequency ( Table 1). The stimulating effect of TDZ on multiple shoot formation has been reported earlier for different medicinal plant species (Sivenesan et al 2011;Guo et al 2011;Grabkowska et al 2014;Singh and Dwivedi 2014). All regenerated shoots were free from any basal callusing.…”

In vitro propagation and preliminary results of Agrobacterium -mediated genetic transformation of Cordyline fruticosa

“…TDZ-induced shoot organogenesis from different explants of many recalcitrant species as well as from woody plants has been reported earlier (Liu et al, 2004;Dewir et al, 2010Dewir et al, , 2011El-Mahrouk et al, 2010;Shaik et al, 2010). However, TDZ proved effective for in vitro propagation of many plant species (Huetteman and Preece, 1993;Baskaran and Jayabalan, 2009;Parveen and Shahzad, 2011;Grąbkowska et al, 2014) but not effective for others (Dewir et al, 2006b;Mitras et al, 2009) i.e. it is species specific.…”

Optimasi Regenerasi Padi Indica melalui Jalur Organogenesis

“…Untuk tanaman berkayu kombinasi BA dan TDZ banyak digunakan dan memberikan hasil yang baik dalam memproduksi tunas in vitro. Grabkowska et al (2014) melaporkan bahwa TDZ dapat menginduksi pembentukan tunas adventif, proliferasi tunas adventif pada tanaman Harpagophytum procumbens.…”