Influence of ventilation and sucrose on growth and leaf anatomy of micropropagated potato plantlets

Mohamed, M. A.H.; Alsadon, Abdullah A.

doi:10.1016/j.scienta.2009.09.014

Cited by 74 publications

(63 citation statements)

References 23 publications

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“…The results found in this work, with lower pigment accumulation in plantlets cultured in hermetically sealed tubes, especially in culture medium containing NAA, reinforce the hypothesis that the hermetic sealing of the vials could be detrimental to the development of autotrophic plants, reducing the efficiency and protection of the photosynthetic apparatus. Similar results were found by Chanemougasoundharam et al (2004), with Solanum tuberosum, and by Mohamed and Alsadon (2010), for potato plants, for which the ventilation of the culture flasks contributed positively to the chlorophyll b content, not interfering, however, on levels of chlorophyll a.…”

Section: Discussionsupporting

confidence: 82%

“…In acclimatization, several metabolic changes can be activated in an attempt to neutralize the damages caused by hydric stress, including the accumulation of different compatible solutes (Hoekstra et al, 2001;Mohammadkhani and Heidari, 2008). Proline is the primary accumulated metabolite in different stress conditions, which is essential for plant establishment and tolerance (Molinari et al, 2007;Liang et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

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Proline levels, oxidative metabolism and photosynthetic pigments during in vitro growth and acclimatization of Pitcairnia encholirioides L.B. Sm. (Bromeliaceae)

et al. 2016

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This study aimed to evaluate the variation in the levels of proline, oxidative metabolism and photosynthetic pigments in plants of Pitcairnia encholirioides grown in vitro under different conditions and after acclimatization. The analyses were performed after 150 days of in vitro cultivation in MS media supplemented with 10 µM GA 3 or 0.2 µM NAA, sucrose at 15 or 30 g L -1 , in test tubes which allowed gas exchange or in a hermetically sealed system, and 180 days after acclimatization. The in vitro maintenance in hermetically sealed flasks, with GA 3 and 15 g L -1 sucrose had adverse metabolic effects, which was demonstrated by the lower proline and photosynthetic pigments accumulation and by the increase in antioxidant enzymes activities. After acclimatization, differences for proline and photosynthetic pigments were no longer found and the enzymatic activities ranged unevenly. The results suggest that the in vitro cultivation in media with 0.2 µM NAA and 30 g L -1 sucrose, in test tubes capped with closures which allowed gas exchange, is more suitable for micropropagation of P. encholirioides, providing a prolonged maintenance of in vitro cultures and plantlets with superior quality for ex vitro development.Keywords: enzymatic activity, oxidative stress, micropropagation, proline. Niveis de prolina, do metabolismo oxidativo e dos pigmentos fotossintéticos durante o crescimento in vitro e aclimatização de Pitcairnia encholirioides L.B. Sm. (Bromeliaceae) ResumoEste trabalho objetivou avaliar a contribuição da prolina, do metabolismo oxidativo e dos pigmentos fotossintéticos na propagação in vitro e aclimatização de Pitcairnia encholirioides, uma bromélia criticamente ameaçada de extinção. As análises foram realizadas após 150 dias de cultivo in vitro em meio MS suplementado com 10 µM de GA 3 ou 0,2 µM de ANA, 15 ou 30 g L -1 de sacarose, em tubos de ensaio que permitiam trocas gasosas ou em sistema hermeticamente vedado, e também 180 dias após aclimatização. A manutenção in vitro em frascos hermeticamente fechados, com GA 3 e 15 g L -1 de sacarose apresentou efeito metabólico adverso, demonstrado pelo menor acúmulo de prolina e pigmentos fotossintéticos e também pelo aumento das atividades de enzimas antioxidantes. Após aclimatização, as diferenças para prolina e pigmentos fotossintéticos não foram mais encontradas e as atividades enzimáticas variaram de maneira desuniforme. Os resultados sugerem que o cultivo in vitro em meio com 0,2 µM de ANA e 30 g L -1 de sacarose, em tubos fechados com tampas que permitem trocas gasosas, é mais adequado para a micropropagação de P. encholirioides, proporcionando uma manutenção prolongada das culturas in vitro e plântulas com qualidade superior para o desenvolvimento ex vitro.Palavras-chave: atividade enzimática, estresse oxidativo, micropropagação, prolina.

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Section: Discussionsupporting

confidence: 82%

Section: Introductionmentioning

confidence: 99%

Proline levels, oxidative metabolism and photosynthetic pigments during in vitro growth and acclimatization of Pitcairnia encholirioides L.B. Sm. (Bromeliaceae)

et al. 2016

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“…It was also observed that plant grown in tube covered with plug produced large number of roots then cap covered growing plants. Similar report by Mohamed and Alsadon (2009) showed that using ventilated vessels with low sucrose concentration under ambient CO 2 concentration of the growth room could successfully induce photomixotrophic culture resulting in healthy plantlets. Higher leaf dry weight and anatomically well-developed leaves of plantlets were produced in ventilated vessels which facilitate ex vitro acclimation of plantlets.…”

Section: Effect Of Aerophilic and Micro-aerophilic Conditions On Growthsupporting

confidence: 67%

In vitro conservation of exotic potato genotypes through different Incubated temperatures, aerophilic and micro-aerophilic conditions

Ali¹,

Erum²,

Nouroz³

et al. 2016

Int. J. Biodivers. Conserv.

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The present study was carried out to study the in vitro conservation of potato genotypes at different temperatures and aerophilic and micro aerophilic conditions. A total of 31 genotypes were conserved at different incubated temperature ranging from 10 to 25°C. At lower temperature (10°C) plant growth was slowest as compared to plants incubated at high temperature (25°C). The results revealed that aerophilic condition was optimum for the growth of all potato genotypes. Data were collected on plant height, number of roots and number of nodes. Maximum plant height, highest number of roots and number of nodes were observed in all genotypes grown at 25°C. In vitro microareophillic condition of the plant growth was very slow but conservation was maximum. It was concluded from the present investigation that low temperature and micro-aerophilic condition is best for in vitro conservation of International Potato Center (CIP) germplasm which can increase the period between sub culturing.

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“…y -Averages followed by the same lower case letter in each line do not differ at a 5% probability level by the Tukey test. Mohamed & Alsadon (2010) found that 87.64 mM of sucrose gave the best results in terms of the numbers and lengths of buds produced on potato explants. Romano et al (1995) observed that sucrose (87.64 mM) and glucose (222.01 mM) were the best carbon sources during the proliferation phase of the species Quercus suber.…”

Section: Resultsmentioning

confidence: 95%

“…Sucrose (at concentrations between 58.42 mM to 116.85 mM) has been the most widely used carbohydrate source for explant culturing, although other compounds such as glucose, fructose, and maltose at different concentrations may also give positive results, depending (principally) on the genotype being tested (Grattapaglia & Machado, 1998;Nicoloso et al, 2003;Mosaleeyanon et al, 2004;Skrebsky et al, 2004;Pati et al, 2006;Rodrigues et al, 2006;Bandeira et al, 2007;Luo et al, 2009;Dobránszki & Silva, 2010;Mohamed & Alsadon, 2010).…”

Section: Resultsmentioning

confidence: 99%

Effect of different carbon sources on the in vitro multiplication of Annona sp.

et al. 2011

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The Annonaceae family comprises approximately 2.300 species, some with significant commercial value. Although commercial plantations have suffered due to problems related to seedling production. As micropropagation is a viable technique for seedling production, the present work evaluated the effects of different carbon sources on in vitro bud induction in five Annonaceae species. Nodal segments obtained from plants of the Annona glabra, A. cauliflora, A. coriacea, A. bahiensis and Rollinia silvatica species were inoculated into solid WPM culture medium with 8.87 M BAP, 0.86 mM of benomyl, and 87.64 mM of the following carbon sources: glucose, sucrose, fructose, galactose, sorbitol and maltose. We evaluated the buds number, the length and weight of the largest bud, the number of expanded leaves per bud, the length of the largest leaf and the dry matter of the buds. No significant difference was observed among the different carbon sources used in terms of the number of produced buds; however, the length of the largest bud, the number of expanded leaves, the length of the largest leaf, and dry weight of the buds presented significant difference according to the studied speciesas well as the carbon sources used, with the lowest value being obtained with sorbitol. The results obtained here indicated that, except for sorbitol, any of the carbohydrates tested could be used in the in vitro multiplication protocols for A. bahiensis, A. cauliflora, A. coriacea, A. glabra and R. silvatica.Index terms: Annonaceae, micropropagation, carbohydrates. RESUMOA família Annonaceae compreende, aproximadamente, mais de 2.300 espécies, algumas com ampla aceitação no mercado nacional. No entanto, o plantio comercial tem sido prejudicado, em razão das questões relacionadas à produção de mudas. Considerando que a micropropagação é uma técnica viável nesse contexto, conduziu-se este trabalho, com o objetivo de avaliar o efeito de diferentes fontes de carbono na indução de brotações in vitro em cinco espécies de anonáceas. Segmentos nodais obtidos de plantas de Annona glabra, A. cauliflora, A. coriacea, A. bahiensis e Rollinia silvatica, foram inoculados em meio à cultura WPM sólido + 8,87 M de BAP, 0,86 mM de benomil e 87,64 mM das seguintes fontes de carbono: glucose, sucrose, fructose, galactose, sorbitol e maltose. Avaliou-se o número de brotações, o comprimento e o peso da maior brotação, o número de folhas expandidas por brotação, o comprimento da maior folha e a matéria seca das brotações. Não houve diferença significativa entre as fontes de carbono para o número de brotações. Mas, para as variáveis comprimento das brotações, número de folhas expandidas, comprimento da maior folha e matéria seca das brotações, tanto as espécies estudadas como as fontes de carbono influenciaram significativamente as respostas, e os menores valores foram obtidos na presença de sorbitol. Os resultados obtidos indicam que, exceto o sorbitol, qualquer carboidrato pode ser utilizado em protocolos de micropropagação de A. bahiensis, A. cauliflora,...

show abstract

Influence of ventilation and sucrose on growth and leaf anatomy of micropropagated potato plantlets

Cited by 74 publications

References 23 publications

Proline levels, oxidative metabolism and photosynthetic pigments during in vitro growth and acclimatization of Pitcairnia encholirioides L.B. Sm. (Bromeliaceae)

Proline levels, oxidative metabolism and photosynthetic pigments during in vitro growth and acclimatization of Pitcairnia encholirioides L.B. Sm. (Bromeliaceae)

In vitro conservation of exotic potato genotypes through different Incubated temperatures, aerophilic and micro-aerophilic conditions

Effect of different carbon sources on the in vitro multiplication of Annona sp.

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