Influence of UV irradiation on penetration of hydrocortisone. In vivo study in hairless rat skin

Lamaud, Evelyne; Schalla, W.

doi:10.1111/j.1365-2133.1984.tb15595.x

Cited by 29 publications

(23 citation statements)

References 7 publications

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“…Exposure of the skin to ultraviolet (UV) radiation induces various harmful effects in that tissue, such as hyperplasia, increases in roughness, wrinkle formation, pigmentation and inflammation (Seidl, 1963;Makki et al, 1979;Bissett et al, 1987;Imokawa et al, 1995), and disruption of the epidermal barrier (Abe and Mayuzumi, 1979;Solomon and Lowe, 1979;Lamaud and Schalla, 1984;McAuliffe and Blank, 1991). Epidermal ceramides (Cer) and their metabolites play critical roles in maintaining cutaneous barrier homeostasis (Elias and Friend, 1975;Gray et al, 1982;Elias, 1983;Landmann, 1986) and decreased levels of Cer in the stratum corneum perturb the barrier (Holleran et al, 1991;Imokawa et al, 1991).…”

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confidence: 98%

Decreased Levels of Covalently Bound Ceramide Are Associated with Ultraviolet B-Induced Perturbation of the Skin Barrier

Takagi

Nakagawa

Kondo

et al. 2004

Journal of Investigative Dermatology

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Although ultraviolet B (UVB) irradiation perturbs the skin barrier, little is known about the mechanism(s) with respect to the metabolism of ceramide (Cer). We examined changes in intercellular lipids in murine stratum corneum following UVB irradiation. A single UVB (75 mJ per cm(2)) irradiation caused a significant increase in transepidermal water loss, which plateaued at day 4. In parallel, covalently bound Cer was significantly decreased with the greatest decrease at days 3-4. In contrast, the levels of other free, non-bound lipids (including Cer or acylceramides) were significantly increased for Cer, or remained unchanged at day 4 compared with non-irradiated controls. RT-PCR analysis demonstrated a significant decrease in mRNA encoding transglutaminase-1 (TGase1). The peak occurred 2-4 d after a single UVB irradiation, a time when covalently bound Cer was significantly downregulated in concert with the disruption of the skin barrier. Furthermore, UVB-induced epidermal hyperplasia occurred to the greatest extent between 2 and 4 d following UVB irradiation. These results suggest that decreases in covalently bound Cer in the stratum corneum are mediated via the downregulation of TGase-1 as well as by the rapid induction of epidermal hyperplasia, which is attributable to the perturbation of the skin barrier induced by UVB irradiation.

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confidence: 98%

Decreased Levels of Covalently Bound Ceramide Are Associated with Ultraviolet B-Induced Perturbation of the Skin Barrier

Takagi

Nakagawa

Kondo

et al. 2004

Journal of Investigative Dermatology

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“…Tropospheric ozone, as a major air pollutant contained in photochemical smog, occurs at a concentration between 0.1 and 0.8 ppm [10]. Whereas ample evidence exists that UVR leads to increased skin permeability for water [11][12][13][14] and other solutes [15,16], such effects have not yet been reported for ozone. While many studies investigating transepidermal water loss (TEWL) in animal models have been carried out in anesthetized animals, little is known about the effect of anesthesia on skin temperature and subsequently TEWL of animals such as hairless mice.…”

Section: Introductionmentioning

confidence: 99%

Impact of Ultraviolet Radiation and Ozone on the Transepidermal Water Loss as a Function of Skin Temperature in Hairless Mice

Thiele

Dreher²,

Maibach³

et al. 2003

Skin Pharmacol Physiol

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Exposure to ultraviolet radiation or ozone leads to skin damage including oxidation of skin biomolecules, as well as to depletion of constitutive antioxidants. The highly organized stratum corneum forming the main barrier against most xenobiotics is particularly susceptible to such damage and possible barrier perturbation may be the consequence. Whereas ample evidence exists for an increased permeability for different solutes including water after exposure to ultraviolet radiation, such an effect has not yet been reported for ozone. This study reports on the effect of such oxidative stressors using the hairless mouse as the skin model and measuring temperature-controlled transepidermal water loss (TEWL) as an indicator for skin barrier integrity. First, a strong dependency of the TEWL on skin temperature was observed, an effect that was clearly more pronounced than that found in man. Given this temperature dependency in untreated animals, we proceeded to determine the effects of both ultraviolet radiation and ozone on TEWL over a relevant physiological skin temperature range. Solar-simulated ultraviolet radiation (0.75–3 minimal erythemal dose) resulted in a delayed and dose-dependent skin barrier disruption over the entire temperature range investigated. Conversely, daily ozone exposure at 2 ppm for 1 week, however, did not significantly alter TEWL up to 72 h after the last exposure. The results demonstrate a differential response of the epidermis to two environmental stressors associated with oxidative damage; they suggest that chronic ozone exposure at relevant environmental levels does not lead to a detectable skin barrier defect, while solar UV exposure was demonstrated to increase epidermal water loss. Furthermore, experimental evidence clearly suggests that future studies applying TEWL measurements in animal models should be performed under carefully controlled skin temperature conditions.

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“…The exposure dose is an important factor in determining the effects of UVB exposure. UV-induced DNA damage and barrier disruption increase linearly with increasing dosage (7). …”

Section: Beneficial Effects Induced By Uvb In the Epidermal Barriermentioning

confidence: 99%

“…UVA predominantly affects the dermis and the DNA, whereas UVB affects the epidermis (1–3). UV irradiation of the skin is known to induce disruption of the epidermal barrier (4–7). However, UVB irradiation has been used for the treatment of atopic dermatitis, a skin disease involving a defective epidermal barrier (8–10).…”

Section: Introductionmentioning

confidence: 99%

Epidermal barrier: Adverse and beneficial changes induced by ultraviolet B irradiation depending on the exposure dose and time (Review)

Permatasari

Zhou

Luo

2013

Experimental and Therapeutic Medicine

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Exposure of the skin to ultraviolet (UV) radiation induces various harmful effects in the tissues, particularly disruption of the epidermal barrier. However, ultraviolet B (UVB) irradiation has been applied in the treatment of atopic dermatitis, a skin disease in which the epidermal barrier is defective. We reviewed the homeostasis of the epidermal barrier and several studies investigating the adverse and beneficial effects caused by different doses of UVB irradiation in the epidermal barrier. It may be concluded that, despite the harmful effects of UVB irradiation on the skin, UVB irradiation is able to exert beneficial effects in the epidermal barrier when administered in suberythemal doses and over a relatively short period of time, with no clinically evident inflammation or barrier disruption. This may be a useful therapeutic strategy for the use of UVB irradiation in the treatment of skin diseases with a disrupted epidermal barrier, such as atopic dermatitis, while reducing or avoiding the side-effects.

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Influence of UV irradiation on penetration of hydrocortisone. In vivo study in hairless rat skin

Abstract: 157DISCUSSION SUMMARY Dr Krueger wondered whether elevated skin temperature or blood flow contributed to the increased corticoid penetration. However, in this model it was thought the thinner horny layer and hyperkeratosis were the major determining factors.

Cited by 29 publications

References 7 publications

Decreased Levels of Covalently Bound Ceramide Are Associated with Ultraviolet B-Induced Perturbation of the Skin Barrier

Decreased Levels of Covalently Bound Ceramide Are Associated with Ultraviolet B-Induced Perturbation of the Skin Barrier

Impact of Ultraviolet Radiation and Ozone on the Transepidermal Water Loss as a Function of Skin Temperature in Hairless Mice

Epidermal barrier: Adverse and beneficial changes induced by ultraviolet B irradiation depending on the exposure dose and time (Review)

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