Influence of Two-Component Signal Transduction Systems of
            <i>Lactobacillus casei</i>
            BL23 on Tolerance to Stress Conditions

Alcántara, Cristina; Revilla-Guarinos, Ainhoa; Zúñiga, Manuel

doi:10.1128/aem.02176-10

Cited by 32 publications

(46 citation statements)

References 27 publications

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“…The involvement of TCSs in stress responses in LAB has been evidenced mainly by phenotypic analyses of TCS-defective mutants (60)(61)(62)(63)(64)(65)(66)(67). Inactivation of homologous TCSs often results in different phenotypes, suggesting that they have different physiological roles.…”

Section: Two-component Systemsmentioning

confidence: 99%

Stress Physiology of Lactic Acid Bacteria

Papadimitriou

Alegría

Bron

et al. 2016

Microbiol Mol Biol Rev

541

404

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SUMMARYLactic acid bacteria (LAB) are important starter, commensal, or pathogenic microorganisms. The stress physiology of LAB has been studied in depth for over 2 decades, fueled mostly by the technological implications of LAB robustness in the food industry. Survival of probiotic LAB in the host and the potential relatedness of LAB virulence to their stress resilience have intensified interest in the field. Thus, a wealth of information concerning stress responses exists today for strains as diverse as starter (e.g.,Lactococcus lactis), probiotic (e.g., severalLactobacillusspp.), and pathogenic (e.g.,EnterococcusandStreptococcusspp.) LAB. Here we present the state of the art for LAB stress behavior. We describe the multitude of stresses that LAB are confronted with, and we present the experimental context used to study the stress responses of LAB, focusing on adaptation, habituation, and cross-protection as well as on self-induced multistress resistance in stationary phase, biofilms, and dormancy. We also consider stress responses at the population and single-cell levels. Subsequently, we concentrate on the stress defense mechanisms that have been reported to date, grouping them according to their direct participation in preserving cell energy, defending macromolecules, and protecting the cell envelope. Stress-induced responses of probiotic LAB and commensal/pathogenic LAB are highlighted separately due to the complexity of the peculiar multistress conditions to which these bacteria are subjected in their hosts. Induction of prophages under environmental stresses is then discussed. Finally, we present systems-based strategies to characterize the “stressome” of LAB and to engineer new food-related and probiotic LAB with improved stress tolerance.

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Section: Two-component Systemsmentioning

confidence: 99%

Stress Physiology of Lactic Acid Bacteria

Papadimitriou

Alegría

Bron

et al. 2016

Microbiol Mol Biol Rev

541

404

View full text Add to dashboard Cite

show abstract

“…The TCS-6 cluster could be not found in L. casei BD-II strain, which is involved in cell envelope stress response, nisin resistance, bile and NaCl response, oxidative stress tolerance, H 2 O 2 stress tolerance of L. casei BL23 strain [10,[35][36][37]. Orphan RR1 was also absent in L. casei BL23 strain.…”

Section: Tcs Proteins Uniquely Present/absent In One or Several Strainsmentioning

confidence: 90%

“…The TCS-1 cluster was predicted to be absent in L. casei W56 strain, which is involved in cell envelope stress tolerance, and the response of the bile, NaCl and antimicrobials of L. casei BL23 (Table 4) [10]. The TCS-3 was also absent in L. casei W56.…”

Section: Tcs Proteins Uniquely Present/absent In One or Several Strainsmentioning

confidence: 99%

“…For instance, TCS-12 is highly conserved across the six L. casei strains. TCS-12 is homologous to the three paralogous TCS of Bacillus subtilis, BceRS, YvcPQ, and YxdJK, involved in the cell envelope stress response against the nisin [10,32]. TCS-12 of L. casei BL23 strain has been found to play a vital role in the growth under a low pH environment [10].…”

Section: Tcs Proteins Common To All the Six L Casei Strainsmentioning

confidence: 99%

“…The TCS MaeKR belonging to the citrate family is essential for the expression of malic enzyme of L. casei strains BL23 and ATCC 334, and MaeKR expression was induced by L-malic acid [9]. The genome sequences of L. casei strains BL23 and ATCC 334 harbor 17 putative TCSs, among which the role of three TCSs involved in bile response, cell envelope stress response, oxidative stress tolerance, and acid tolerance [10]. However, the role of TCSs in L. casei is not still well understood.…”

Section: Introductionmentioning

confidence: 99%

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Comparative Genomic Analysis of Two-Component Signal Transduction Systems in Probiotic Lactobacillus casei

Peng

Chen³

et al. 2014

Indian J Microbiol

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Lactobacillus casei has traditionally been recognized as a probiotic, thus needing to survive the industrial production processes and transit through the gastrointestinal tract before providing benefit to human health. The two-component signal transduction system (TCS) plays important roles in sensing and reacting to environmental changes, which consists of a histidine kinase (HK) and a response regulator (RR). In this study we identified HKs and RRs of six sequenced L. casei strains. Ortholog analysis revealed 15 TCS clusters (HK-RR pairs), one orphan HKs and three orphan RRs, of which 12 TCS clusters were common to all six strains, three were absent in one strain. Further classification of the predicted HKs and RRs revealed interesting aspects of their putative functions. Some TCS clusters are involved with the response under the stress of the bile salts, acid, or oxidative, which contribute to survive the difficult journey through the human gastrointestinal tract. Computational predictions of 15 TCSs were verified by PCR experiments. This genomic level study of TCSs should provide valuable insights into the conservation and divergence of TCS proteins in the L. casei strains.

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Characterization of the binding capacity of mercurial species in Lactobacillus strains

et al. 2017

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BACKGROUND Metal sequestration by bacteria has been proposed as a strategy to counteract metal contamination in foodstuffs. Lactobacilli can interact with metals, although studies with important foodborne metals such as inorganic [Hg(II)] or organic (CH3Hg) mercury are lacking. Lactobacilli were evaluated for their potential to bind these contaminants and the nature of the interaction was assessed by the use of metal competitors, chemical and enzymatical treatments, and mutants affected in the cell wall structure. RESULTS Lactobacillus strains efficiently bound Hg(II) and CH3Hg. Mercury binding by Lactobacillus casei BL23 was independent of cell viability. In BL23, both forms of mercury were cell wall bound. Their interaction was not inhibited by cations and it was resistant to chelating agents and protein digestion. Lactobacillus casei mutants affected in genes involved in the modulation of the negative charge of the cell wall anionic polymer lipoteichoic acid showed increased mercury biosorption. In these mutants, mercury toxicity was enhanced compared to wild‐type bacteria. These data suggest that lipoteichoic acid itself or the physicochemical characteristics that it confers to the cell wall play a major role in mercury complexation. CONCLUSION This is the first example of the biosorption of Hg(II) and CH3Hg in lactobacilli and it represents a first step towards their possible use as agents for diminishing mercury bioaccessibility from food at the gastrointestinal tract. © 2017 Society of Chemical Industry

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Influence of Two-Component Signal Transduction Systems of Lactobacillus casei BL23 on Tolerance to Stress Conditions

Cited by 32 publications

References 27 publications

Stress Physiology of Lactic Acid Bacteria

Stress Physiology of Lactic Acid Bacteria

Comparative Genomic Analysis of Two-Component Signal Transduction Systems in Probiotic Lactobacillus casei

Characterization of the binding capacity of mercurial species in Lactobacillus strains

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