Influence of water chemistry on the distribution of an acidophilic protozoan in an acid mine drainage system at the abandoned Green Valley coal mine, Indiana, USA

Brake, Sandra S.; Dannelly, H. Kathleen; Connors, Katherine A.; Hasiotis, Stephen T.

doi:10.1016/s0883-2927(01)00033-6

Cited by 44 publications

(31 citation statements)

References 18 publications

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“…Elongated cells with rust-colored granules were commonly observed under light microscopy, suggesting Fe sequestration (6). These cells were morphologically similar to Euglena mutabilis, which has been identified at many AMD sites (6,9). Bacterial communities found at the emergence were dominated (Ͼ50% total cells) by bacteria with morphologies characteristic of spirochetes.…”

Section: Resultssupporting

confidence: 55%

Application of a Depositional F acies Model to an Acid Mine Drainage Site

Brown

Jones

Mills

et al. 2011

Appl Environ Microbiol

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Lower Red Eyes is an acid mine drainage site in Pennsylvania where low-pH Fe(II) oxidation has created a large, terraced iron mound downstream of an anoxic, acidic, metal-rich spring. Aqueous chemistry, mineral precipitates, microbial communities, and laboratory-based Fe(II) oxidation rates for this site were analyzed in the context of a depositional facies model. Depositional facies were defined as pools, terraces, or microterracettes based on cm-scale sediment morphology, irrespective of the distance downstream from the spring. The sediments were composed entirely of Fe precipitates and cemented organic matter. The Fe precipitates were identified as schwertmannite at all locations, regardless of facies. Microbial composition was studied with fluorescence in situ hybridization (FISH) and transitioned from a microaerophilic, Euglena-dominated community at the spring, to a Betaproteobacteria (primarily Ferrovum spp.)-dominated community at the upstream end of the iron mound, to a Gammaproteobacteria (primarily Acidithiobacillus)-dominated community at the downstream end of the iron mound. Microbial community structure was more strongly correlated with pH and geochemical conditions than depositional facies. Intact pieces of terrace and pool sediments from upstream and downstream locations were used in flowthrough laboratory reactors to measure the rate and extent of low-pH Fe(II) oxidation. No change in Fe(II) concentration was observed with 60 Co-irradiated sediments or with no-sediment controls, indicating that abiotic Fe(II) oxidation was negligible. Upstream sediments attained lower effluent Fe(II) concentrations compared to downstream sediments, regardless of depositional facies.

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Section: Resultssupporting

confidence: 55%

Application of a Depositional F acies Model to an Acid Mine Drainage Site

Brown

Jones

Mills

et al. 2011

Appl Environ Microbiol

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“…First, it has been shown that the 'oversaturation' in dissolved oxygen (that is, 4200% saturation with the atmosphere) facilitates inorganic co-precipitation of arsenic and iron colloids (Brake et al, 2001a(Brake et al, , 2001b. Second, an increased concentration in dissolved oxygen may favor the development of aerobic species such as Thiomonas or Acidothiobacillus spp., both known to be dominant and involved in the bio-attenuation processes at the Carnoulès AMD Duquesne et al, 2003;Bertin et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

“…It has been described as a part of the eukaryotic microbial community in peat bogs (Pentecost, 1982), volcanic lakes (Sittenfeld et al, 2002) and acid mine drainages (AMDs) (Casiot et al, 2004a), and is considered as a bio-indicator of these latter ecosystems (Brake et al, 2001a(Brake et al, , 2001b. AMDs exposed environments are usually considered very toxic to biota as they are characterized, besides low pH values, by very high concentrations of metallic compounds (Leblanc et al, 1996).…”

Section: Introductionmentioning

confidence: 99%

In situ proteo-metabolomics reveals metabolite secretion by the acid mine drainage bio-indicator, Euglena mutabilis

et al. 2012

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Euglena mutabilis is a photosynthetic protist found in acidic aquatic environments such as peat bogs, volcanic lakes and acid mine drainages (AMDs). Through its photosynthetic metabolism, this protist is supposed to have an important role in primary production in such oligotrophic ecosystems. Nevertheless, the exact contribution of E. mutabilis in organic matter synthesis remains unclear and no evidence of metabolite secretion by this protist has been established so far. Here we combined in situ proteo-metabolomic approaches to determine the nature of the metabolites accumulated by this protist or potentially secreted into an AMD. Our results revealed that the secreted metabolites are represented by a large number of amino acids, polyamine compounds, urea and some sugars but no fatty acids, suggesting a selective organic matter contribution in this ecosystem. Such a production may have a crucial impact on the bacterial community present on the study site, as it has been suggested previously that prokaryotes transport and recycle in situ most of the metabolites secreted by E. mutabilis. Consequently, this protist may have an indirect but important role in AMD ecosystems but also in other ecological niches often described as nitrogen-limited.

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“…Protozoan communities are directly influenced by water pollution conditions (Zarda et al, 1998;Brake et al, 2001). Unlike water quality measurements, which only provide an instantaneous assessment of lake conditions, protozoa community can be used to identity past disturbances and toxic effects that are not readily detected by chemical means (Cairns and McCormick, 1993).…”

Section: Introductionmentioning

confidence: 99%

Development of the microbial communities in lake donghu in relation to water quality

Jiang

Shen

2006

Environ Monit Assess

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There is increasing recognition that protozoa is very useful in monitoring and evaluating water ecological healthy and quality. In order to study the relationship between structure and function of protozoan communities and water qualities, six sampling stations were set on Lake Donghu, a hypereutrophic subtropical Chinese lake. Microbial communities and protists sampling from the six stations was conducted by PFU (Polyurethane foam unit) method. Species number (S), diversity index (DI), percentage of phytomastigophra, community pollution value (CPV), community similarity and heterophy index (HI) were mensurated. The measured indicators of water quality included total phosphorus (TP), dissolved oxygen (DO), Chemical oxygen demand (COD), NH 4 + , NO 2 − and NO 3 − . Every month water samples from stations I, II, III, IV were chemically analyzed for a whole year, Among the chemically analyzed stations, station I was the most heavily polluted, station II was the next, stations III and IV had similar pollution degrees. The variable tendencies of COD, TP, NH 3 , NO 2 − , NO 3 − , and DO during the year was approximately coincident among the six stations. Analysis from the community parameters showed that the pollution of station 0 was much more serious than others, and station V was the most slight. Of the community parameters, CPV and HI were sensitive in reflecting the variables of the water quality. Community similarity index was also sensitive in dividing water qualities and the water quality status of different stations could be correctly classified by the cluster analysis. DI could reflect the tendency of water quality gradient, species number and percentage of Phytomastigophora was not obvious in indicating the water quality gradient.

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Influence of water chemistry on the distribution of an acidophilic protozoan in an acid mine drainage system at the abandoned Green Valley coal mine, Indiana, USA

Cited by 44 publications

References 18 publications

Application of a Depositional F acies Model to an Acid Mine Drainage Site

Application of a Depositional F acies Model to an Acid Mine Drainage Site

In situ proteo-metabolomics reveals metabolite secretion by the acid mine drainage bio-indicator, Euglena mutabilis

Development of the microbial communities in lake donghu in relation to water quality

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