Influence of Trolox and Alpha-Lipoic Acid on Post-Thawed Pırlak Ram Sperm Parameters, Oxidative Stress and DNA Damage in Non-Breeding Season

Abstract

The present study was conducted to examine the protective roles of trolox and alpha-lipoic on post-thawed Pirlak ram sperm parameters, oxidative stress and DNA damage in non-breeding season. Semen samples from 5 healty Pırlak rams (2-3 years of age) were used in the study. Six ejaculates for each rams were collected and pooled. Ejaculates were split into three aliquots and diluted to a final concentration of 150x10 6 spermatozoa/ml with the base extender containing 1 mM alpha lipoic acid, 1 mM trolox and no additive (control) were cooled to 5 °C and equilibrated for 3 h then frozen in 0.25 ml French straws. Samples were thawed in 37 o C and evaluated in terms of spermatological parameters, DNA damage, oxidative stress. Sperm motility was increased, acrosome rate and DNA damage were decreased significantly (p<0.05) in ALA, head abnormal sperm rate and membrane integrity were increased, acrosome rate and DNA damege were decreased significantly (P < 0.05) in trolox when compared to the control group. Compared with the control group, reductions in spermatozoon MDA and GSH levels were statistically significant (P <0.05) in lipoic acid and trolox groups. Results of this study suggest that alphalipoic acid and trolox improve sperm parmeters, oxidative stress and DNA damage in non-breeding season.

Effects of alpha‐lipoic acid on ram semen cryopreservation and post‐thaw life span

“…Avdatek et al. (2019) reported that 1 mmol L −1 alpha‐lipoic acid better preserved the plasma membrane integrity compared to their control group. These differences might be caused by the techniques used in the dilution and freezing of semen, and the differences in the content of the diluent.…”

“…Our findings show that 0.25 mmol L −1 and 0.50 mmol L −1 alpha-lipoic acid supplementation increased the post-thaw plasma membrane functional integrity rate compared to the control group. Avdatek et al (2019) reported that 1 mmol L −1 alpha-lipoic acid better preserved the plasma membrane integrity compared to their control group. These differences might be caused by the techniques used in the dilution and freezing of semen, and the differences in the content of the diluent.…”

“…In the study, there were no statistical differences found among groups in terms of the defected acrosome rate (p > .05). Avdatek et al (2019) reported that 1 mmol L −1 alpha-lipoic acid group had a positive effect on acrosome integrity. This difference may be related to their analysis method (Hancock's Method) used in the research.…”

“…Avdatek et al. (2019) reported that alpha‐lipoic acid has positive effects on ram semen after cryopreservation. However, according to our research, we did not see a study examining the long‐term effects of alpha‐lipoic acid on spermatozoa.…”

“…It has been reported that alpha-lipoic acid, added to freezing diluents in order to take advantage of antioxidant properties, has different positive effects on spermatological properties of boar (Shen et al, 2016), cattle (Güngör et al, 2016;Büyükleblebici et al, 2015;Gohar et al, 2014) and goat (Ibrahim et al, 2008) sperm. Avdatek et al (2019) reported that alpha-lipoic acid has positive effects on ram semen after cryopreservation. However, according to our research, we did not see a study examining the long-term effects of alpha-lipoic acid on spermatozoa.…”