Abstract. Khumaida N, Syukur M, Bintang M, Nurcholis W. 2019. Phenolic and flavonoid content in ethanol extract and agro-morphological diversity of Curcuma aeruginosa accessions growing in West Java, Indonesia. Biodiversitas 20: 656-663. Curcuma aeruginosa is a rhizomatous medicinal plant with beneficial pharmacological activities. The aim of this work was to analyze the agro-morphological, extract yield, and phenolic content of ten C. aeruginosa accessions which were collected from different locations in Indonesia. Cultivation was carried out in the open field in West Java of Indonesia using a completely randomized design. Qualitative and quantitative parameters were used to investigate agro-morphological traits. Total phenolic and total flavonoids contents were determined in ethanol extracts of samples. The plants were phenotypically diverse, in which there were significant variations among the ten C. aeruginosa accessions in number of leaves, plant height, number of shoots, fresh weight of rhizome, and dry weight of rhizome characters. Variability in the total phenolic and total flavonoid contents ranged from 29.08-46.92 mg GAE/g, and 21.31-33.81 mg QE/g, respectively. Six accessions had high phenolic content and extract yield. Therefore, these accessions could be utilized for commercial scale and also showed a high potency for medicinal plant breeding programs.
The use of somatic embryo for in vitro selection program is very useful since the selected traits will be inherited in the progeny. This study was aimed to obtain somaclonal variants for drought tolerances of soybean genotypes from in vitro selection using polyethylene glycol (PEG). The experiments were set up in two factors with completely randomize design and each treatment was replicated ten times. Four soybean genotypes (Tanggamus, Yellow Biloxi, were used in this study as first factor experiment. In vitro selection was in embryogenic callus level using media containing PEG (0, 5, 10, 15, and 20%) (Tanggamus, Yellow Biloxi, digunakan dalam percobaan ini sebagai faktor perlakuan pertama. Seleksi ini vitro dilakukan pada tahap kalus embriogenik seluruh genotipe yang dicoba menggunakan media dengan penambahan PEG (0, 5, 10, 15, and 20%
ABSTRAK
Penggunaan embrio somatik pada program seleksi in vitro sangat dibutuhkan untuk mendapatkan karakter yang diinginkan serta dapat diturunkan pada generasi berikutnya. Tujuan dari penelitian ini adalah untuk mendapatkan varian somaklon tanaman kedelai yang toleran kekeringan melalui seleksi in vitro dengan agen seleksi PEG (polyethylene glycol). Penelitian ini menggunakan rancangan percobaan acak kelompok dengan dua faktor perlakuan dan masing-masing perlakuan diulang 10 kali. Empat genotipe kedelai
Cassava (Manihot esculenta Crantz., Euphorbiaceae) is an important dietary carbohydrate source for approximately 800 million people in the tropics. Cassava breeding through conventional approaches are hampered with some limitations which resulted in a low number of superior varieties. The objective of this research was to generate several mutant lines with higher yield and starch content. According to field studies it was found that several cassava mutant lines have higher yield (root fresh weight >10-20 kg plant -1 ). Two mutant lines has a high starch content (>39%). However, diversity on some variables are still found in M1V2 generation of cassava.
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