Influence of three BALB/c substrain backgrounds on the skin tumor induction efficacy to DMBA and TPA cotreatment

Kang, Mi Ju; Gong, Jeong Eun; Kim, Ji Eun; Choi, Hak Jong; Bae, Su Ji; Choi, Yun Ju; Lee, Su Jin; Seo, Min–Soo; Kim, Kil Soo; Jung, Young-Suk; Cho, Joon-Yong; Lim, Yong; Hwang, Dae Youn

doi:10.1186/s42826-020-00063-z

Cited by 5 publications

(5 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, other tumor phenotypes, including histopathology, apoptotic protein levels and tumor-related protein levels, were similarly induced. These patterns were likewise observed in the anti-tumor response to cisplatin treatment [ 5 ]. In the current study, we examined for variations in the anti-tumor response of cisplatin in three BALB/c substrains bearing CT26 syngeneic tumors.…”

Section: Discussionmentioning

confidence: 78%

“…Furthermore, the BALB/cAnNCrl substrain was established from BALB/cJ and BALB/cByJ mice in the period 1950–1970 [ 4 ]. Recently, a new substrain (BALB/cKorl mice) was isolated from BALB/cJ, at the Department of Laboratory Animal Resources of the National Institute of Food and Drug Safety Evaluation (NIFDS, Chungju, Korea) [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

“…A significant difference was observed in the sensitivity for dexamethasone-induced osteonecrosis when comparing the BALB/cJ and BALB/cAnNHsd substrains [ 9 ]. The differences in skin tumor induction efficacy to DMBA and TPA cotreatment were also analyzed in three BALB/c substrains, including BALB/cKorl [ 5 ]. However, no study has examined the outcomes in the anti-tumor response to cisplatin in BALB/c substrains with syngeneic tumors.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Comparison of cisplatin-induced anti-tumor response in CT26 syngeneic tumors of three BALB/c substrains

et al. 2021

Self Cite

View full text Add to dashboard Cite

Background To determine whether the background of BALB/c substrains affects the response to anti-tumor drugs, we measured for alterations in tumor growth, histopathological structure of the tumor, and expressions of tumor-related proteins in three BALB/c substrains derived from different sources (BALB/cKorl, BALB/cA and BALB/cB), after exposure to varying concentrations of cisplatin (0.1, 1 and 5 mg/kg). Results Cisplatin treatment induced similar responses for body and organ weights, serum analyzing factors, and blood analyzing factors in all BALB/c substrains with CT26 syngeneic tumor. Few differences were detected in the volume and histopathological structure of the CT26 tumor. Growth inhibition of CT26 tumors after exposure to cisplatin was greater in the BALB/cB substrain than BALB/cKorl and BALB/cA substrains, and a similar pattern was observed in the histopathological structure of tumors. However, the expression levels of other tumor-related factors, including Ki67, p27, p53, Bcl-2-associated X protein (Bax), B-cell lymphoma 2 (Bcl-2), caspase-3 (Cas-3), matrix metallopeptidase 2 (MMP2) and vascular endothelial growth factor (VEGF) proteins, were constantly maintained in the tumors of all three substrains after cisplatin treatment. A similar decrease pattern was observed for the expressions of inflammatory cytokines, including interleukin (IL)-1β, IL-6 and IL-10, in the CT26 tumors of the three BALB/c substrains. Conclusions Taken together, results of the present study indicate that the genetic background of the three BALB/c substrains has no major effect on the therapeutic responsiveness of cisplatin, except growth and histopathology of the CT26 syngeneic tumor.

show abstract

Section: Discussionmentioning

confidence: 78%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Comparison of cisplatin-induced anti-tumor response in CT26 syngeneic tumors of three BALB/c substrains

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…Whole blood was collected directly from the heart, placed in heparinized tubes and allowed to clot for 30 min at room temperature. Thereafter, the blood was centrifuged at 3000 rpm for 10 min at 4 °C to obtain serum, which was stored at −80 °C for subsequent analysis, as previously described [ 27 ]. After perfusion, the organs were immediately excised and weighed.…”

Section: Methodsmentioning

confidence: 99%

The Protective Effect of Topical Spermidine on Dry Eye Disease with Retinal Damage Induced by Diesel Particulate Matter2.5

et al. 2021

View full text Add to dashboard Cite

Air pollutants, especially ambient fine particulate matter2.5, may contribute to various ocular surface disorders, including dry eye disease, keratitis and conjunctivitis. A natural polyamine spermidine has a protective effect on the retina and optic nerve; however, no study has been conducted on the application of spermidine in particulate matter2.5-induced dry eye disease. In the present study, we investigated the effect of spermidine eye drops in topically exposed particulate matter2.5-induced dry eye models of Sprague-Dawley rats, by hematological, biochemical and histological evaluation. Spermidine eye drops attenuated the particulate matter2.5 exposure-induced reduction of tear secretion and corneal epithelial damage. Furthermore, spermidine protected against conjunctival goblet cell loss and retinal ganglion cell loss induced by particulate matter2.5. Additionally, spermidine markedly prevented particulate matter2.5-induced infiltration of cluster of differentiation3+ and cluster of differentiation4+ T lymphocytes and F4/80+ macrophages on lacrimal gland. Moreover, over expression of pro-inflammatory cytokines, including tumor necrosis factor-α, interleukin-6 and interleukin-17 in the lacrimal gland and cornea. Meanwhile, the levels of serum total cholesterol and low-density lipoprotein cholesterol were markedly increased by topical exposure to particulate matter2.5, but this change in the lipid profile was decreased by spermidine. Taken together, spermidine may have protective effects against particulate matter2.5-induced dry eye symptoms via stabilization of the tear film and suppression of inflammation and may in part contribute to improving retinal function and lipid metabolism disorder.

show abstract

“…The rats in all the groups were euthanized on day 14. After midline thoracotomy, whole blood and serum were prepared as previously described [ 34 ]. The left ventricle was catheterized using a 23-gage butterfly needle (Becton Dickinson, Franklin Lakes, NJ, USA), the femoral artery was incised and perfused with sterile saline at the rate of 10 mL/min for 3 min.…”

Section: Methodsmentioning

confidence: 99%

The Protective Effect of Oral Application of Corni Fructus on the Disorders of the Cornea, Conjunctiva, Lacrimal Gland and Retina by Topical Particulate Matter 2.5

Lee

Kim

et al. 2021

Nutrients

View full text Add to dashboard Cite

Particulate matter 2.5 (PM2.5) may aggravate dry eye disease (DED). Corni Fructus (CF), which is fruit of Cornus officinalis Sieb. et Zucc., has been reported to have various beneficial pharmacological effects, whereas the effect of CF on the eye is still unknown. Therefore, in this study, we investigated the effect of oral administration of water extract of CF (CFW) on the eye, hematology, and biochemistry in a DED model induced by topical exposure to PM2.5. Furthermore, the efficacy of CFW compared with cyclosporine (CsA), an anti-inflammatory agent, and lutein, the posterior eye-protective agent. Sprague-Dawley rats were topically administered 5 mg/mL PM2.5 in both eyes four times daily for 14 days. During the same period, CFW (200 mg/kg and 400 mg/kg) and lutein (4.1 mg/kg) were orally administered once a day. All eyes of rats in the 0.05% cyclosporine A (CsA)-treated group were topically exposed to 20 μL of CsA, twice daily for 14 days. Oral administration of CFW attenuated the PM2.5-induced reduction of tear secretion and corneal epithelial damage. In addition, CFW protected against goblet cell loss in conjunctiva and overexpression of inflammatory factors in the lacrimal gland following topical exposure to PM2.5. Furthermore, CFW markedly prevented PM2.5-induced ganglion cell loss and recovered the thickness of inner plexiform layer. Meanwhile, CFW treatment decreased the levels of total cholesterol and low-density lipoprotein cholesterol in serum induced by PM2.5. Importantly, the efficacy of CFW was superior or similar to that of CsA and lutein. Taken together, oral administration of CFW may have protective effects against PM2.5-induced DED symptoms via stabilization of the tear film and suppression of inflammation. Furthermore, CFW may in part contribute to improving retinal function and lipid metabolism disorder.

show abstract

Influence of three BALB/c substrain backgrounds on the skin tumor induction efficacy to DMBA and TPA cotreatment

Cited by 5 publications

References 26 publications

Comparison of cisplatin-induced anti-tumor response in CT26 syngeneic tumors of three BALB/c substrains

Comparison of cisplatin-induced anti-tumor response in CT26 syngeneic tumors of three BALB/c substrains

The Protective Effect of Topical Spermidine on Dry Eye Disease with Retinal Damage Induced by Diesel Particulate Matter2.5

The Protective Effect of Oral Application of Corni Fructus on the Disorders of the Cornea, Conjunctiva, Lacrimal Gland and Retina by Topical Particulate Matter 2.5

Contact Info

Product

Resources

About