Influence of zeaxanthin on quantum yield of photosynthesis of Synechococcus clone WH7803 (DC2)

Bidigare, R. R.; Schofield, Oscar; Prézelin, BB

doi:10.3354/meps056177

Cited by 149 publications

(94 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…11c). Several authors have noted reduced quantum yields associated with samples containing high levels of photoprotective pigments (Bidigare et al 1989, Babin et al 1996. We know that phytoplankton from the middle of the transect contained a relatively greater proportion of the photoprotective pigment diadinoxanthin than did samples from either end of the transect (see Fig.…”

Section: Quantum Yieldmentioning

confidence: 99%

Bio-optical characteristics of diatom and prymnesiophyte populations in the Labrador Sea

Stuart¹,

Sathyendranath²,

Head³

et al. 2000

Mar. Ecol. Prog. Ser.

View full text Add to dashboard Cite

During the spring of 1996, phytoplankton samples were collected along a transect from South Wolf Island (Labrador) to Cape Desolation (Greenland). Dense blooms of diatoms were found over the shelf near the coast of Labrador, whereas high concentrations of the colony-forming prymnesiophyte Phaeocystis pouchetii were found close to Greenland. Phytoplankton samples were separated into 2 major groups (diatoms or prymnesiophytes) on the basis of chlorophyll (chl) chl c 3 /chl a ratios (determined by HPLC analysis), and the effects of species composition on the absorption and photosynthetic characteristics of these 2 high-latitude phytoplankton populations were studied. At all pigment concentrations and all wavelengths examined (apart from 623 nm), the diatom population had a much lower absorption coefficient than the prymnesiophyte population; this was attributed to an increased pigment-packaging effect in the larger diatom cells. Varying proportions of photoprotective pigments also influenced the absorption characteristics of these populations. The low specific-absorption coefficient of the diatom population resulted in a higher maximum photosynthetic quantum yield relative to that of the prymnesiophyte population. The initial slope of the photosynthesis-irradiance (P-E) curve (α B ) also appeared to be taxon-specific, with higher α B values being recorded for the smaller prymnesiophytes than for the larger diatom cells. The implications of speciesdependent variations in phytoplankton absorption coefficients for the retrieval of remotely-sensed chl a are discussed.

show abstract

Section: Quantum Yieldmentioning

confidence: 99%

Bio-optical characteristics of diatom and prymnesiophyte populations in the Labrador Sea

Stuart¹,

Sathyendranath²,

Head³

et al. 2000

Mar. Ecol. Prog. Ser.

View full text Add to dashboard Cite

show abstract

“…nique [Bidigare et al, 1989]. The in vivo weight-specific absorption coefficients of the major pigments are reconstructed on the basis of the in vitro (high-performance liquid chromatography (HPLC) generated) spectra in organic solvents, with the absorption maxima shifted to match the corresponding in vivo peaks.…”

Section: Introductionmentioning

confidence: 99%

Spatial variations in the chlorophyll‐specific absorption coefficients of phytoplankton and photosynthetically active pigments in the equatorial Pacific

Allali¹,

Bricaud²,

Claustre³

1997

J. Geophys. Res.

View full text Add to dashboard Cite

Abstract. Chlorophyll-specific absorption coefficients of particles, a • (X), and of phytoplankton, a ;h(•-), were determined using the glass-fiber filter technique along 150øW in the equatorial Pacific (13øS-løN). A site-specific algorithm for correcting the path length amplification effect was derived from field measurements. Then a decomposition technique using the high-performance liquid chromatography pigment information and taking into account the package effect was used to partition a;h into the contributions of photosynthetic pigments (a The spectrophotometer automatically corrected for the baseline which was stored prior to analyses. All spectra were set to 0 at 750 nm to minimize differences between sample and reference filters. Such an approximation, which would be questionable for detrital samples, is justified here because total absorption was largely dominated by living phytoplankton (see Figures 4a, 4b, 4e, and 4f). The optical densities measured on the filter, ODf(X), were corrected for the path length amplification effect using a site-specific algorithm, as described in the Appendix (equation (8) Partitioning Absorption into Photosynthetic and Nonphotosynthetic ComponentsThe chl a-specific absorption spectrum of phytoplankton, a,oh(X), was further partitioned into its photosynthetic and nonphotosynthetic components, a ps(X) and a np•(X) (see (1) Pigment Analyses and Other MeasurementsSamples for pigment measurements were collected on Whatman GF/F filters and either analyzed immediately or stored in liquid nitrogen for later analysis. Pigments were quantified using HPLC according to the procedure described by Vidussi et al. [1996]. The algal pigments identified and quantified include the following photosynthetic pigments: chlorophylis a, b, c, DV chl a, divinyl chlorophyll b (DV chl b), peridinin, 19'-hexanoyloxyfucoxanthin (19'-HF), 19'-butanoyloxyfucoxanthin (19'-BF), prasinoxanthin, fucoxanthin, a carotene, and three nonphotosynthetic pigments: zeaxanthin, diatoxanthin, and diadinoxanthin. The /3 carotene, which is a nonphotosynthetic pigment, was not discriminated from a carotene, which results in underestimating a* and overestimat-* Analyses previously performed on oligotrophic waters ing a p•.in the tropical North Atlantic, however, showed that the /3 carotene-to-zeaxanthin ratio was <10% throughout the water column, so that the error on these coefficients is thought to be very limited.Cell number densities for Prochlorococcus, Synechococcus, and picoeukaryots were determined using a FACSort flow cy-

show abstract

“…cyanobacteria) are known to have a difference in the composition of light-harvesting ments between the 2 photosystems, which has been shown to cause a striking difference between the shapes of the absorption and action spectra obtained using either the 14C method (Lewis et al 1986, Bidigare et al 1989, Warnock 1990 or the fluorescence excitation spectrum (Johnsen & Sakshaug 1996). Where such algae dominate, the absorption spectrum might be compromised as a proxy for the action spectrum.…”

Section: Refinements To the Model Using Measurements Of Spectral Irramentioning

confidence: 99%

“…One method circumventing the problem of NPPs is by selecting the fluorescence excitation spectrum to approximate the shape of the photosynthetic action spectrum, since NPPs do not fluoresce (Sakshaug et al 1991. Another approach is to subtract out the absorption of NPPs from the absorption spectrum of phytoplankton (Bidigare et al 1989, Bouman et al 2000 based on information on the concentration of photosynthetic and non-photosynthetic pigments and their weight-specific absorption coefficients. It is the latter approach that will be examined in this study.…”

Section: Introductionmentioning

confidence: 99%

“…In general, the shapes of the absorption spectrum and action spectrum (14C or fluorescence excitation) of phytoplankton may differ for 2 reasons: the presence of NPPs and a difference in the composition of light-harvesting pigments between the 2 photosystems (Lewis et al 1986, Bidigare et al 1989, Warnock 1990, Johnsen & Sakshaug 1996. One method circumventing the problem of NPPs is by selecting the fluorescence excitation spectrum to approximate the shape of the photosynthetic action spectrum, since NPPs do not fluoresce (Sakshaug et al 1991.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Bio-optical properties of the subtropical North Atlantic. II. Relevance to models of primary production

Bouman¹,

Platt²,

Sathyendranath³

et al. 2000

Mar. Ecol. Prog. Ser.

View full text Add to dashboard Cite

. ABSTRACT: Based on measurements of phytoplankton biomass, absorption coefficients and photosynthetic performance obtained from samples collected in the subtropical Atlantic Ocean, standard sets of bio-optical parameters were selected and applied to a spectral model of primary production. Computed profiles of instantaneous production, integrated over the day, and spectral irradiance were compared with measurements of 12 h in situ production and in situ spectral irradiance to assess the model's performance. Although the model performed well for the stations located on the western side of the transect, at the eastern side of the basin the model overestimated both primary production and irradiance below the mixed layer. The modelled irradiance profile was then reconciled with the observations of irradiance by increasing the assumed contribution to absorption by yellow substances. When the production model was re-implemented with the new irradiance values below the mixed layer, the computed and measured production profiles of all the stations were in good agreement. In the spectral model used in this study, the shape of the absorption spectrum of phytoplankton was used as a proxy for the shape of the photosynthetic action spectrum. Because non-photosynthetic pigments (NPPs) were abundant in pigment samples collected in the study region, the influence of NPPs on the shape of the absorption spectrum was examined and its effect on computations of primary production was quantified. When total aPh(z, h) and photosynthetic a,,(z, h) phytoplankton absorption at wavelength h and depth z were used to quantify the error resulting from failure to correct for the influence of NPPs on the shape of the absorption spectra, the results showed small errors in the computation of production at depth (up to 20%) and integrated, water-column primary production (a maximum of 10 %).

show abstract

Influence of zeaxanthin on quantum yield of photosynthesis of Synechococcus clone WH7803 (DC2)

Cited by 149 publications

References 23 publications

Bio-optical characteristics of diatom and prymnesiophyte populations in the Labrador Sea

Bio-optical characteristics of diatom and prymnesiophyte populations in the Labrador Sea

Spatial variations in the chlorophyll‐specific absorption coefficients of phytoplankton and photosynthetically active pigments in the equatorial Pacific

Bio-optical properties of the subtropical North Atlantic. II. Relevance to models of primary production

Contact Info

Product

Resources

About