Influence of titanium on in vitro fibroblast–<i>Porphyromonas gingivalis</i> interaction in peri‐implantitis

Irshad, Muhammad; Scheres, Nina; Crielaard, Wim; Loos, Bruno G.; Wismeijer, Daniël; Laine, Marja L.

doi:10.1111/jcpe.12136

Cited by 52 publications

(52 citation statements)

References 53 publications

(60 reference statements)

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“…Although in the present study the cells were exposed to the same concentration (50 ng/mL) of micro and nanoparticles of titanium, the highest gene expression for all cytokines was found in the nanoparticles group. As described by previous studies (16,17), pro-inflammatory effect can be related to the titanium particle size. Probably because titanium nanoparticles are more efficiently absorbed by macrophages, inducing an intense effect on gene expression (18).…”

Section: Pro-inflammatory Analysis Of Macrophagesmentioning

confidence: 95%

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Pro-inflammatory Analysis of Macrophages in Contact with Titanium Particles and Porphyromonas gingivalis

et al. 2017

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During insertion of titanium dental implants, particles may shear from the implant to the periimplant region causing osteolysis, and their association with bacteria can exacerbate the inflammatory reaction. However, the association of a high invasive bacterium from the oral cavity, Porphyromonas gingivalis (Pg), and titanium particles remains unknown. This study evaluated pro-inflammatory reaction of human macrophages in contact with micro and nanoparticles of titanium associated with Porphyromonas gingivalis lipopolysaccharide (PgLPS). THP-1 cell were used and treated for 12, 24 and 48 h following 6 groups: Control(C), PgLPS (L); Microparticles (M); Nanoparticles (N); PgLPS and microparticles (LM); PgLPS and nanoparticles (LN). The following assays were carried out: i) cell viability using MTS, ii) cell morphology by SEM and iii) expression of tumor necrosis factor alpha (TNF-α), interleukin-1 beta (IL-1β) and interleukin-6 (IL-6) by qRT-PCR and ELISA. For statistics two-way ANOVA followed by Tukey's test was used (p<0.05). After treatment, cells presented similar viability and morphology demonstrating that the treatments were not able to induce cell death. Gene expression was significantly higher for TNF-α and IL1-β after 12 h, and for IL-6 after 24 h in the N and LN groups. Cytokine production over time was an ascending curve for TNF-α with the peak at 48 h and IL1-β and IL-6 had a straight line among the time points, although cells from N group presented a significant production of IL-6 at 48 h. In conclusion, these results suggest that titanium nanoparticles stimulate stronger pro-inflammatory response in macrophages, independent of their association with LPS from P.gingivalis.

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Section: Pro-inflammatory Analysis Of Macrophagesmentioning

confidence: 95%

“…This study investigated the behavior of human macrophages in the presence of titanium micro and nanoparticles associated with P. gingivalis lipopolysaccharide, an important pathogen for periodontal and periimplant disease (16,17).…”

Section: Discussionmentioning

confidence: 99%

Pro-inflammatory Analysis of Macrophages in Contact with Titanium Particles and Porphyromonas gingivalis

et al. 2017

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“…Nevertheless, because several periodontopathogens (such as Aggregatibacter actinomycetemcomitans, Prevotella intermedia, and Porphyromonas gingivalis) associated with the etiology of periodontitis also have been isolated from peri-implant sulci of patients with peri-implantitis, [38][39][40][41] it is arduous to disregard the hypothesis that periimplantitis is more common in patients with a history of periodontitis.…”

Section: Romanos Et Almentioning

confidence: 94%

Peri-implant Diseases

Romanos

Javed

Delgado-Ruíz

et al. 2015

Dental Clinics of North America

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“…Irshad et al showed that peri‐implant granulation tissue fibroblasts, which are exposed to TiO 2 in combination with Porphyromonas gingivalis or separately, can induce a pro‐inflammatory response 35. It has been suggested that Ti ions can elevate the sensitivity of gingival epithelial cells to microorganisms and cause a monocyte infiltration in the gingiva 36.…”

Section: Introductionmentioning

confidence: 99%

Effect of cobalt ions on the interaction between macrophages and titanium

Pettersson

Thorén

et al. 2018

J Biomedical Materials Res

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Inflammation and bone reduction around dental implants are described as peri‐implantitis and can be caused by an inflammatory response against bacterial products and toxins. Titanium (Ti) forms aggregates with serum proteins, which activate and cause release of the cytokine interleukin (IL‐1β) from human macrophages. It was hypothesized that cobalt (Co) ions can interact in the formation of pro‐inflammatory aggregates, formed by titanium. To test this hypothesis, we differentiated THP‐1 cells into macrophages and exposed them to Ti ions alone or in combination with Co ions to investigate if IL‐1β release and cytotoxicity were affected. We also investigated aggregate formation, cell uptake and human biopsies with inductively coupled plasma atomic emission spectroscopy and electron microscopy. Co at a concentration of 100 µM neutralized the IL‐1β release from human macrophages and affected the aggregate formation. The aggregates formed by Ti could be detected in the cytosol of macrophages. In the presence of Co, the Ti‐induced aggregates were located in the cytosol of the cultured macrophages, but outside the lysosomal structures. It is concluded that Co can neutralize the Ti‐induced activation and release of active IL‐1β from human macrophages in vitro. Also, serum proteins are needed for the formation of metal‐protein aggregates in cell medium. Furthermore, the structures of the aggregates as well as the localisation after cellular uptake differ if Co is present in a Ti solution. Phagocytized aggregates with a similar appearance seen in vitro with Ti present, were also visible in a sample from human peri‐implant tissue. © 2018 The Authors Journal of Biomedical Materials Research Part A Published by Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A:2518–2530, 2018.

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Influence of titanium on in vitro fibroblast–Porphyromonas gingivalis interaction in peri‐implantitis

Cited by 52 publications

References 53 publications

Pro-inflammatory Analysis of Macrophages in Contact with Titanium Particles and Porphyromonas gingivalis

Pro-inflammatory Analysis of Macrophages in Contact with Titanium Particles and Porphyromonas gingivalis

Peri-implant Diseases

Effect of cobalt ions on the interaction between macrophages and titanium

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