Influence of tissue preparation on the high-frequency acoustic properties of normal kidney tissue

Does decalcification alter the tissue sound speed of rabbit supraspinatus tendon insertion? In vitro measurement using scanning acoustic microscopy

“…In 1996, Sasaki et al evaluated the effect of formalin fixation and paraffin embedding using normal kidney tissue. In their results, the relative relationships of the high-frequency acoustic properties was well preserved after these preparation process [10].…”

Non-mineralized fibrocartilage shows the lowest elastic modulus in the rabbit supraspinatus tendon insertion: Measurement with scanning acoustic microscopy

“…However, it has been already confirmed that such methods do not change significantly the tissue acoustic properties. 23 Thus, we believed that the relative relationships of the acoustic properties in the specimen would be preserved in the SAM measurement. Third, the Poisson's ratio and the density of the tissue should be measured at the microscopic level to determine the exact Young's modulus of the tissue.…”

Pulmonary imaging with a scanning acoustic microscope discriminates speed-of-sound and shows structural characteristics of disease

“…Sasaki et al 13 reported that no significantly variable properties of the normal kidney were found between these different preparations. Our preliminary data on lung tissues also showed no significant difference in sound speed between formalin-fixed and fresh frozen tissues.…”