Influence of weaning methods on the diaphragm after mechanical ventilation in a rat model

Bruells, Christian S.; Breuer, Thomas; Maes, Karen; Bergs, Ingmar; Berne, Christian; Marx, Gernot; Weis, Joachim; Gayan‐Ramirez, Ghislaine; Rossaint, Rolf

doi:10.1186/s12890-016-0285-2

Cited by 10 publications

(5 citation statements)

References 36 publications

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“…It has been reported that normal people stay in bed strictly for 1 day, and their muscles decrease by 1%. [31] The longer the mechanical ventilation days, the longer the patient needs to be immobilized. Studies have shown that when mechanical ventilation lasts for more than 5 days, the incidence of ICU-AW is 25% to 60%, [32] further confirming that mechanical ventilation is an independent risk factor for ICU-AW.…”

Section: Discussionmentioning

confidence: 99%

A systematic review and meta-analysis of risk factors for intensive care unit acquired weakness

Yang

Wang

et al. 2022

Medicine

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Background: The aim of this meta-analysis is to systematically evaluate and summarize the risk factors of intensive care unit acquired weakness (ICU-AW), to provide evidence-based evidence for the formulation of prevention strategies for ICU-AW.Methods: PubMed, EMBASE, Web of Science, CBM (China Biology Medicine, China), Chinese National Knowledge Infrastructure, Chinese WANFANG, and VIP will be searched to define relevant risk factors for ICU-AW. The databases search period is from January 1, 2005 to August 13, 2021. The Newcastle Ottawa Scale (NOS) is used to evaluate the quality of the included studies. RevMan 5.3 analysis software will be used for meta-analysis.Results: This systematic review and meta-analysis included a total of 12 cohort studies, including 9 international journals and 3 Chinese journals, with a total of 1950 patients, of which 856 had ICU-AW. The results showed that the significant risk factors for ICU-AW included female (odds ratio [OR] = 1.34, 95% confidence interval [CI]: 1.06-1.71; P = .02), mechanical ventilation days (

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Section: Discussionmentioning

confidence: 99%

A systematic review and meta-analysis of risk factors for intensive care unit acquired weakness

Yang

Wang

et al. 2022

Medicine

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“…After electrophoresis (7.5% polyacrylamide gel and blotting) polyclonal anti-4-HNE antibody (# MAB3249, R&D Systems, Abingdon, UK) was used as primary antibody and a polyclonal rabbit anti-mouse (# P0260, Agilent, Heverlee, Belgium) as secondary antibody. Data were expressed as a ratio between densitometric values of 4-HNE and Vinculin 15 .…”

Section: Methodsmentioning

confidence: 99%

Acute liver injury following acetaminophen administration does not activate atrophic pathways in the mouse diaphragm

Bruells

Duschner

Marx

et al. 2021

Sci Rep

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N-acetyl-para-amino phenol (APAP, usually named paracetamol), which is commonly used for its analgesic and antipyretic properties may lead to hepatotoxicity and acute liver damage in case of overdoses. Released cytokines and oxidative stress following acute liver damage may affect other organs’ function notably the diaphragm, which is particularly sensitive to oxidative stress and circulating cytokines. We addressed this issue in a mouse model of acute liver injury induced by administration of APAP. C57BL/6J mice (each n = 8) were treated with N-acetyl-para-amino phenol (APAP) to induce acute drug caused liver injury and sacrificed 12 or 24 h afterwards. An untreated group served as controls. Key markers of inflammation, proteolysis, autophagy and oxidative stress were measured in diaphragm samples. In APAP treated animals, liver damage was proven by the enhanced serum levels of alanine aminotransferase and aspartate aminotransferase. In the diaphragm, besides a significant increase in IL 6 and lipid peroxidation, no changes were observed in key markers of the proteolytic, and autophagy signaling pathways, other inflammatory markers and fiber dimensions. The first 24 h of acute liver damage did not impair diaphragm atrophic pathways although it slightly enhanced IL-6 and lipid peroxidation. Whether longer exposure might affect the diaphragm needs to be addressed in future experiments.

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“…Previous studies have demonstrated that calpain-induced myofibrillar protein degradation (Smuder et al, 2010) and calpain activity (e.g., by 95%; Bruells et al, 2016) are both increased in the diaphragm of rats undergoing mechanical ventilation. Our results showed that the protein expression of calpain-1 and calpain-2 increased by 94.6 and 47.2%, respectively, whereas that of calpastatin (an endogenous inhibitor of calpains) decreased by 32.6% in the gastrocnemius muscle of the HIB compared with the SA group (Figure 5b-d), suggesting that the upregulation of calpain signals might contribute to gastrocnemius muscle atrophy.…”

Section: Ubiquitin-proteasome Pathwaymentioning

confidence: 96%

Differential protein metabolism and regeneration in hypertrophic diaphragm and atrophic gastrocnemius muscles in hibernating Daurian ground squirrels

Xia

Gao

Niu

et al. 2021

Experimental Physiology

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Are differences in the regulation of protein metabolism and regeneration involved in the different phenotypic adaptation mechanisms of muscle hypertrophy and atrophy in hibernators? Two fast-type muscles (diaphragm and gastrocnemius) in summer active and hibernating Daurian ground squirrels were selected to detect changes in crosssectional area (CSA) and protein expression indicative of protein synthesis metabolism (protein expression of P-Akt, P-mTORC1, P-S6K1 and P-4E-BP1), protein degradation metabolism (MuRF1, atrogin-1, calpain-1, calpain-2, calpastatin, desmin, troponin T, Beclin1 and LC3-II) and muscle regeneration (MyoD, myogenin and myostatin). In the hibernation group compared with the summer active group, the CSA of the diaphragm muscle increased significantly by 26.1%, whereas the CSA of the gastrocnemius muscle decreased significantly by 20.4%. Our study also indicated that increased protein synthesis, decreased protein degradation and increased muscle regenerative potential contributed to diaphragm muscle hypertrophy, whereas decreased protein synthesis, increased protein degradation and decreased muscle regenerative potential contributed to gastrocnemius muscle atrophy. In conclusion, the differences in muscle regeneration and regulatory pattern of protein metabolism might contribute to the different adaptive changes observed in the diaphragm and gastrocnemius muscles of ground squirrels.

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Influence of weaning methods on the diaphragm after mechanical ventilation in a rat model

Cited by 10 publications

References 36 publications

A systematic review and meta-analysis of risk factors for intensive care unit acquired weakness

A systematic review and meta-analysis of risk factors for intensive care unit acquired weakness

Acute liver injury following acetaminophen administration does not activate atrophic pathways in the mouse diaphragm

Differential protein metabolism and regeneration in hypertrophic diaphragm and atrophic gastrocnemius muscles in hibernating Daurian ground squirrels

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